Photoresponsive siRNAs as a biotechnological tool to examine gene function

光响应 siRNA 作为检查基因功能的生物技术工具

• 批准号: 531901-2018
• 负责人: Desaulniers, JeanPaul
• 金额: $ 9.07万
• 依托单位: University of Ontario Institute of Technology
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=649060
• 关键词: Photoresponsive; siRNAs; biotechnological; tool; examine

siRNAs are gene-silencing oligonucleotides that have an important role in biotechnology and as potential next**generation therapeutics. Within life sciences, siRNAs are important to elucidate gene function and characterize**complex biological pathways. One of the challenges in using siRNAs involves controlling its use once**administered inside of the cell. Azobenzene is a photoswitchable molecule that can isomerize between two**forms, cis and trans in the presence of UV and visible light, respectively. We have developed siRNAzos, that**are gene-silencing siRNAs that contain azobenzene. We have shown that siRNAzos can be reversibly activated**and inactiavted within cells with visible and UV light, respectively. We have also developed a tetrachlorinated**azobenzene that is capable of isomerizing between cis and trans with different colored light. In this phase of**the proposal, we will develop a dual siRNAzo system that targets different genes, and the two siRNAzos once**transfected in cells, can be selectively activated and inactivated with different wavelengths. This will give**researchers access to new biotechnological tools by providing them a platform for controlled gene-silencing.**This system will facilitate the characterization of complex biological pathways.

siRNA是基因沉默寡核苷酸，在生物技术中具有重要作用，并作为潜在的下一代治疗剂。在生命科学中，siRNA对于阐明基因功能和表征复杂的生物学途径非常重要。使用siRNA的挑战之一涉及一旦在细胞内施用就控制其使用。偶氮苯是一种可光转换的分子，在紫外光和可见光的存在下，可以分别在顺式和反式两种 ** 形式之间异构化。我们已经开发了siRNAzos，** 是含有偶氮苯的基因沉默siRNA。我们已经证明，siRNAzos可以在细胞内分别用可见光和紫外光可逆地激活 ** 和失活。我们还开发了一种四氯化 ** 偶氮苯，能够在不同色光下进行顺式和反式异构化。在这一阶段的提案中，我们将开发一种靶向不同基因的双siRNAzo系统，这两种siRNAzo一旦转染到细胞中，就可以用不同的波长选择性地激活和灭活。这将为 ** 研究人员提供一个可控基因沉默的平台，从而使他们能够获得新的生物技术工具。该系统将有助于表征复杂的生物途径。