Transposon-mediated gene exploration in barley and other cereals

大麦和其他谷物中转座子介导的基因探索

• 批准号: RGPIN-2015-06652
• 负责人: Singh, Jaswinder
• 金额: $ 2.04万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630410
• 关键词: Transposon; mediated; gene; exploration; barley

Characterization of mutant alleles provides valuable insights into the genetics of normal physiological functions. Mutagenesis via T-DNA insertion, RNA interference and transposon tagging enable researchers to more readily study specific genes and their functions. Transposon -mediated insertional mutagenesis has advantages over other approaches for determining gene function especially where genetic transformation is not routine. Important genes involved in development were identified using several gene-tagging strategies involving Ac/Ds-mediated insertional mutagenesis. During the transposon movement, the transposon inserts into a new chromosomal location and may cause interruption in gene’s normal function. Some epialleles undergo paramutation which is an interaction between alleles and leads to a heritable expression change of one allele. Transposons have also served as building blocks for epigenetic phenomena, both at the level of single genes and across larger chromosomal regions. Studies are being conducted for the understanding these silencing mechanisms.

突变等位基因的特征为研究正常生理功能的遗传学提供了有价值的见解。通过T-DNA插入、RNA干扰和转座子标签进行突变使研究人员能够更容易地研究特定基因及其功能。转座子介导的插入突变在确定基因功能方面比其他方法更有优势，特别是在基因转化不是常规的情况下。通过几种涉及Ac/DS介导的插入突变的基因标记策略，确定了与发育有关的重要基因。在转座子运动过程中，转座子插入到一个新的染色体位置，并可能导致基因的正常功能中断。一些表观等位基因经历了等位基因之间的相互作用，从而导致一个等位基因的可遗传表达变化。转座子也是表观遗传现象的基石，无论是在单基因水平上，还是在更大的染色体区域上。目前正在进行研究，以了解这些沉默机制。