Novel microfluidic electrochemical biosensor for rapid diagnosis of biomarkers

用于快速诊断生物标志物的新型微流控电化学生物传感器

• 批准号: 515726-2017
• 负责人: SanatiNezhad, Amir
• 金额: $ 1.82万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=640622
• 关键词: Novel; microfluidic; electrochemical; biosensor; rapid

Immunosensors are very known among different types of biosensors for rapid diagnosis of biomarkers in bodilyfluids. The label-format of immunosensors allows for flexible implementation of biosensing in a wide range ofdetection schemes. However, these systems are limited in very high sensitive detection of minutes of moleculesin complex biological samples. There is also a trade-off in the time, cost and additional steps associated withincorporating reporter molecules into the label-based process. Moreover, the use of optical detection systems inpoint-of-care devices has one more constraint in detection of multiple biomarkers simultaneously in a singlemultiplexed biosensor as they require an external light source, optical transducer and other optical componentslike lenses and reflectors, which makes the detection system bulky and complex. Among various types oflabel-free detection techniques, the electrochemical sensing is the simplest to integrate, highly sensitive andmore compatible with multiplexing and integration into microfluidics, which makes it ideal for point-of-carediagnosis.In this work, we will develop an integrated and autonomous microfluidic-based biosensor for rapid detection ofbrain injury biomarkers. A new bonding technology will be developed to adhere electrodes to microfluidiclayers noninvasively to enable efficient performance of electrodes and point-of-care detection of biomarkers. Ifsuccessful, the microfluidic nano-biosensor proposed in this work will be used for rapid diagnosis of threedifferent biomarkers of brain injury in blood samples of patients in less than an hour. The expertise of theapplicant in microfluidics and biosensors ensures the success of this project.Sensoreal Inc. will benefit from label-free biosensors to extend the application of their technology for rapid andautomated detection of multiple biomarkers in human biological samples using a point-of-care diagnostictechnology. The lower cost and predictive point of care sensors will enable the rapid and large scale diseasescreening and facilitate monitoring the efficacy of therapeutics, therefore benefit the health system in Canada.

免疫传感器在不同类型的生物传感器中非常有名，用于快速诊断体液中的生物标志物。免疫传感器的标签格式允许在广泛的检测方案中灵活地实施生物传感。然而，这些系统在复杂生物样品中几分钟分子的高灵敏度检测方面受到限制。在将报告分子纳入基于标签的过程中，还需要在时间、成本和额外步骤上进行权衡。此外，在护理点设备中使用光学检测系统在单个多路生物传感器中同时检测多个生物标志物时存在另一个限制，因为它们需要外部光源、光学传感器和其他光学元件，如透镜和反射器，这使得检测系统体积庞大且复杂。在各种类型的无标签检测技术中，电化学传感是最容易集成的，高灵敏度，更兼容多路复用和集成到微流体中，这使其成为护理点诊断的理想选择。在这项工作中，我们将开发一种集成的、自主的基于微流体的生物传感器，用于快速检测脑损伤生物标志物。将开发一种新的粘接技术，将电极无创地粘接在微流体层上，以实现电极的高效性能和生物标志物的即时检测。如果成功，这项工作中提出的微流控纳米生物传感器将用于在不到一小时内快速诊断患者血液样本中脑损伤的三种不同生物标志物。申请人在微流体和生物传感器方面的专业知识确保了这个项目的成功。Sensoreal公司将受益于无标签生物传感器，以扩展其技术的应用，使用即时诊断技术快速自动检测人类生物样品中的多种生物标志物。低成本和预测性护理点传感器将实现快速和大规模的疾病筛查，并促进治疗效果的监测，从而使加拿大的卫生系统受益。