The Development & Implimentation of Instrumentation and Methodologies for the Rapid in situ Quantification of Micronutrient and Heavy Metal Content Found in Cannabis Plant Material

问题：Beleave Inc.计划生产高质量的医用大麻，用于治疗患有各种健康问题的患者，包括癌症、多发性硬化症和创伤后应激综合征。药用大麻生产商必须优化工业生产做法，以确保为植物健康和大麻素生产提供足够的微量营养素，并最大限度地减少金属杂质生物积累的任何可能性。问题的严重性：已有标准的经过验证的实验室方法用于植物矿物和重金属分析，但这些方法可能既耗时又昂贵，而且需要大量样品制备和销毁。在便携式光谱仪器的开发方面存在创新的机会。因此，迫切需要开发和验证用于工业规模大麻生产的大麻植物矿物和重金属含量的现场分析方法和相关的标准操作程序。解决方案建议：在瑞尔森大学联邦颁发的研究许可证下，我们将开发用于量化非精神活性的药用植物品种的叶、茎和花组织中的植物矿物和重金属含量的仪器和方法，以优化和评估工业化生产实践对植物矿物含量的影响。并对植物材料进行重金属含量的质量保证测试。

The Problem: Beleave Inc. plans to produce high-quality medicinal cannabis to treat patients suffering from avariety of health issues, including cancer, multiple sclerosis, and post-traumatic stress syndrome. Producers ofmedicinal cannabis must optimize industrial production practices to ensure adequate micronutrient delivery forplant health and cannabinoid production, as well as to minimize any potential for bioaccumulation of metalimpurities.Significance of the Problem: Standard validated laboratory methods exist for plant mineral and heavy metalanalysis, however these methods can be time-consuming and expensive, as well as requiring extensive samplepreparation and destruction. There exists the opportunity for innovation with respect to development ofportable spectroscopic instruments capable of in-situ field analysis of plant mineral and heavy metal content.Thus, there is an urgent need to develop and validate a method and associated standard operating procedure forthe in-situ analysis of cannabis plant mineral and heavy metal content for industrial scale cannabis production.Solutions Proposed: Working under a federally issued research license at Ryerson University, we will developinstrumentation and methodology for the quantification of plant mineral and heavy metal content from the leaf,stem and floral tissues of non-psychoactive, medicinal plant cultivars in order to optimize and evaluate impactof industrial production practices on plant mineral content, and to perform quality assurance testing of plantmaterial for heavy metal content.