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Protein structure-based enhancement of enzyme performance for food and bioproduct applications using X-ray crystallography, protein modification and metabolic engineering methods

使用 X 射线晶体学、蛋白质修饰和代谢工程方法，基于蛋白质结构增强食品和生物产品应用中的酶性能

基本信息

批准号：
RGPIN-2016-06209
负责人：
Tanaka, Takuji
金额：
$ 2.04万
依托单位：
University of Saskatchewan
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2017
资助国家：
加拿大
起止时间：
2017-01-01 至 2018-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630606
关键词：
Protein structure based enhancement enzyme

项目摘要

The overarching goal of my DG research program is to take a structure-function approach to enhance the performance of enzymes as processing aids for food and bioproduct processing. We have worked on proline-specific peptidases in this context. Proline has a side chain attached to its nitrogen atom that makes it structurally distinct from the other amino acids and resistant to proteolysis. When foods are fermented, an accumulation of proline-containing peptides (Pro-peptides) occurs to elicit undesirable bitter tastes. Bitterness of Pro-peptides can be reduced through liberation of proline. At least 9 proline-specific peptidases are known and studied, with the smallest units of Pro-peptides being hydrolyzed by two principle enzymes: Xaa-Pro dipeptidase (X-Pase) and Pro-Xaa dipeptidase (P-Xase). X-Pases are also known to degrade organophosphorus (OP; compounds containing C-P (or S-P) bonds; used as pesticides and nerve gas agents). For this DG research (2016-2021), these two dipeptidases were chosen as model systems for investigating their structure-function relationships for applications in food and bioproduct processing. In this proposed plan, I aim to achieve the following three objectives: 1) To characterize the structure of

我的DG研究计划的总体目标是采取结构-功能方法来提高酶作为食品和生物制品加工助剂的性能。在这方面，我们研究了脯氨酸特异性肽酶。脯氨酸具有连接到其氮原子的侧链，这使得其在结构上不同于其他氨基酸并且对蛋白水解具有抗性。当食物发酵时，含脯氨酸的肽（前肽）的积累发生，引起不期望的苦味。前肽的苦味可以通过释放脯氨酸来降低。已知并研究了至少9种脯氨酸特异性肽酶，其中最小单位的前肽被两种主要酶水解：Xaa-Pro二肽酶（X-Xase）和Pro-Xaa二肽酶（P-Xase）。X-Pases也被认为可以降解有机磷（OP;含有C-P（或S-P）键的化合物;用作杀虫剂和神经毒气剂）。在这项DG研究（2016-2021）中，这两种二肽酶被选为模型系统，用于研究其在食品和生物制品加工中应用的结构-功能关系。在这一拟议计划中，我旨在实现以下三个目标：1）描述