High-throughput single-cell imaging flow cytometry platform

该提案是为了获得ImageStreamX Mark II，这是一种混合仪器，能够在通过流式细胞术分选单个细胞时真实的收集单个细胞的显微镜数据。这项强大的新技术将建立在达尔豪西医学院核心设施基础设施的基础上，并有可能为加拿大大西洋研究界的实验室研究项目提供支持。 ** 该仪器将支持自然科学和工程实验室的战略研究目标，允许高分辨率监测对环境线索有不同反应的细胞群，以及稀有细胞群的功能表征。该仪器还将支持跨领域的研究目标，包括测量果蝇、微生物真核生物和初级神经元的基本细胞特性，以及分析构成微生物组样本的细菌和病毒。该仪器将是加拿大大西洋地区的首个此类仪器，并将为该地区的众多学员提供以前无法获得的培训机会。* 7名申请人和他们的41名实习生，从本科生到博士后研究员和专业研究助理，来自达尔豪西大学的自然科学、农业、工程和卫生部门。然而，我们在基础生物学问题上有着共同的研究兴趣，这些问题需要同时对大量单细胞事件进行成像和流式细胞术分析。该仪器将使我们能够表征参与动物，植物，细菌和病毒中发生的基本生物现象的关键参与者。要求的设备将存放在达尔豪西的流式细胞术核心设施中，该设施由经过培训的专家维护和操作，这些专家将帮助进行实验设计、分析和故障排除。学员将获得这一重要的细胞成像技术的实践经验和宝贵的专业知识。所有申请人和达尔豪西和大西洋地区的许多其他研究人员都可以随时获得这种设备和相关的专门知识，这将直接支持和加强正在进行的和新的研究和培训方案。所要求的成像流式细胞术系统进行的研究将提供重要的和新颖的见解的个性细胞反应，进化生物学，神经学，以及主机和它们的相关微生物的反应。**

This proposal is to acquire an ImageStreamX Mark II, a hybrid instrument capable of collecting microscopic data on individual cells in real time as they are sorted by flow cytometry. This powerful new technology will build on Dalhousie's Faculty of Medicine Core Facilities infrastructure and has the potential to supercharge the research programs of laboratories within the Atlantic Canada research community. ******The instrument will support strategic research goals of natural science and engineering laboratories by allowing high-resolution monitoring of populations of cells with diverse responses to environmental cues, as well as functional characterization of rare cell populations. The instrument will also support cross-cutting research objectives, including measurement of basic cellular properties in fruit flies, microbial eukaryotes and primary neurons, and analysis of the bacteria and viruses comprising microbiome samples. This instrument will be first of its kind in Atlantic Canada and will provide previously unavailable training opportunities to numerous trainees in the region.******The 7 applicants and their 41 trainees, ranging from undergraduates to postdoctoral fellows and professional research associates, are from a diverse range of natural science, agriculture, engineering, and health departments at Dalhousie University. However, we share common research interests in basic biological questions that require simultaneous imaging and flow cytometric analysis of large numbers of single cell events. The instrument will allow us to characterize key players involved in fundamental biological phenomenon occurring in animals, plants, bacteria, and viruses.******The requested equipment will be housed in Dalhousie's Flow Cytometry Core Facility, which is maintained and operated by trained experts who will aid in experimental design, analysis and trouble shooting. Trainees will gain both hands-on experience and valuable expertise with this important cellular imaging technique. The ready access of this equipment and associated expertise to all applicants and to many other researchers at Dalhousie and in the Atlantic region will directly support and enhance ongoing and new research and training programs. The studies performed with the requested imaging flow cytometry system will provide important and novel insights into the individuality of cellular responses, evolutionary biology, neurology, and the responses of both hosts and their associated microbes. **