Analysis of the relationship between seed coat color genes and oil concentration in Brassica species through gene cloning

通过基因克隆分析甘蓝属植物种皮颜色基因与含油量的关系

• 批准号: 314537-2013
• 负责人: Li, Genyi
• 金额: $ 2.11万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630262
• 关键词: Analysis; relationship; between; seed; coat

Canola (Brassica napus) contributes over $14 billion to Canadian economy annually. Since yellow-seeded oilseed in Brassica species is associated with higher oil concentrations (3-5%), development of yellow-seeded canola cultivars will enhance canola oil production and significantly benefit the Canadian economy. The molecular mechanism underlying the contribution of individual seed coat color genes on oil concentration in Brassica species needs to be elucidated. After the genes underpinning natural mutations are characterized, these known genes will accelerate the development of yellow seeded B. napus canola hybrid cultivars. Ultimately, deployment of yellow-seeded canola will increase canola oil production. My objectives are to clone and characterize seed coat color genes controlling natural yellow-seeded mutations in B. rapa and determine their effect on oil concentrations under field conditions. Mapped based gene cloning will be used to identify the yellow seed coat color genes. These newly cloned seed coat color genes in B. rapa will be used to develop single gene lines in the same genetic background. These single gene lines with the same genetic background will allow characterizing effectively the relationship of seed coat color genes and oil concentrations using multiple year-location field trials, which will allow analyzing accurately the effect of each individual gene on oil concentration under natural conditions. RNA-seq technology will be used to perform gene expression profiling of developing seeds from single gene lines in the same genetic background and parental lines. This analysis will allow identifying the genes involved in the pathways related to oil accumulation during seed development. The novelty of this proposal is to perform gene functional analysis using a real crop and understand the mechanism underlying an important phenomenon in Brassica species using genomics tools. The results in gene cloning and functional analysis can be used by the canola industry to develop yellow-seeded canola cultivars and eventually to increase canola oil production in Canada.

油菜籽每年为加拿大经济贡献超过140亿美元。由于芸苔属植物的黄籽油籽含油量较高（3-5%），开发黄籽油菜籽品种将提高油菜籽产量，并显著有利于加拿大经济。油菜种皮颜色基因对含油量影响的分子机制有待进一步研究。在确定自然突变的基因基础后，这些已知基因将加速黄籽油菜杂交品种的发育。最终，黄籽菜籽油的种植将增加菜籽油的产量。我的目标是克隆和鉴定控制油菜天然黄籽突变的种皮颜色基因，并确定它们在田间条件下对油分浓度的影响。基于基因图谱的克隆技术将用于鉴定黄色种皮颜色基因。这些新克隆的油菜种皮颜色基因将用于培育具有相同遗传背景的单基因品系。这些具有相同遗传背景的单基因品系可以通过多年定位的田间试验有效地表征种皮颜色基因与含油浓度的关系，从而可以准确地分析每个基因在自然条件下对含油浓度的影响。RNA-seq技术将用于对具有相同遗传背景的单基因系和亲本系的发育种子进行基因表达谱分析。这一分析将有助于确定与种子发育过程中油脂积累相关的基因。该建议的新颖之处在于使用真实作物进行基因功能分析，并使用基因组学工具了解芸苔属物种中重要现象的机制。基因克隆和功能分析的结果可用于油菜籽产业开发黄籽油菜籽品种，最终增加加拿大的油菜籽油产量。