Synthesis and Applications of Chemically-Modified Oligonucleotides

化学修饰寡核苷酸的合成及应用

• 批准号: RGPIN-2019-04692
• 负责人: Desaulniers, JeanPaul
• 金额: $ 2.11万
• 依托单位: University of Ontario Institute of Technology
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=765026
• 关键词: Synthesis; Applications; Chemically; Modified; Oligonucleotides

Nucleic acids are the main building blocks of DNA and RNA, which are biomacromolecules with vast biological function.  For example, nucleic acids contain the genetic blue print of the cell, and transfer of this genetic code to messenger RNA is necessary to direct the synthesis of unique proteins.  Nucleic acids also have other biological functions including controlling the expression of particular genes.  Since the discovery of the double helical structure of DNA 65 years ago, there have been significant advances in the nucleic acid field to improve its properties for biotechnological applications or for modern medicine.  For instance,  gene-silencing oligonucleotides are tools used to understand gene function. However, limitations continue due to the native structure of these molecules. Using organic chemistry, my research program develops synthetic analogs in order to improve its biological function.  My research group then evaluates the biological impact of these synthetic analogs.  The primary focus of my research group involves synthesizing novel chemically-modified oligonucleotides to use as gene-silencing oligonucleotides. First, we will explore the effect of expanding small molecules within a class of gene silencing molecules called short interfering RNAs (siRNAs). My lab has identified an area within siRNAs that is amenable to diverse chemical modification. As such, we will expand the scope and place molecules that can interact directly with different cellular biological components. When bound, these molecules may exhibit improved potency, cell-membrane permeability, and stability. Secondly, we will expand the scope of photoresponsive groups within siRNAs.  Our lab has shown that the photoresponsive molecule, azobenzene, when placed within the core central region of siRNAs can be used to reversibly control the timing of gene silencing activity.  Azobenzene can isomerize between a trans and cis form in the presence of visible and ultra-violet light, respectively.  We will expand the scope of photoresponsive RNA groups, and explore functionalized azobenzene derivatives within siRNAs. This will allow us to fine-tune the activity of siRNAs, and allow us to better control its activity, and thus prevent off-target effects. Finally, my research group has expertise in developing novel backbone modifications for oligonucleotides. We will expand the scope of these modifications and explore their effect within the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 editing system. The CRISPR/Cas9 system is an exciting, and relatively new system that has been shown to be amenable within a variety of biological systems. The multidisciplinary laboratory work is conducted by a talented team of undergraduate and graduate students who use a combination of solution-phase, solid-phase and biological techniques. As such, the trainees supervised from my lab are well prepared for a variety of professions including careers focused in chemistry and science.

核酸是DNA和RNA的主要组成部分，DNA和RNA是具有巨大生物学功能的生物大分子，例如，核酸包含细胞的遗传蓝图，而将这种遗传密码转移到信使RNA是指导独特蛋白质合成所必需的。核酸还具有其他生物学功能，包括控制特定基因的表达。自从发现双链RNA以来，DNA的螺旋结构65年前，核酸领域取得了重大进展，以改善其在生物技术应用或现代医学中的特性。例如，基因沉默寡核苷酸是用于了解基因功能的工具。然而，由于这些分子的天然结构，限制继续存在。利用有机化学，我的研究项目开发合成类似物，以改善其生物功能。我的研究小组然后评估这些合成类似物的生物影响。我的研究小组的主要重点是合成新型化学修饰的寡核苷酸，用作基因沉默寡核苷酸。首先，我们将探索在一类称为短干扰RNA（siRNA）的基因沉默分子中扩增小分子的效果。我的实验室已经确定了siRNAs中的一个区域，可以进行各种化学修饰。因此，我们将扩大范围，并放置可以直接与不同细胞生物成分相互作用的分子。当结合时，这些分子可以表现出改善的效力、细胞膜渗透性和稳定性。其次，我们将扩大siRNA中光响应基团的范围。我们的实验室已经证明，光响应分子偶氮苯，当被置于siRNA的核心中心区域时，可以用来可逆地控制基因沉默活性的时间。偶氮苯在可见光和紫外光的存在下可以在反式和顺式之间异构化，我们将扩大光响应RNA基团的范围，并探索siRNA中的功能化偶氮苯衍生物。这将使我们能够微调siRNA的活性，并使我们能够更好地控制其活性，从而防止脱靶效应。最后，我的研究小组在开发寡核苷酸的新型骨架修饰方面具有专业知识。我们将扩大这些修饰的范围，并探索它们在CRISPR（成簇的规则间隔短回文重复序列）/Cas9编辑系统中的作用。CRISPR/Cas9系统是一个令人兴奋的，相对较新的系统，已被证明适用于各种生物系统。 多学科的实验室工作是由一个才华横溢的本科生和研究生团队进行的，他们使用溶液相，固相和生物技术的组合。因此，从我的实验室监督的受训人员为各种职业做好了充分的准备，包括专注于化学和科学的职业。