Structure Function of Food Related Proteins
食品相关蛋白质的结构功能
基本信息
- 批准号:RGPIN-2017-04703
- 负责人:
- 金额:$ 2.33万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2017
- 资助国家:加拿大
- 起止时间:2017-01-01 至 2018-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The long-term goal of our NSERC-funded research program has been to understand the fundamental processes that explain how protein functionality is dictated by protein structure. In food systems, proteins (including enzymes) have prominent roles in texture, flavour, odour, nutrition and prophylaxis in food safety/plant pathology (e.g., antimicrobial and plant defense proteins) as well as being processing aids (e.g., chymosin coagulates milk in cheese production). The research focus of this proposal is on a group of enzymes called aspartic proteases which share highly similar structures and modes of action, that are best represented by the stomach enzyme pepsin. Despite this uniformity, a wide spectrum of different functions exist making them an ideal model to study how protein structure determines function. Building on previous research by our group and others, the proposed research program addresses fundamental knowledge gaps in two overarching areas: 1) food spoilage-related bioactive protein segments of aspartic proteases in plants; and 2) the underpinnings of aspartic protease structure tolerance to heat and alkalinity, and selectivity for particular targets. For the first research area, aspartic proteases from plants have an extra portion compared to those from non-plant sources called the plant-specific insert (PSI). The PSI is critical for plant aspartic proteases to interact with membranes, including activity against plant pathogens as part of plants’ immune responses to invading fungi. The structure and specific points of contact with membranes will be solved at the highest level of molecular detail in order to better understand the mode of action of the PSI. The second research area will entail solving the structures of a little-studied malarial aspartic protease (plasmepsin V) complexed with well-known inhibitors to narrow the possible range of binding sites, so as to direct computer-aided inhibitor design specific to plasmepsin V. Other studies will contribute to understanding how atypical aspartic proteases are able to work at extreme temperature (thermopsin from an organism found in acidic hot springs) and non-acidic pH (renin from human kidney). Thermopsin and renin will serve as templates for engineering pepsin (which is not temperature- or neutral pH-stable) by providing a means of testing the stabilizing effects of key structural features. The impact of the proposed research program will be to improve knowledge regarding AP structures and functions thereby enabling novel strategies for interventions in food-related challenges such as plant diseases and enzyme biotechnological applications.
我们 NSERC 资助的研究项目的长期目标是了解解释蛋白质功能如何由蛋白质结构决定的基本过程。在食品系统中,蛋白质(包括酶)在食品安全/植物病理学的质地、风味、气味、营养和预防方面发挥着重要作用(例如,抗菌和植物防御蛋白),并且作为加工助剂(例如,凝乳酶在奶酪生产中使牛奶凝固)。该提案的研究重点是一组称为天冬氨酸蛋白酶的酶,它们具有高度相似的结构和作用模式,最好的代表是胃酶胃蛋白酶。尽管存在这种一致性,但存在多种不同的功能,使它们成为研究蛋白质结构如何决定功能的理想模型。基于我们小组和其他人之前的研究,拟议的研究计划解决了两个总体领域的基本知识差距:1)植物中与食物腐败相关的天冬氨酸蛋白酶的生物活性蛋白片段; 2)天冬氨酸蛋白酶结构的基础,耐热和碱度以及对特定目标的选择性。对于第一个研究领域,与非植物来源的天冬氨酸蛋白酶相比,来自植物的天冬氨酸蛋白酶有一个额外的部分,称为植物特异性插入片段 (PSI)。 PSI 对于植物天冬氨酸蛋白酶与膜相互作用至关重要,包括对抗植物病原体的活性,作为植物对入侵真菌的免疫反应的一部分。结构和与膜的具体接触点将以最高水平的分子细节得到解决,以便更好地了解 PSI 的作用模式。第二个研究领域将解决一种鲜为人知的疟疾天冬氨酸蛋白酶(血浆蛋白酶 V)与众所周知的抑制剂复合的结构,以缩小可能的结合位点范围,从而指导针对血浆蛋白酶 V 的计算机辅助抑制剂设计。其他研究将有助于了解非典型天冬氨酸蛋白酶如何能够在极端温度下工作(来自酸性温泉中发现的生物体的嗜热蛋白酶)和 非酸性 pH(来自人肾的肾素)。热蛋白酶和肾素将通过提供测试关键结构特征的稳定效果的方法,作为工程胃蛋白酶(温度或中性 pH 不稳定)的模板。拟议研究计划的影响将是提高有关 AP 结构和功能的知识,从而为干预与食品相关的挑战(如植物病害和酶生物技术应用)提供新的策略。
