UBC Okanagan Peptide Detection System

UBC Okanagan 肽检测系统

• 批准号: RTI-2019-00421
• 负责人: Little, Jonathan
• 金额: $ 8.77万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657726
• 关键词: UBC; Okanagan; Peptide; Detection; System

The long-term objectives of my NSERC research program are to understand the interaction between metabolism and inflammation in humans. Using an integrative physiology approach where exercise and nutritional interventions are used to alter metabolism and study the resultant effects on hormones, cytokines, metabolites and immune cell function, we advance understanding of the mechanisms underlying how metabolism and inflammation are integrated. The short-term objectives of my accompanying NSERC Discovery Grant renewal are:***1) To determine how exercise intensity and pattern impact anti-inflammatory cytokine function, and the mechanisms involved ***2) To determine how nutritional ketosis impacts immune cell phenotype and function ***Specific, valid, and reliable assessment of hormones and cytokines are clearly essential for my research as they are measured in every study, typically as the major outcomes. Several other NSERC-funded researchers at our institution also need these capabilities, including collaborators Dr. A. Klegeris (who studies immunological aspects of neuron-glial interactions) and Dr. Sanjoy Ghosh (who researches the impact of dietary fatty acids on inflammation and metabolism). Their work requires high-sensitivity peptide detection from various sample types often with limited volumes (e.g., cell culture supernatants, cell/tissue lysates, and mouse plasma). The Meso Scale Discovery (MSD) MESO Quickplex SQ20 platform and the establishment of the UBC Okanagan Peptide Detection System is needed to provide our research programs with the most cutting-edge, highly sensitive, and specific technology for detecting peptides (hormones and cytokines) in biological samples.***This new research instrument will enable our research teams to measure circulating cytokines and hormones at an increased sensitivity of ~3 to 30-fold higher than current systems. This is essential because many cytokines (small peptides that are released from cells and function as inflammatory communicators) are present in very low concentrations in the blood. In order to understand how exercise and nutritional manipulations influence these cytokines a high-sensitivity assay detection system is needed to be able to measure them. Over 20 highly qualified personnel from several labs will use the equipment regularly gaining valuable biochemical assay experience with application to human physiology, metabolism, and immunology. Enhancing the ability to measure circulating cytokines and hormones with this new research instrument will yield new insights into the fundamental integration between metabolism and inflammation in humans.

我的NSERC研究计划的长期目标是了解人类代谢和炎症之间的相互作用。使用综合生理学方法，其中运动和营养干预用于改变代谢并研究对激素，细胞因子，代谢物和免疫细胞功能的影响，我们推进了对代谢和炎症如何整合的机制的理解。我伴随的NSERC发现补助金更新的短期目标是：*1）确定运动强度和模式如何影响抗炎细胞因子功能，以及相关机制 *2）确定营养酮症如何影响免疫细胞表型和功能 * 对激素和细胞因子的特异性，有效性和可靠性评估显然对我的研究至关重要，因为它们在每项研究中都被测量，通常作为主要结果。我们机构的其他几位NSERC资助的研究人员也需要这些能力，包括合作者A。Klegeris（研究神经元-神经胶质相互作用的免疫学方面）和Sanjoy Ghosh博士（研究膳食脂肪酸对炎症和代谢的影响）。他们的工作需要从各种样品类型中进行高灵敏度的肽检测，这些样品类型通常具有有限的体积（例如，细胞培养上清液、细胞/组织裂解物和小鼠血浆）。Meso Scale Discovery（MSD）MESO Quickplex SQ 20平台和UBC Okanagan肽检测系统的建立，为我们的研究项目提供了最先进、最灵敏、最特异的技术，用于检测生物样品中的肽（激素和细胞因子）。这种新的研究仪器将使我们的研究团队能够测量循环细胞因子和激素，其灵敏度比现有系统高3至30倍。这是必不可少的，因为许多细胞因子（从细胞中释放并作为炎症传播者的小肽）在血液中的浓度非常低。为了了解运动和营养操作如何影响这些细胞因子，需要高灵敏度的测定检测系统来测量它们。来自多个实验室的20多名高素质人员将定期使用该设备，获得应用于人体生理学，代谢和免疫学的宝贵生化分析经验。用这种新的研究仪器增强测量循环细胞因子和激素的能力，将对人类代谢和炎症之间的基本整合产生新的见解。