The control of mitochondrial DNA copy number

线粒体DNA拷贝数的控制

• 批准号: 386351-2012
• 负责人: Lemire, Bernard
• 金额: $ 2.04万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657194
• 关键词: control; mitochondrial; DNA; copy; number

Mitochondria are the cell's energy factory. Mitochondria produce energy in the form of ATP required to perform work such as muscle contraction or nerve cell signaling. The ATP generated in**mitochondria is made by a complex set of proteins. The genes that encode these proteins can be found in two different DNAs, nuclear DNA and mitochondrial DNA. Mitochondrial DNA is present in many copies per cell and the copy number is regulated in response to energy needs and to the environment. **This work uses a soil-living nematode (worm) to identify the genes that control the amount of mitochondrial DNA present in each cell. The nematode is easy to grow and manipulate and offers many important advantages over studying more complex organisms. However, the nematode mitochondrial DNA is extremely similar in structure and function to that of humans. Determining how mitochondrial DNA copy number is regulated is an important step in understanding how organisms generate the energy they need for growth, development and reproduction.

线粒体是细胞的能量工厂。线粒体以ATP的形式产生能量，以完成肌肉收缩或神经细胞信号传递等工作。线粒体中产生的ATP是由一组复杂的蛋白质合成的。编码这些蛋白质的基因可以在两种不同的DNA中找到，核DNA和线粒体DNA。线粒体DNA在每个细胞中存在许多拷贝，拷贝数受到能量需求和环境的调节。**这项工作使用一种土壤生活的线虫（蠕虫）来识别控制每个细胞中存在的线粒体DNA数量的基因。线虫易于生长和操作，与研究更复杂的生物体相比，它提供了许多重要的优势。然而，线虫的线粒体DNA在结构和功能上与人类极其相似。确定线粒体DNA拷贝数是如何调控的，是理解生物体如何产生生长、发育和繁殖所需能量的重要一步。