Investigating the molecular mechanisms of plant growth promotion in canola, by Delftia acidovorans

Lallemand制造商BioBoost，一种土壤活性生长促进根际细菌（Delftia acidovorans），作为紧急后喷雾应用于油菜。在2007年至2012年的农业田间试验中，BioBoost的应用导致每英亩平均增产2蒲式耳。该项目是Lallemand和萨斯喀彻温省里贾纳大学的研究人员合作的结果，旨在研究BioBoost接种剂促进植物生长的遗传基础。该项目涉及使用高通量DNA测序对Delftia acidovorans菌株的基因组进行测序。组装的基因组将成为后续研究D.油菜根际的食酸菌定殖和植物生长促进作用。使用高通量诱变方法（INSeq）进行全基因组筛选，我们将确定在根际环境中定植的健身所需的基因。同时，RNA-seq分析将用于研究D.食酸菌，因为它殖民根际。这两种方法将为深入研究根际定殖机制和根际代谢活性提供高分辨率的信息。可能与植物生长促进相关的食酸菌。该项目的第二阶段将涉及使用INSeq和定向诱变来研究D.使用嗜酸菌接种菌株，并测量作为植物生长促进因子的硫氧化的程度。

Lallemand manufacturers BioBoost, a soil-active growth-promoting rhizobacteria (Delftia acidovorans) that is applied to canola as a post-emergent spray. In agricultural field trials between 2007 and 2012 BioBoost application resulted in an average yield increase of two bushels per acre. This project is the result of a collaboration between Lallemand and researchers at the University of Regina, Saskatchewan to investigate the genetic basis of the plant growth promotive effects of the BioBoost inoculant. The project involves the use of high throughput DNA sequencing to sequence the genome of the Delftia acidovorans strain. The assembled genome will form the basis of subsequent investigations on the mechanisms of D. acidovorans colonization of the canola rhizosphere and plant growth promotive effects. Using a high throughput mutagenesis approach (INSeq) to undertake a genome wide screen we will identify genes required for fitness in colonizing the rhizosphere environment. Simultaneously RNA-seq profiling will be used to study the transcriptome of D. acidovorans as it colonizes the rhizosphere. Together, these two methodologies will provide high resolution insights into the mechanisms of rhizosphere colonization and the metabolic activities of D. acidovorans that may be correlated to plant growth promotion. The second phase of the project will involve using INSeq and directed mutagenesis to study sulphur oxidation gene networks in the D. acidovorans inoculant strain and measure the magnitude of sulphur oxidation as a plant growth promotive factor.**