Characterization of Antigen-Adjuvant interactions by Atomic Force Microscopy

通过原子力显微镜表征抗原-佐剂相互作用

• 批准号: 507593-2017
• 负责人: Leonenko, Zoya
• 金额: $ 1.82万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=640151
• 关键词: Characterization; Antigen; Adjuvant; interactions; Atomic

Sanofi Pasteur is developing novel vaccine products, which contain antigens adsorbed to different types ofadjuvants. Characterization and comprehensive analysis of the antigen-adjuvant interactions is of greatimportance for this development. Traditional ways of characterization, however do not allow measurement ofmolecular forces acting between reagents on molecular level which is of key importance.The proposed new collaboration between Sanofi Pasteur and Dr.Zoya Leonenko's laboratory at the Universityof Waterloo has a goal to characterize adjuvant-antigen interactions by advanced biophysical methods, such asAtomic force microscopy, atomic force spectroscopy, and surface plasmon resonance, which would enhancethe knowledge of the forces involved in such interactions and would help in the understanding of the relevanceof the strength of antigen adsorption to adjuvants in Sanofi Pasteur's vaccine products. This new knowledgewill lead to new product development and will help to accelerate pharmaceutical research by providing anadvanced vaccine thus will contribute to the improvement of health of Canadian and worldwide community.

赛诺菲巴斯德正在开发新的疫苗产品，其中含有吸附到不同类型佐剂的抗原。抗原-佐剂相互作用的表征和综合分析对于这一发展至关重要。然而，传统的表征方法不允许在分子水平上测量试剂之间作用的分子力，这是非常重要的。赛诺菲巴斯德和滑铁卢大学Zoya Leonenko博士实验室之间拟议的新合作的目标是通过先进的生物物理方法表征抗体-抗原相互作用，如原子力显微镜，原子力光谱和表面等离子体共振，这将增强对这种相互作用中所涉及的力的认识，并有助于理解抗原吸附强度与赛诺菲巴斯德疫苗产品中佐剂的相关性。这一新的知识将导致新产品的开发，并将有助于加速药物研究，提供一种先进的疫苗，从而将有助于改善加拿大和世界各地的健康。