A circular dichroism spectropolarimeter for protein biophysics, self-assembly, and engineering.

用于蛋白质生物物理学、自组装和工程的圆二色性分光计。

• 批准号: RTI-2018-00246
• 负责人: Rainey, Jan
• 金额: $ 10.82万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642151
• 关键词: circular; dichroism; spectropolarimeter; protein; biophysics

This grant will be used to provide a research-grade circular dichroism (CD) spectrophotometer allowing new avenues of protein science and engineering research at Dalhousie University. Proteins may either be in a spaghetti-like (“unfolded”) chain or they may have a defined, "folded" structure. CD spectroscopy allows a relatively fast and robust characterization of the amount of unfolded vs. folded protein present under a given set of conditions. Furthermore, the actual nature of the folded state is directly reflected in the CD spectrum. All four of the co-applicants and their current roster of 16 trainees, ranging from undergraduate students developing research expertise to senior postdoctoral fellows, will be able to immediately take advantage of this instrument for fundamental studies of proteins critical for life in diverse organisms including humans, fungi, and spiders.

这笔赠款将用于提供研究级圆二色(CD)分光光度计，从而为达尔豪西大学的蛋白质科学和工程研究开辟新的途径。蛋白质可能处于意大利面条状(“未折叠”)链中，也可能具有明确的“折叠”结构。CD光谱学可以相对快速和稳健地表征在给定条件下存在的未折叠蛋白质与折叠蛋白质的数量。此外，折叠状态的实际性质直接反映在CD光谱中。所有四名共同申请者和他们目前的16名学员名单，从培养研究专长的本科生到高级博士后研究员，都将能够立即利用这一仪器对包括人类、真菌和蜘蛛在内的不同生物体中对生命至关重要的蛋白质进行基础研究。