Using functional genomics to connect microbial community functional responses in an advanced wastewater treatment plant during seasonal temperature changes.

使用功能基因组学连接先进废水处理厂在季节性温度变化期间微生物群落的功能反应。

• 批准号: 529391-2018
• 负责人: Yost, Christopher
• 金额: $ 1.82万
• 依托单位: University of Regina
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=669376
• 关键词: Using; functional; genomics; connect; microbial

Wastewater treatment plants (WWTPs) are essential in limiting negative anthropogenic effects on the**environment by minimizing the release of nitrogen, phosphorus and bacteria. Large-scale WWTPs use a**combination of physical, chemical, and biological means to remove nutrients from wastewater. The biological**component of this process is completely dependent on the metabolism of microorganisms, which is**dramatically impacted by temperature. WWTPs are often built with certain components of the treatment**process outdoors, which leaves them exposed to seasonal fluctuations in temperature. In northern regions such**as the Canadian prairies where air temperatures can range from +30°C in the summer to frequent <-25°C in the**winter, treatment is dramatically impacted and nutrient removal efficiency plummets. WWTPs adapt to**fluctuating conditions by manipulating the oxygen input to favor certain microbes and by increasing the**duration of biological treatment, known as "sludge age". Though these adjustments are aimed at compensating**for the colder temperatures, they can lead to the proliferation of problematic protozoa and bacteria that disrupt**the treatment process. Currently, problems with biological nutrient removal (BNR) are diagnosed through**direct microscopy and occasionally through culture-dependent approaches. Though these methods are**undoubtedly useful, they can only provide a limited snapshot of the complexities of WWTP communities. To**better understand the microbial communities in Epcor's Regina WWTP, and the conditions that lead to**decreased operational efficiency of the biological nutrient removal process, two specific questions will be**addressed during this research project: i. How do the microbial communities change throughout the biological**treatment process and ii. How do these communities differ in warm and cold temperatures?

污水处理厂(WWTP)通过最大限度地减少氮、磷和细菌的释放，在限制对**环境的负面人为影响方面至关重要。大型污水处理厂使用物理、化学和生物方法的组合来去除废水中的营养物质。这一过程的生物学成分完全依赖于微生物的新陈代谢，而微生物的新陈代谢受温度的影响很大。污水处理厂的建造通常带有室外处理**过程的某些组件，这使它们暴露在季节性温度波动中。在北方地区，如加拿大大草原，气温从夏季的+30°C到冬季的&25°C不等，处理受到极大影响，养分去除效率直线下降。污水处理厂通过控制氧气输入以有利于某些微生物，并通过增加生物处理的持续时间来适应**波动的条件，这就是我们所说的“污泥年龄”。虽然这些调整旨在补偿**较低的温度，但它们可能会导致有问题的原生动物和细菌的繁殖，从而扰乱**治疗过程。目前，生物养分去除(BNR)的问题是通过**直接显微镜诊断的，偶尔也通过依赖培养的方法来诊断。尽管这些方法无疑是有用的，但它们只能提供垃圾处理厂社区复杂性的有限快照。为了**更好地了解Epcor的Regina污水处理厂中的微生物群落，以及导致生物养分去除过程的运行效率**降低的条件，本研究项目将**解决两个具体问题：i.微生物群落在生物**处理过程和ii.这些社区在温暖和寒冷的温度下有什么不同？