Bioactive filtrate recovery from autothermal thermophilic aerobic digestion (ATAD) to produce microbial-derived plant stimulants

从自热嗜热需氧消化 (ATAD) 中回收生物活性滤液以生产微生物来源的植物兴奋剂

• 批准号: 531139-2018
• 负责人: Eskicioglu, Cigdem
• 金额: $ 1.82万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=650296
• 关键词: Bioactive; filtrate; recovery; autothermal; thermophilic

TerraBioGen, previously known as International Bio Recovery Corporation, is an agricultural biotech company**that develops cell-free, bio-stimulants and microbial metabolite bio-products that encourage plant growth and**enhance their tolerance to biotic and abiotic stresses. In the past, TerraBioGen operated an autothermal**thermophilic aerobic digestion (ATAD) facility in which it processed organic municipal solid waste (mostly**food waste) from the City of Vancouver and produced Class A biosolids fertilizer. The large-scale (i.e., six 60**ton and three 120 ton) digesters operated by TerraBioGen were able to produce bioactive fractions successful at**achieving ATAD conditions. These bioactive fractions contained naturally occurring bacterial strains that also**had promising effects on plant growth, such as enhanced root development, improved plant health, increased**tolerance against biotic and abiotic stresses and enriched crop yield. Since termination of the large-scale**ATAD, TerraBioGen tried to produce similar bioactive fractions using small-scale digesters (i.e., 1500, 100,**and 10 L) but was unsuccessful. Hence, TerraBioGen has an urgent need of developing similar biostimulants in**lab-scale digesters to maintain production at high level of quality. This project aims to explore the basic steps**involved in developing process and operating conditions at a lab-scale ATAD that encourages the microbial**community observed at large-scale. Laboratory experiments will be conducted at the Bioreactor Technology**Group Laboratory. ATAD will be simulated by automated fermenters with dissolved oxygen (DO), pH and**temperature probes. As substrate, food waste from cafeterias on UBC's Okanagan Campus will be used. If**results from the lab-scale experiments are promising, TerraBioGen will be able to generate bioactive fractions**and save significant capital and operational cost related to building and operating large scale ATAD digesters.**By using the biostimulant, the stress on current agricultural practices for farming more land and more irrigation**and using more fertilizer to meet increasing demand will be reduced. The project will train highly qualified**personnel in areas of waste reduction and resource recovery.

TerraBioGen前身为国际生物回收公司，是一家农业生物技术公司**，开发无细胞、生物刺激剂和微生物代谢物生物制品，促进植物生长，**增强植物对生物和非生物压力的耐受性。过去，TerraBioGen运营着一家自热**高温好氧消化(ATAD)设施，在该设施中处理来自温哥华市的有机城市固体废物(主要是**食物垃圾)，并生产A级生物固体肥料。TerraBioGen运营的大型消化池(即6个60**吨和3个120吨)能够在**达到ATAD条件下成功地生产生物活性馏分。这些生物活性部分含有自然产生的细菌菌株，这些菌株对植物生长也有很好的效果，如促进根的发育，改善植物健康，增强对生物和非生物胁迫的耐受性，并增加作物产量。自大规模ATAD终止以来，TerraBioGen曾尝试使用小型消化器(即1500、100、**和10 L)生产类似的生物活性组份，但未获成功。因此，TerraBioGen迫切需要在**实验室规模的沼气池中开发类似的生物刺激剂，以保持高质量的生产。该项目旨在探索在实验室规模的ATAD中开发过程和操作条件所涉及的基本步骤**，以鼓励大规模观察到的微生物群落。实验室实验将在生物反应器技术**小组实验室进行。ATAD将由带有溶解氧(DO)、pH和**温度探头的自动发酵罐模拟。作为基质，UBC奥卡纳根校区自助餐厅的食物垃圾将被使用。如果**实验室规模的实验结果是有希望的，TerraBioGen将能够产生生物活性成分**，并节省与建造和运营大规模ATAD消化器相关的大量资本和运营成本。**通过使用生物刺激剂，当前农业实践中耕种更多土地和更多灌溉**以及使用更多肥料以满足日益增长的需求的压力将会减轻。该项目将在减少废物和资源回收领域培训高素质的**人员。