Developing an Ig-Seq workflow for analyzing populations of rabbit monoclonal antibodies

开发用于分析兔单克隆抗体群体的 Ig-Seq 工作流程

• 批准号: 522102-2017
• 负责人: Willerth, Stephanie
• 金额: $ 1.82万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=640367
• 关键词: Developing; Ig; Seq; workflow; analyzing

This project will initiate a first time collaboration between the Willerth laboratory at the University of Victoriaand Immunoprecise Antibodies Ltd. located in Victoria, B.C. Immunoprecise Antibodies Ltd. is a localbiotechnology company with over 25 years of experience in the custom antibody business. One of their majorproducts includes their proprietary technology for generating custom rabbit monoclonal antibodies using theirtechnology, which screens the immune repertoire of the rabbit for selecting the desired antibody directly fromB cells. The major goal of this Engage project is to develop a work flow for analyzing the antibody populationsproduced in these rabbit systems using next generation sequencing as a way to streamline the antibodyproduction process. The major challenges will be 1) obtaining sequencing data from the population of immunecells and 2) analyzing the resulting sequence data using bioinformatics. The Willerth lab will provide personneland expertise to achieve these goals. This project will train highly qualified personnel in the areas ofbiotechnology, cell culture, and bioinformatics.

该项目将启动维多利亚大学 Willerth 实验室与位于不列颠哥伦比亚省维多利亚的Immunprecise Antibodies Ltd. 之间的首次合作。 Immunoprecise Antibodies Ltd.是一家本地生物科技公司，在定制抗体业务方面拥有超过25年的经验。他们的主要产品之一包括使用他们的技术生成定制兔单克隆抗体的专有技术，该技术筛选兔子的免疫库，以直接从 B 细胞中选择所需的抗体。该 Engage 项目的主要目标是开发一个工作流程，使用下一代测序来分析这些兔子系统中产生的抗体群体，作为简化抗体生产过程的一种方式。主要挑战是 1) 从免疫细胞群中获取测序数据，2) 使用生物信息学分析所得序列数据。威勒斯实验室将提供人员和专业知识来实现​​这些目标。该项目将培养生物技术、细胞培养和生物信息学领域的高素质人才。