Development of potential alternative fluorophores to Sybr Green to stain double stranded DNA******

开发 Sybr Green 的潜在替代荧光团来染色双链 DNA*****

• 批准号: 534231-2018
• 负责人: Yan, Hongbin
• 金额: $ 1.82万
• 依托单位: Brock University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=669136
• 关键词: Development; potential; alternative; fluorophores; Sybr

Fluorescent dyes that emit light upon binding to double stranded DNA are crucial in applications such as quantitative Polymerase Chain Reactions (qPCR) and High Resolution Melt (HRM) experiments, both important techniques in molecular biology. These techniques have found a wide range of applications in life science, forensics, human health and beyond. While the dyes that are currently in use fulfill the requirements for qPCR and HRM to various degrees, many are expensive or not efficient enough for the applications. This project will investigate alternative dyes based on 1,8-naphthalimide core structures. The 1,8-naphthalimide fluorescent dyes are usually straightforward to synthesize, and some analogs have unique fluorescent properties that are suitable for qPCR and HRM applications. This research will generate 1,8-naphthalimide dyes that are readily available, and potentially suitable for both qPCR and HRM applications.

在结合双链DNA时发光的荧光染料在诸如定量聚合酶链式反应（qPCR）和高分辨率熔解（HRM）实验的应用中是至关重要的，这两种实验都是分子生物学中的重要技术。这些技术在生命科学、法医学、人类健康等领域有着广泛的应用。虽然目前使用的染料在不同程度上满足qPCR和HRM的要求，但许多染料价格昂贵或对于应用不够有效。该项目将研究基于1，8-萘酰亚胺核心结构的替代染料。1，8-萘二甲酰亚胺荧光染料通常合成简单，一些类似物具有独特的荧光特性，适用于qPCR和HRM应用。这项研究将产生容易获得的1，8-萘酰亚胺染料，并且可能适用于qPCR和HRM应用。