High-throughput single-cell imaging flow cytometry platform

该提案是获得ImageStreamX Mark II，这是一种混合仪器，能够在流式细胞术分选时实时收集单个细胞的显微数据。这项强大的新技术将建立在达尔豪斯医学院核心设施基础设施的基础上，并有可能加强加拿大大西洋研究界实验室的研究项目。******该仪器将支持自然科学和工程实验室的战略研究目标，允许高分辨率监测对环境线索有不同反应的细胞群体，以及稀有细胞群体的功能表征。该仪器还将支持跨领域的研究目标，包括测量果蝇、微生物真核生物和初级神经元的基本细胞特性，以及分析构成微生物组样本的细菌和病毒。这将是加拿大大西洋地区的第一个此类工具，并将为该区域的许多受训人员提供以前无法获得的培训机会。****** 7名申请者和他们的41名学员，从本科生到博士后和专业研究助理，来自达尔豪斯大学自然科学、农业、工程和卫生部门的各个领域。然而，我们在基础生物学问题上有着共同的研究兴趣，这些问题需要对大量单细胞事件进行同时成像和流式细胞术分析。该仪器将使我们能够描述在动物、植物、细菌和病毒中发生的基本生物现象中涉及的关键参与者的特征。******所要求的设备将被安置在Dalhousie的流式细胞仪核心设施中，该设施由训练有素的专家维护和操作，他们将帮助进行实验设计，分析和故障排除。学员将获得实践经验和宝贵的专业知识与这一重要的细胞成像技术。所有申请者以及达尔豪斯和大西洋地区的许多其他研究人员都可以随时使用这些设备和相关的专业知识，这将直接支持和加强正在进行的和新的研究和培训计划。使用所要求的成像流式细胞仪系统进行的研究将为细胞反应的个性、进化生物学、神经学以及宿主及其相关微生物的反应提供重要和新颖的见解。**

This proposal is to acquire an ImageStreamX Mark II, a hybrid instrument capable of collecting microscopic data on individual cells in real time as they are sorted by flow cytometry. This powerful new technology will build on Dalhousie's Faculty of Medicine Core Facilities infrastructure and has the potential to supercharge the research programs of laboratories within the Atlantic Canada research community. ******The instrument will support strategic research goals of natural science and engineering laboratories by allowing high-resolution monitoring of populations of cells with diverse responses to environmental cues, as well as functional characterization of rare cell populations. The instrument will also support cross-cutting research objectives, including measurement of basic cellular properties in fruit flies, microbial eukaryotes and primary neurons, and analysis of the bacteria and viruses comprising microbiome samples. This instrument will be first of its kind in Atlantic Canada and will provide previously unavailable training opportunities to numerous trainees in the region.******The 7 applicants and their 41 trainees, ranging from undergraduates to postdoctoral fellows and professional research associates, are from a diverse range of natural science, agriculture, engineering, and health departments at Dalhousie University. However, we share common research interests in basic biological questions that require simultaneous imaging and flow cytometric analysis of large numbers of single cell events. The instrument will allow us to characterize key players involved in fundamental biological phenomenon occurring in animals, plants, bacteria, and viruses.******The requested equipment will be housed in Dalhousie's Flow Cytometry Core Facility, which is maintained and operated by trained experts who will aid in experimental design, analysis and trouble shooting. Trainees will gain both hands-on experience and valuable expertise with this important cellular imaging technique. The ready access of this equipment and associated expertise to all applicants and to many other researchers at Dalhousie and in the Atlantic region will directly support and enhance ongoing and new research and training programs. The studies performed with the requested imaging flow cytometry system will provide important and novel insights into the individuality of cellular responses, evolutionary biology, neurology, and the responses of both hosts and their associated microbes. **