Development of modified eggshell membrane formulations with enhanced immunomodulatory and anti-inflammatory activities to expand the non-food market for eggs.

开发具有增强免疫调节和抗炎活性的改良蛋壳膜制剂，以扩大鸡蛋的非食品市场。

• 批准号: 556438-2020
• 负责人: Hincke, Max
• 金额: $ 4.37万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2021
• 资助国家: 加拿大
• 起止时间: 2021-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=720733
• 关键词: Development; modified; eggshell; membrane; formulations

In developed countries such as Canada, about 30% of shell eggs are diverted to breaker processing plants for industrial production of egg white and yolk for the food processing and biopharmaceutical industries. However, large quantities of waste eggshell / eggshell membrane are generated for which the egg producing industry seeks high-value applications. The eggshell membrane (ESM) is approximately 1% of egg weight and consists of a bilayered proteinaceous fibrous meshwork, which is composed mainly of proteins (~90%), lipids (~3%), and carbohydrates (~2%). ESM fibers contain various structural proteins, including collagens and cysteine-rich eggshell membrane proteins (CREMPs), in addition to a significant content of bioactive proteins. ESM is considered a functionally equivalent extracellular matrix (ECM); however, it is greatly stabilized by extensive desmosine, isodesmosine, and disulfide cross-linkages, which challenge the identification of its protein constituents by standard proteomic approaches, and shield their activities. In spite of these difficulties, we have identified >500 protein constituents of ESM with numerous predicted biological functions such as antimicrobial, immunomodulatory and anti-inflammatory properties. However, specific processing approaches to yield highly active formulations of ESM material, and their mechanism of action, have not yet been satisfactorily achieved. In this Alliance grant application, we have partnered with Egg Farmers of Canada and Egg Farmers of Ontario (LRIC), as well as a major Canadian egg producer (Burnbrae Farms), to develop new processing methods to produce ESM with enhanced immunomodulatory and anti-inflammatory activities. This experimental program will lead to the development of modified ESM formulations to open new high-value markets for the Canadian poultry industry.

在加拿大等发达国家，约30%的带壳鸡蛋被转移到破碎加工厂，用于食品加工和生物制药行业的白色和蛋黄的工业化生产。然而，产生了大量的废蛋壳/蛋壳膜，蛋生产工业寻求高价值的应用。蛋壳膜（ESM）约占蛋重的1%，由双层蛋白质纤维网组成，主要由蛋白质（~90%）、脂质（~3%）和碳水化合物（~2%）组成。ESM纤维含有各种结构蛋白，包括胶原蛋白和富含半胱氨酸的蛋壳膜蛋白（CREMP），以及大量的生物活性蛋白。ESM被认为是功能等效的细胞外基质（ECM）;然而，它通过广泛的锁链素、异锁链素和二硫键交联而大大稳定，这挑战了通过标准蛋白质组学方法对其蛋白质成分的鉴定，并屏蔽了它们的活性。尽管存在这些困难，我们已经鉴定了ESM的>500种蛋白质组分，其具有许多预测的生物学功能，例如抗微生物、免疫调节和抗炎特性。然而，尚未令人满意地实现产生无害环境管理材料的高活性制剂的具体加工方法及其作用机制。在这项联盟拨款申请中，我们与加拿大蛋农和安大略蛋农（LRIC）以及加拿大主要鸡蛋生产商（Burnbrae Farms）合作，开发新的加工方法，生产具有增强免疫调节和抗炎活性的ESM。这一实验性项目将导致改良的无害环境管理配方的开发，为加拿大家禽业开辟新的高价值市场。