Effects of iron chelator on dynamics of bacterial biofilm

铁螯合剂对细菌生物膜动力学的影响

• 批准号: 536209-2018
• 负责人: Cheng, Zhenyu
• 金额: $ 1.82万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=647467
• 关键词: Effects; iron; chelator; dynamics; bacterial

Iron has different functions in living beings. It is required as an irreplaceable trace element by all**cells. Iron scavenging agents (chelators) are able to modulate the response of the immune system to triggers like inflammation or infection. However, a better understanding of the discrete roles that iron and its sequestration by chelators plays at the basic cellular level is needed. Chelation Partners Inc. (CPI) has developed a family of novel iron chelators. The availability of those highly-specific iron scavenging substances makes it possible for the first time to investigate the different roles that iron plays in bacterial physiology. ****The possible application of DIBI in inhibiting another important form of bacterial growth within biofilms, thereby alleviating the problems caused by biofilm growth of bacteria, has not been previously explored. The company (CPI) sees tremendous potential for new markets in developing DIBI into a product that will inhibit bacterial biofilm in the environments, but they lack the in-house capability to carry out the biofilm assays to understand the underlying mechanisms involved in DIBI. CPI requires the assistance and expertise in Microbiology and Cell Biology in Dr. Cheng's laboratory in the Department of Microbiology and Immunology at Dalhousie University.****The proposed NSERC Engage project is directed toward thoroughly examining how DIBI affects biofilm formation or enables biofilm disruption. More specifically, we will use multiple well-developed P. aeruginosa biofilm assays to measure the biomass of biofilm and viability of bacterial cells within biofilms.****In summary, this work will contribute to our understanding of the molecular basis of iron chelation in bacterial biofilm and contribute in the long run to improve aquaculture, environmental cleanup, and medical practices. ********** ****

铁在生物体中具有不同的功能。它是所有 ** 细胞所必需的不可替代的微量元素。铁清除剂（螯合剂）能够调节免疫系统对炎症或感染等触发因素的反应。然而，需要更好地理解铁及其螯合剂在基本细胞水平上起的作用。螯合合作伙伴公司(CPI)开发了一系列新颖的铁螯合剂。这些高度特异性的铁清除物质的可用性使得首次研究铁在细菌生理学中发挥的不同作用成为可能。 * DIBI在抑制生物膜内另一种重要形式的细菌生长，从而减轻由细菌的生物膜生长引起的问题中的可能应用先前尚未被探索。该公司（CPI）看到了将DIBI开发成一种抑制环境中细菌生物膜的产品的巨大市场潜力，但他们缺乏进行生物膜测定以了解DIBI所涉及的潜在机制的内部能力。CPI需要达尔豪西大学微生物学和免疫学系郑博士实验室的微生物学和细胞生物学方面的协助和专业知识。*拟议的NSERC Engage项目旨在彻底研究DIBI如何影响生物膜形成或使生物膜破裂。更具体地说，我们将使用多种成熟的铜绿假单胞菌生物膜测定来测量生物膜的生物量和生物膜内细菌细胞的活力。总之，这项工作将有助于我们了解细菌生物膜中铁螯合的分子基础，并从长远来看有助于改善水产养殖，环境净化和医疗实践。 ********** ****