An Ultrasensitive Microfluidic Biosensor Based on Vertically-Aligned MoS2 Nanolayers

基于垂直排列 MoS2 纳米层的超灵敏微流控生物传感器

• 批准号: 494012-2016
• 负责人: Liu, Xinyu
• 金额: $ 12.29万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657826
• 关键词: Ultrasensitive; Microfluidic; Biosensor; Based; Vertically

Next-generation biosensors that exploit highly-sensitive, two-dimensional (2D) nanomaterials could provide superior analytical performance and thus enable rapid, ultrasensitive tests for a variety of applications in areas such as molecular diagnosis, environmental monitoring, and food safety inspection. Atomically-thin monolayer molybdenum disulfide (MoS2), as an emerging graphene-like 2D nanomaterial, has attracted significant attention from the biosensor community. Despite the recent advancement in developing high-performance monolayer MoS2 biosensors, previously reported devices suffer from low fabrication yield, challenging biofunctionalization, and low reproducibility; these issues can be largely attributed to some inherent property and preparation process of monolayer MoS2 that are not favorable in biosensor development. In this project, we propose, for the first time, to integrate a novel form of MoS2 nanostructure, vertically-aligned MoS2 nanolayers (VAMNs), into microfluidic field-effect-transistor (FET) biosensors. Because of its unique nanostructures, the VAMNs enable a few new features that do not exist in previous MoS2 biosensors, including facile and reliable covalent immobilization of biomolecules on its vertically-aligned, populated active edge sites and good computability with batch microfabrication. In the meanwhile, the proposed FET-biosensor will also retain the advantageous physical properties of monolayer MoS2 such as its direct bandgap, ultrahigh sensitivity to surface molecule binding, and fast response; therefore, it could provide significantly-enhanced biosensing performance including ultrahigh sensitivity and high stability/reproducibility. The microfluidic design of our biosensor also allows easy device operation, multiplexed assay, and low sample consumption. As the first demonstration, we will apply our FET-biosensor to rapid, ultrasensitive detection of brain injury protein markers for constant monitoring of patient brain conditions during cardiac surgery. This research will offer a powerful VAMN-based biosensing platform, which could find many important applications where ultrahigh sensitivity and very short array time are desired.

利用高灵敏度二维（2D）纳米材料的下一代生物传感器可以提供上级分析性能，从而为分子诊断、环境监测和食品安全检查等领域的各种应用提供快速、超灵敏的测试。 原子薄单层二硫化钼（MoS 2）作为一种新兴的石墨烯类二维纳米材料，引起了生物传感器界的极大关注。 尽管最近在开发高性能单层MoS 2生物传感器方面取得了进展，但先前报道的器件具有低制造产率，具有挑战性的生物功能化和低再现性;这些问题可以在很大程度上归因于单层MoS 2的一些固有性质和制备过程，这些在生物传感器开发中并不有利。 在这个项目中，我们首次提出将一种新型的MoS 2纳米结构（垂直排列的MoS 2纳米层（VAMN））集成到微流体场效应晶体管（FET）生物传感器中。 由于其独特的纳米结构，VAMNs实现了一些以前的MoS 2生物传感器中不存在的新功能，包括生物分子在其垂直对齐的填充活性边缘位点上的简单可靠的共价固定以及批量微制造的良好可计算性。 同时，所提出的FET-生物传感器还将保留单层MoS 2的有利物理性质，例如其直接带隙、对表面分子结合的超灵敏度和快速响应;因此，它可以提供显著增强的生物传感性能，包括超灵敏度和高稳定性/再现性。 我们的生物传感器的微流体设计还允许容易的装置操作、多重测定和低样品消耗。 作为第一个演示，我们将应用我们的FET生物传感器快速，超灵敏地检测脑损伤蛋白标志物，以便在心脏手术期间持续监测患者的大脑状况。 这项研究将提供一个强大的基于VAMN的生物传感平台，它可以找到许多重要的应用，其中需要灵敏度和非常短的阵列时间。