Mechanisms and implications of two-component signal transduction in Campylobacter jejuni

空肠弯曲菌双组分信号转导的机制和意义

• 批准号: 386667-2012
• 负责人: Gaynor, Erin
• 金额: $ 2.91万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=651645
• 关键词: Mechanisms; implications; two; component; signal

The ability of bacteria to sense and respond to different conditions is essential to their survival. A conserved means to enact this is the two-component signal transduction system (TCS). In typical two-component systems, a sensor protein on the bacterial surface senses an external signal and activates a regulator protein in the cytoplasm. The regulator then binds DNA to alter gene expression, which in turn alters bacterial behavior in response to the signal.****The organism we study, Campylobacter jejuni, is a prevalent enteric pathogen that lives optimally inside commensal animal hosts such as chickens. However, it can also survive (and often thrive) in unfavorable environments such as water and food processing plants, in the dirt, and on poultry surfaces in the grocery store, as well as within susceptible human hosts. Paradoxically, the percentage of the C. jejuni genome devoted to regulatory genes such as TCSs is much smaller than that of other enteric bacteria like E. coli and more similar to the human stomach-restricted Helicobacter pylori. ****These observations led us to hypothesize that critical TCSs in C. jejuni will control multiple aspects of the C. jejuni lifestyle. Indeed, we have found that the TCSs that form the basis of this research program are required for survival in chickens and also modulate key aspects of bacterial physiology. The goal of this research program is to identify which genes these systems influence both directly and indirectly, explore other bacterial factors that may interact with these systems, and reveal signals to which the systems respond. This will provide insight into important mechanisms governing C. jejuni behavior and provide models for other organisms which may likewise need to traverse diverse niches using limited regulatory machinery. ********

细菌感知和响应不同条件的能力对它们的生存至关重要。一个保守的手段来制定这是双组分信号转导系统（TCS）。 在典型的双组分系统中，细菌表面的传感器蛋白感知外部信号并激活细胞质中的调节蛋白。然后，调节因子结合DNA以改变基因表达，这反过来又改变了细菌对信号的反应。我们研究的生物体空肠弯曲菌是一种流行的肠道病原体，最适合在肠道动物宿主（如鸡）中生存。然而，它也可以在不利的环境中生存（并且经常茁壮成长），例如水和食品加工厂，污垢，杂货店的家禽表面以及易感的人类宿主。巧合的是，C.空肠肠杆菌基因组中含有调控基因，如TCS，比其他肠道细菌如E.大肠杆菌，更类似于人类胃限制性幽门螺杆菌。* 这些观察使我们假设C. jejuni将控制C.空肠的生活方式。事实上，我们已经发现，形成本研究计划基础的TCS是鸡生存所必需的，也调节细菌生理学的关键方面。该研究计划的目标是确定这些系统直接和间接影响哪些基因，探索可能与这些系统相互作用的其他细菌因子，并揭示系统响应的信号。这将提供深入了解的重要机制，治理C。空肠的行为，并提供了其他生物体的模式，可能同样需要穿越不同的小生境使用有限的监管机制。 ********