E-cadherin Mechanotransduction, Pluripotency and the Warburg Effect

E-钙粘蛋白机械转导、多能性和 Warburg 效应

• 批准号: RGPIN-2016-06506
• 负责人: Rancourt, Derrick
• 金额: $ 2.4万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=629476
• 关键词: cadherin; Mechanotransduction; Pluripotency; Warburg; Effect

Embryonic stem cells (ESCs) are pluripotent meaning they have the ability to differentiate into any cell type in the body. We have observed that the pluripotency of murine (m)ESCs is promoted when mESC aggregates are stirred in bioreactors. Pluripotency is maintained even when the maintenance factor LIF is removed. Moreover, cellular reprogramming, the process of dedifferentiating cells to pluripotency, occurs 1000-fold more efficiently in the bioreactor. Our preliminary data indicates that the pluripotency transcription factor β-catenin is translocated into the nucleus in response to fluid shear caused by stirring.

胚胎干细胞 (ESC) 具有多能性，这意味着它们能够分化为体内任何细胞类型。我们观察到，当在生物反应器中搅拌 mESC 聚集体时，小鼠 (m)ESC 的多能性得到促进。即使去除维持因子 LIF，多能性仍能保持。此外，细胞重编程（将细胞去分化为多能性的过程）在生物反应器中的效率提高了 1000 倍。我们的初步数据表明，多能转录因子β-连环蛋白响应搅拌引起的流体剪切而易位到细胞核中。