Impact of fungicide treatment on microbial communities associated with late season root rot in canola

拜耳作物科学公司为加拿大农民提供作物保护解决方案，主要商业重点是为加拿大油菜籽生产商提供管理选择，以保护油菜籽在种子萌发和整个生长季节免受病原体的侵害。草原油菜籽生产者正在遇到一个新出现的问题，即晚季根腐病，这可能给生产者带来重大的产量和经济损失（CanolaWatch, 2014）。晚季病理出现在传统种子处理的有效期之后，使得这些处理在控制晚季根腐病方面无效。之前的一项Engage资助利用Yost实验室的专业知识和基础设施，量化了与患有晚季根腐病的油菜植物相关的微生物群落。结果表明，eptosphaeriacaea家族真菌与植物组织病理关系最为密切，与orchobacterum和Shingobacterium菌群关系密切。从Engage项目中生成的数据集为通过Engage Plus项目继续进行这项研究提供了必要的工具。目前的提案将研究由拜耳作物科学公司开发的一种实验性杀菌剂对处理过的油菜籽作物微生物群落结构的影响，这些油菜籽作物生长在以前受晚季根腐病影响的田间地块上。结果将提供微生物群落的高分辨率量化，并了解微生物群落成员如何受到实验化合物的影响。这些数据将使拜耳作物科学公司更好地了解实验杀菌剂在预防晚季根腐病中的作用机制及其对本地土壤和内生微生物群的影响。该结果将直接有利于拜耳作物科学公司推进其害虫管理产品的开发工作。

Bayer CropScience provides crop protection solutions to Canadian farmers, with a large commercial focus forproviding Canadian canola producers with management options to protect canola crops from pathogens, duringseed emergence and throughout the growing season. Prairie canola producers are encountering an emergingproblem of late season root rot, which can result in significant yield and economic losses to producers (CanolaWatch, 2014). The late season pathology appears after the efficacy period of conventional seed treatments,rendering these treatments ineffective in the control of late season root rot. A previous Engage grant used theexpertise and infrastructure of the Yost lab to quantify the microbial communities that are associated withcanola plants suffering from late season root rot symptoms. It was found that fungal organisms belonging to theLeptosphaeriacaea family were most closely associated with plant tissue pathology, and were notablyassociated with bacterial groups of Orchobactrum and Shingobacterium. The data-sets generated from theEngage project provides the necessary tools for continuing this research through the Engage Plus program. Thecurrent proposal will study the effects of an experimental fungicide, in development by Bayer CropScience, onthe microbial community structure of treated canola crops that are grown in field plots previously impacted bylate season root rot. The results will provide a high-resolution quantification of the microbial communities andan understanding of how the microbial community members are impacted by the experimental compound.This data will provide Bayer CropScience with a greater understanding of the mechanisms of action of theexperimental fungicide in preventing late season root rot and its impact on the indigenous soil and endophyticmicrobiota. The results will be of direct benefit to Bayer CropScience by advancing their pest managementproduct development efforts.