Bioactive filtrate recovery from autothermal thermophilic aerobic digestion (ATAD) to produce microbial-derived plant stimulants

TerraBioGen，以前称为国际生物恢复公司，是一家农业生物技术公司 **，开发无细胞，生物刺激剂和微生物代谢物生物产品，促进植物生长，** 增强其对生物和非生物胁迫的耐受性。在过去，TerraBioGen经营一个自热 ** 嗜热好氧消化（ATAD）设施，其中它处理有机城市固体废物（主要是 ** 食物垃圾）从温哥华市和生产A级生物固体肥料。大规模（即，由TerraBioGen操作六个60吨和三个120吨的消化器能够成功地生产出达到ATAD条件的生物活性级分。这些生物活性组分含有天然存在的细菌菌株，这些菌株对植物生长也有很好的影响，例如增强根系发育，改善植物健康，提高对生物和非生物胁迫的耐受性以及提高作物产量。自从大规模 **ATAD终止以来，TerraBioGen试图使用小规模消化器生产类似的生物活性级分（即，1500、100、** 和10 L），但未成功。因此，TerraBioGen迫切需要在 ** 实验室规模的消化器中开发类似的生物刺激剂，以保持高质量水平的生产。本项目旨在探索在实验室规模的ATAD中开发过程和操作条件所涉及的基本步骤 **，以促进大规模观察到的微生物 ** 群落。实验室实验将在生物反应器技术 ** 集团实验室进行。ATAD将通过带有溶解氧（DO）、pH和 ** 温度探头的自动发酵罐进行模拟。作为基质，将使用UBC Okanagan校区的食堂的食物垃圾。如果 ** 实验室规模实验的结果是有希望的，TerraBioGen将能够产生生物活性组分 **，并节省与建造和运营大型ATAD消化器相关的大量资金和运营成本。通过使用生物刺激剂，将减少对目前农业实践的压力，即耕种更多的土地和更多的灌溉 **，并使用更多的肥料来满足日益增长的需求。该项目将培训减少废物和资源回收领域的高素质 ** 人员。

TerraBioGen, previously known as International Bio Recovery Corporation, is an agricultural biotech company**that develops cell-free, bio-stimulants and microbial metabolite bio-products that encourage plant growth and**enhance their tolerance to biotic and abiotic stresses. In the past, TerraBioGen operated an autothermal**thermophilic aerobic digestion (ATAD) facility in which it processed organic municipal solid waste (mostly**food waste) from the City of Vancouver and produced Class A biosolids fertilizer. The large-scale (i.e., six 60**ton and three 120 ton) digesters operated by TerraBioGen were able to produce bioactive fractions successful at**achieving ATAD conditions. These bioactive fractions contained naturally occurring bacterial strains that also**had promising effects on plant growth, such as enhanced root development, improved plant health, increased**tolerance against biotic and abiotic stresses and enriched crop yield. Since termination of the large-scale**ATAD, TerraBioGen tried to produce similar bioactive fractions using small-scale digesters (i.e., 1500, 100,**and 10 L) but was unsuccessful. Hence, TerraBioGen has an urgent need of developing similar biostimulants in**lab-scale digesters to maintain production at high level of quality. This project aims to explore the basic steps**involved in developing process and operating conditions at a lab-scale ATAD that encourages the microbial**community observed at large-scale. Laboratory experiments will be conducted at the Bioreactor Technology**Group Laboratory. ATAD will be simulated by automated fermenters with dissolved oxygen (DO), pH and**temperature probes. As substrate, food waste from cafeterias on UBC's Okanagan Campus will be used. If**results from the lab-scale experiments are promising, TerraBioGen will be able to generate bioactive fractions**and save significant capital and operational cost related to building and operating large scale ATAD digesters.**By using the biostimulant, the stress on current agricultural practices for farming more land and more irrigation**and using more fertilizer to meet increasing demand will be reduced. The project will train highly qualified**personnel in areas of waste reduction and resource recovery.