项目成果
期刊论文数量(0)
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Yada, Rickey其他文献
Extraction, fractionation and physicochemical characterization of water-soluble polysaccharides from Artemisia sphaerocephala Krasch seed
- DOI:
10.1016/j.carbpol.2011.05.034 - 发表时间:
2011-08-15 - 期刊:
- 影响因子:11.2
- 作者:
Guo, Qingbin;Cui, Steve W.;Yada, Rickey - 通讯作者:
Yada, Rickey
In vitro starch digestibility, expected glycemic index and some physicochemical properties of starch and flour from common bean (Phaseolus vulgaris L.) varieties grown in Canada
- DOI:
10.1016/j.foodres.2008.03.013 - 发表时间:
2008-11-01 - 期刊:
- 影响因子:8.1
- 作者:
Chung, Hyun-Jung;Liu, Qiang;Yada, Rickey - 通讯作者:
Yada, Rickey
Scientific Integrity Principles and Best Practices: Recommendations from a Scientific Integrity Consortium
- DOI:
10.1007/s11948-019-00094-3 - 发表时间:
2019-04-01 - 期刊:
- 影响因子:3.7
- 作者:
Kretser, Alison;Murphy, Delia;Yada, Rickey - 通讯作者:
Yada, Rickey
Structure characterization of high molecular weight heteropolysaccharide isolated from Artemisia sphaerocephala Krasch seed
- DOI:
10.1016/j.carbpol.2011.05.018 - 发表时间:
2011-08-15 - 期刊:
- 影响因子:11.2
- 作者:
Guo, Qingbin;Cui, Steve W.;Yada, Rickey - 通讯作者:
Yada, Rickey
Yada, Rickey的其他文献
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{{ truncateString('Yada, Rickey', 18)}}的其他基金
Structure-Function Relationships of Food-Related Enzymes: Aspartic Proteases
食品相关酶的结构-功能关系:天冬氨酸蛋白酶
- 批准号:
RGPIN-2018-04598 - 财政年份:2022
- 资助金额:
$ 2.33万 - 项目类别:
Discovery Grants Program - Individual
Structure-Function Relationships of Food-Related Enzymes: Aspartic Proteases
食品相关酶的结构-功能关系:天冬氨酸蛋白酶
- 批准号:
RGPIN-2018-04598 - 财政年份:2021
- 资助金额:
$ 2.33万 - 项目类别:
Discovery Grants Program - Individual
Structure-Function Relationships of Food-Related Enzymes: Aspartic Proteases
食品相关酶的结构-功能关系:天冬氨酸蛋白酶
- 批准号:
RGPIN-2018-04598 - 财政年份:2020
- 资助金额:
$ 2.33万 - 项目类别:
Discovery Grants Program - Individual
Structure-Function Relationships of Food-Related Enzymes: Aspartic Proteases
食品相关酶的结构-功能关系:天冬氨酸蛋白酶
- 批准号:
RGPIN-2018-04598 - 财政年份:2019
- 资助金额:
$ 2.33万 - 项目类别:
Discovery Grants Program - Individual
Structure-Function Relationships of Food-Related Enzymes: Aspartic Proteases
食品相关酶的结构-功能关系:天冬氨酸蛋白酶
- 批准号:
RGPIN-2018-04598 - 财政年份:2018
- 资助金额:
$ 2.33万 - 项目类别:
Discovery Grants Program - Individual
Industry-academic partnership seed workshop for BC agri-food Sector
不列颠哥伦比亚省农业食品部门产学合作种子研讨会
- 批准号:
507654-2016 - 财政年份:2016
- 资助金额:
$ 2.33万 - 项目类别:
Connect Grants Level 2
Effect of composition and processing in vegan cheese
纯素奶酪成分和加工的影响
- 批准号:
501376-2016 - 财政年份:2016
- 资助金额:
$ 2.33万 - 项目类别:
Engage Grants Program
Structure-Function Relationships of Food-Related Proteins/Enzymes: Aspartic Proteinases
食品相关蛋白质/酶的结构-功能关系:天冬氨酸蛋白酶
- 批准号:
2281-2012 - 财政年份:2016
- 资助金额:
$ 2.33万 - 项目类别:
Discovery Grants Program - Individual
Structure-Function Relationships of Food-Related Proteins/Enzymes: Aspartic Proteinases
食品相关蛋白质/酶的结构-功能关系:天冬氨酸蛋白酶
- 批准号:
2281-2012 - 财政年份:2015
- 资助金额:
$ 2.33万 - 项目类别:
Discovery Grants Program - Individual
Structure-Function Relationships of Food-Related Proteins/Enzymes: Aspartic Proteinases
食品相关蛋白质/酶的结构-功能关系:天冬氨酸蛋白酶
- 批准号:
2281-2012 - 财政年份:2014
- 资助金额:
$ 2.33万 - 项目类别:
Discovery Grants Program - Individual
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原生动物四膜虫生殖小核(germline nucleus)体功能(somatic function)的分子基础研究
- 批准号:31872221
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