E-cadherin Mechanotransduction, Pluripotency and the Warburg Effect

胚胎干细胞（ESC）是多能性的，这意味着它们有能力分化成体内任何类型的细胞。我们已经观察到，当在生物反应器中搅拌mESC聚集体时，促进了鼠（m）ESC的多能性。即使在去除维持因子LIF时，也维持多能性。此外，细胞重编程，即细胞去分化为多能性的过程，在生物反应器中效率提高1000倍。我们的初步数据表明，多能性转录因子β-catenin是易位到细胞核中的流体剪切引起的搅拌响应。

Embryonic stem cells (ESCs) are pluripotent meaning they have the ability to differentiate into any cell type in the body. We have observed that the pluripotency of murine (m)ESCs is promoted when mESC aggregates are stirred in bioreactors. Pluripotency is maintained even when the maintenance factor LIF is removed. Moreover, cellular reprogramming, the process of dedifferentiating cells to pluripotency, occurs 1000-fold more efficiently in the bioreactor. Our preliminary data indicates that the pluripotency transcription factor β-catenin is translocated into the nucleus in response to fluid shear caused by stirring.