Development of modified oligonucleotide probes
修饰寡核苷酸探针的开发
基本信息
- 批准号:513273-2017
- 负责人:
- 金额:$ 1.82万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Engage Grants Program
- 财政年份:2017
- 资助国家:加拿大
- 起止时间:2017-01-01 至 2018-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Oligonucleotide probes have found extensive applications in areas such as molecular biology, imaging, anddiagnostics. These probes incorporate reporters such as fluorophores, chromophores, and other modifiedreporters that allow for the visualization of target nucleic acid sequences using a wide range of techniques.While some oligonucleotide probes are prepared directly through the solid phase synthesis, many reporters areincompatible with the conditions that are used in this approach. As a result, post-synthetic modifications, i.e.conjugation reactions, are performed after solid phase synthesis to covalently link the reporters tooligonucleotides. Post-synthetic modifications are often inefficient in terms of reaction kinetics and/or yields,and involve tedious purifications steps. ACGT Corporation has an invested interest in developing noveloligonucleotides through either direct incorporation by the phosphoramidite chemistry or post-syntheticmodifications. The present proposal will develop protocols for the bioconjugation of oligonucleotides withprobes through the azido-alkyn Click chemistry.
寡核苷酸探针在分子生物学、影像学和诊断学等领域有着广泛的应用。这些探针结合了诸如荧光团、发色团和其他修饰的报告物,这些报告物允许使用广泛的技术来可视化靶核酸序列。虽然一些寡核苷酸探针直接通过固相合成来制备,但许多报告物与该方法中使用的条件不相容。因此,在固相合成后进行合成后修饰,即缀合反应,以共价连接报告子和寡核苷酸。合成后修饰在反应动力学和/或产率方面通常是低效的,并且涉及繁琐的纯化步骤。ACGT公司对通过亚磷酰胺化学直接掺入或合成后修饰开发新型寡核苷酸感兴趣。目前的建议将开发通过叠氮基-炔点击化学的寡核苷酸与探针的生物缀合的方案。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Yan, Hongbin其他文献
An X-lattice cored rectangular honeycomb with enhanced convective heat transfer performance
具有增强对流换热性能的 X 格子芯矩形蜂窝结构
- DOI:
10.1016/j.applthermaleng.2019.114687 - 发表时间:
2020-02-05 - 期刊:
- 影响因子:6.4
- 作者:
Yan, Hongbin;Zhang, Qiancheng;Lu, Tian Jian - 通讯作者:
Lu, Tian Jian
A novel anti-HER2 antibody GB235 reverses Trastuzumab resistance in HER2-expressing tumor cells in vitro and in vivo
- DOI:
10.1038/s41598-020-59818-2 - 发表时间:
2020-02-19 - 期刊:
- 影响因子:4.6
- 作者:
Shu, Mengjun;Yan, Hongbin;Zhou, Joe X. - 通讯作者:
Zhou, Joe X.
Confirmation of the Structure of Trans-Cyclic Azobenzene by X-Ray Crystallography and Spectroscopic Characterization of Cyclic Azobenzene Analogs
- DOI:
10.1002/slct.201703126 - 发表时间:
2018-03-07 - 期刊:
- 影响因子:2.1
- 作者:
Jun, Meesook;Joshi, Dhruval K.;Yan, Hongbin - 通讯作者:
Yan, Hongbin
Effects of microalgae-bacteria inoculation ratio on biogas slurry treatment and microorganism interactions in the symbiosis system
- DOI:
10.1016/j.jclepro.2022.132271 - 发表时间:
2022-05-28 - 期刊:
- 影响因子:11.1
- 作者:
Huang, Qiaoyun;Yan, Hongbin;Zhang, Qi - 通讯作者:
Zhang, Qi
Oligonucleotide Labeling Using BODIPY Phosphoramidite
- DOI:
10.1080/15257770.2010.536798 - 发表时间:
2011-01-01 - 期刊:
- 影响因子:1.3
- 作者:
Tram, Kha;Twohig, Daniel;Yan, Hongbin - 通讯作者:
Yan, Hongbin
Yan, Hongbin的其他文献
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{{ truncateString('Yan, Hongbin', 18)}}的其他基金
Chemical modification, structural and functional study of nucleic acids
核酸的化学修饰、结构和功能研究
- 批准号:
RGPIN-2020-07040 - 财政年份:2022
- 资助金额:
$ 1.82万 - 项目类别:
Discovery Grants Program - Individual
Chemical modification, structural and functional study of nucleic acids
核酸的化学修饰、结构和功能研究
- 批准号:
RGPIN-2020-07040 - 财政年份:2021
- 资助金额:
$ 1.82万 - 项目类别:
Discovery Grants Program - Individual
COVID-19: Impregnation of BODIPY photosensitizers in disposable mask fabric to reduce COVID-19 viral loading
COVID-19:在一次性口罩织物中浸渍 BODIPY 光敏剂以减少 COVID-19 病毒载量
- 批准号:
554819-2020 - 财政年份:2020
- 资助金额:
$ 1.82万 - 项目类别:
Alliance Grants
Chemical modification, structural and functional study of nucleic acids
核酸的化学修饰、结构和功能研究
- 批准号:
RGPIN-2020-07040 - 财政年份:2020
- 资助金额:
$ 1.82万 - 项目类别:
Discovery Grants Program - Individual
Encapsulated DNA barcodes as green trackers
封装 DNA 条形码作为绿色追踪器
- 批准号:
556290-2020 - 财政年份:2020
- 资助金额:
$ 1.82万 - 项目类别:
Alliance Grants
Modulation of the Properties of Nucleic Acids through Structural Modifications
通过结构修饰调节核酸的性质
- 批准号:
RGPIN-2015-05656 - 财政年份:2019
- 资助金额:
$ 1.82万 - 项目类别:
Discovery Grants Program - Individual
Modulation of the Properties of Nucleic Acids through Structural Modifications
通过结构修饰调节核酸的性质
- 批准号:
RGPIN-2015-05656 - 财政年份:2018
- 资助金额:
$ 1.82万 - 项目类别:
Discovery Grants Program - Individual
Development of potential alternative fluorophores to Sybr Green to stain double stranded DNA******
开发 Sybr Green 的潜在替代荧光团来染色双链 DNA*****
- 批准号:
534231-2018 - 财政年份:2018
- 资助金额:
$ 1.82万 - 项目类别:
Engage Grants Program
Modulation of the Properties of Nucleic Acids through Structural Modifications
通过结构修饰调节核酸的性质
- 批准号:
RGPIN-2015-05656 - 财政年份:2017
- 资助金额:
$ 1.82万 - 项目类别:
Discovery Grants Program - Individual
Modulation of the Properties of Nucleic Acids through Structural Modifications
通过结构修饰调节核酸的性质
- 批准号:
RGPIN-2015-05656 - 财政年份:2016
- 资助金额:
$ 1.82万 - 项目类别:
Discovery Grants Program - Individual
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