New anti-biofilm formulations and products

新的抗生物膜配方和产品

• 批准号: 532621-2018
• 负责人: Liu, Song
• 金额: $ 1.82万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657811
• 关键词: New; anti; biofilm; formulations; products

Biofilm formation on medical devices can cause persistent infections. The main feature of biofilms is their**self-produced extracellular polymeric substances (EPSs) that act as a protective shield to prevent biocide**penetration. Kane Biotech Inc. has been working on biofilm removal and dispersal. Kane Biotech has a**proprietary enzyme that can be used to break down and disperse biofilms. They are interested in developing**new products with higher potency in removing biofilms in both industrial and healthcare sectors. My research**group has successfully used Iron oxide magnetic nanoparticles (MNPs), synthesized through a simple process,**as "shield breakers" to negatively impact the integrity of biofilm EPS and cause biofilm detachment. The**Methicillin-resistant Staphylococcus aureus (MRSA) strain was used to demonstrate the efficacy of MNPs. The**highest logarithmic reduction (4.71 log) of MRSA biofilm was achieved after the treatment with 30 mg/mL of**11 nm MNPs under rotating magnetic field for 15 minutes. MNPs caused strong mechanical disruption to the**matrix and led to biofilm dispersal. Both alternating and rotating magnetic fields improved the effect of MNPs**on biofilm damage. The increased MNPs solution temperature caused by the hyperthermic effect in the AC**field led to biofilm dispersal while the MNPs caused physical damage on biofilms in a rotating magnetic field.**We hypothesize that combination of the MNPs synthesized in our lab with Kane Biotech's proprietary**anti-biofilm enzyme will lead to new formulations and products with synergistic anti-biofilm activity. That can**be translated to new anti-biofilm products that Kane can commercialize.

医疗器械上的生物膜形成可导致持续性感染。生物膜的主要特征是它们自身产生的细胞外聚合物（EPS），其充当保护屏障以防止杀生物剂渗透。凯恩生物技术公司一直致力于生物膜的去除和扩散。凯恩生物技术公司有一种专利酶，可以用来分解和分散生物膜。他们有兴趣开发在工业和医疗保健领域具有更高去除生物膜效力的新产品。我的研究小组成功地使用了氧化铁磁性纳米颗粒（MNP），通过一个简单的过程合成，** 作为“屏蔽断路器”，对生物膜EPS的完整性产生负面影响，并导致生物膜脱离。** 耐甲氧西林金黄色葡萄球菌（MRSA）菌株用于证明MNP的功效。在旋转磁场下用30 mg/mL **11 nm MNP处理15分钟后，实现MRSA生物膜的 ** 最高对数减少（4.71 log）。MNP对 ** 基质造成强烈的机械破坏，并导致生物膜分散。交变磁场和旋转磁场都改善了MNP ** 对生物膜损伤的影响。在AC** 场中由过热效应引起的MNP溶液温度升高导致生物膜分散，而MNP在旋转磁场中对生物膜造成物理损伤。我们假设，我们实验室合成的MNP与凯恩生物技术公司专有的 ** 抗生物膜酶的组合将产生具有协同抗生物膜活性的新制剂和产品。这可以转化为凯恩可以商业化的新的抗生物膜产品。