Validation of functionalized transcription factors as a novel cell culture supplements

功能化转录因子作为新型细胞培养补充剂的验证

• 批准号: 508698-2017
• 负责人: Willerth, Stephanie
• 金额: $ 1.39万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=677038
• 关键词: Validation; functionalized; transcription; factors; novel

The market for cell culture media and supplements is estimated to be $6 billion per year as reported by Global Industry Analysts Inc. The overall goal of this project is to validate novel protein therapeutics consisting of transcription factors functionalized with a novel intracellular protein delivery technology developed by iProgen Biotech for use as cell culture supplements. This project represents a continuation of a successful collaboration between Dr. Stephanie Willerth, a Canada Research Chair in Biomedical Engineering and Associate Professor of Biomedical Engineering at the University of Victoria, and the biotechnology company - iProgen Biotech. Our previous collaboration validated a novel protein therapeutic called IASCL1 for use as a cell culture supplement that promoted the differentiation of human induced pluripotent stem cells into neurons, which are cells found in the central nervous system. We also have promising preliminary data that IASCL can directly reprogram skin cells called fibroblasts into neuron-like cells. This project will further characterize ability of IASCL1 to reprogram fibroblasts into neurons as well as characterize the effects of other novel transcription factors, including ISOX2, IBRN2, IMYT1L, and INGN2, on the reprogramming process.

根据Global Industry Analyses Inc.的报告，细胞培养基和补充剂的市场估计为每年60亿美元。该项目的总体目标是验证由转录因子组成的新型蛋白质疗法，该转录因子通过iProgen Biotech开发的新型细胞内蛋白质递送技术来用作细胞培养补充剂。该项目是加拿大生物医学工程研究教授、维多利亚大学生物医学工程副教授斯蒂芬妮·威勒斯博士与生物技术公司iProgen Biotech之间成功合作的延续。我们之前的合作验证了一种名为IASCL1的新型蛋白质疗法，用于细胞培养补充，促进人类诱导的多能干细胞分化为神经元，神经元是在中枢神经系统中发现的细胞。我们也有很有希望的初步数据表明，IASCL可以直接将称为成纤维细胞的皮肤细胞重新编程为神经元样细胞。该项目将进一步表征IASCL1将成纤维细胞重新编程为神经元的能力，以及其他新的转录因子，包括ISOX2、IBRN2、IMYT1L和INGN2对重新编程过程的影响。