Establishing value/utility of thyroid hormone response as a biomarker for prediction of the resilience of swine following PRRSV infection

确定甲状腺激素反应作为生物标志物的价值/效用，用于预测猪感染 PRRSV 后的恢复能力

• 批准号: 529583-2018
• 负责人: Harding, John
• 金额: $ 0.12万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=653050
• 关键词: Establishing; value; utility; thyroid; hormone

Porcine reproductive and respiratory syndrome (PRRS) is one of the most important diseases of pig production worldwide. Infection during pregnancy results in abortion and reduced litter size and quality, whereas infection after birth results in respiratory disease and immune suppression. Thus, the disease has a major impact on health, production, pig welfare and environmental footprint (more sows are required) in the Canadian pork industry. The PRRS virus evolves rapidly, similar to influenza virus, making vaccination less than 100% effective. Even vaccinated animals are prone to reinfection by different strains of virus. Producers can undertake costly procedures to eliminate the virus from their farms, but farms often become re-infected within 1-2 years. For these reasons, alternative methods of control are justified. The overarching objective of this research is to discover biomarkers measured in the blood that will predict resilience to PRRS. If genetically linked, it may be possible to select animals that have increased resilience to PRRS. Two biomarkers will be investigated using sera collected from past animal experiments conducted by researchers in Canada and the USA. Use of archived samples reduces the cost and increases the speed at which it can be completed. In Phase 1, sponsored by Ontario Pork, soluble CD163 will be measured in the serum of approximately 2,200 animals across various age groups. Soluble CD163 is a marker of inflammation and the requisite receptor used by PRRS virus to replicate in an animal's immune cells. In Phase 2, sponsored by NSERC, thyroxin hormone levels will be measured in the serum of approximately 4,650 pigs. These include the same samples as used for phase 1 plus additional cohorts to enable three genome wide association studies (GWAS). The thyroxin hormones have many functions related to regulation of appetite, metabolism, growth and fetal development. If either marker is heritable, our results will significantly improve the robustness of the Canadian swine herd, thereby improving industry competitiveness, productivity and welfare.**

猪繁殖与呼吸综合征（PRRS）是世界范围内危害养猪业的重要疾病之一。怀孕期间感染会导致流产和减少产仔数和质量，而出生后感染会导致呼吸道疾病和免疫抑制。因此，该疾病对加拿大猪肉行业的健康、生产、猪福利和环境足迹（需要更多母猪）产生重大影响。PRRS病毒进化迅速，类似于流感病毒，使疫苗接种不到100%有效。即使接种疫苗的动物也容易被不同的病毒株再次感染。生产者可以采取昂贵的程序来消除农场中的病毒，但农场通常会在1-2年内再次感染。出于这些原因，替代控制方法是合理的。这项研究的首要目标是发现血液中测量的生物标志物，这些生物标志物将预测PRRS的恢复力。 如果有基因联系，就有可能选择对PRRS具有更强适应力的动物。将使用从加拿大和美国研究人员过去进行的动物实验中收集的血清研究两种生物标志物。使用存档的样本可以降低成本并提高完成的速度。在由安大略猪肉公司申办的第1阶段中，将在不同年龄组的约2，200只动物的血清中测定可溶性CD 163。可溶性CD 163是炎症的标志物，也是PRRS病毒在动物免疫细胞中复制所必需的受体。在NSERC申办的第2阶段，将测量约4，650头猪血清中的甲状腺素激素水平。这些包括与用于I期的相同的样本加上额外的队列，以实现三个全基因组关联研究（GWAS）。甲状腺激素具有调节食欲、代谢、生长和胎儿发育的多种功能。 如果任何一个标记是可遗传的，我们的研究结果将显着提高加拿大猪群的健壮性，从而提高行业竞争力，生产力和福利。