Diapause, Stress Tolerance and Molecular Chaperones in Artemia franciscana
卤虫的滞育、胁迫耐受性和分子伴侣
基本信息
- 批准号:RGPIN-2016-04882
- 负责人:
- 金额:$ 3.93万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2018
- 资助国家:加拿大
- 起止时间:2018-01-01 至 2019-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Embryos of the crustacean, Artemia franciscana, develop into swimming larvae or they encyst and enter diapause, a physiological state characterized by developmental arrest and greatly reduced metabolism. Encysted embryos (cysts) are very stress tolerant, surviving extreme temperature, desiccation and years without oxygen when hydrated at ambient temperature. A. franciscana cysts therefore represent one of the most stress tolerant multicellular life forms on Earth. A. franciscana embryos exhibit differential gene regulation during diapause-destined development, producing proteins required for the initiation, maintenance and termination of diapause, with maintenance the period of greatest stress tolerance. The global objectives of the proposed research are to elucidate cell, molecular and biochemical mechanisms by which A. franciscana regulate diapause-specific development and determine how embryos survive severe physiological stress. Heat shock factor 1 (HSF1) which normally activates genes expressed during physiological stress will be tested to determine if it regulates gene expression during diapause. Of particular interest is an abundant, diapause-specific molecular chaperone, p26, which functions in the absence of an energy supply. p26 is thought to bind denaturing proteins in diapause embryos and protect them from irreversible denaturation. Consequently, substrate proteins of p26 will be identified. Several molecular chaperones such as Hsp40, Hsp70 and Hsp90 require energy for activity. The synthesis of these proteins will be investigated during diapause-destined embryo development, as will their protective function when energy is limiting, as occurs in diapause. Proteins that regulate diapause termination will also be identified. Thus, a central question addressed by the proposed research is how embryos of A. franciscana survive severe stressors which kill most other organisms rather quickly. Are molecular chaperones responsible for stress tolerance and, if so, how do they contribute to this process? Answering these questions will provide significant fundamental insights into diapause and the functions of essential cellular proteins such as the molecular chaperones. On an applied level, the proposed research may facilitate the use of A. franciscana in aquaculture where the organism is used to feed the larvae of commercially important fish and aquatic invertebrates. Hsp70, for example, plays a role in the resistance of A. franciscana to bacterial infection, indicating that the proposed work will lead to improvements in the culture of A. franciscana. Moreover, a better understanding of diapause has consequences for agriculture, forestry and medicine as it may allow for the development of methods for the control of insect pests able to survive winter and attempts at their eradication when in diapause, this of direct benefit to Canada.
甲壳动物卤虫的胚胎发育成游泳的幼虫,或者它们包囊并进入滞育,滞育是一种以发育停滞和代谢大大减少为特征的生理状态。包被的胚胎(胞囊)具有非常强的抗压能力,在环境温度下水合时可以在极端温度、干燥和无氧条件下存活数年。A.因此,弗朗西斯卡纳孢囊代表了地球上最耐压力的多细胞生命形式之一。A. franciscana胚胎在滞育发育过程中表现出不同的基因调控,产生滞育的起始、维持和终止所需的蛋白质,其中维持期是最大的胁迫耐受期。该研究的总体目标是阐明A. franciscana调节滞育特异性发育,并决定胚胎如何在严重的生理应激下存活。热休克因子1(HSF 1),通常激活基因表达的生理压力期间将进行测试,以确定它是否调节基因表达滞育期间。特别令人感兴趣的是一个丰富的,滞育特异性分子伴侣,p26,它的功能在缺乏能量供应。p26被认为与滞育胚胎中的变性蛋白结合,并保护它们免受不可逆变性。因此,p26的底物蛋白将被鉴定。几种分子伴侣如Hsp40、Hsp70和Hsp90需要能量来进行活性。这些蛋白质的合成将在滞育胚胎发育过程中进行研究,当能量有限时,它们的保护功能也会发生在滞育中。还将鉴定调节滞育终止的蛋白质。因此,这项研究提出的一个中心问题是A.弗朗西斯卡纳在严重的压力下存活下来,而这些压力会很快杀死大多数其他生物。分子伴侣是否负责胁迫耐受性,如果是的话,它们是如何参与这一过程的?这些问题的解决将为滞育和分子伴侣等重要细胞蛋白的功能提供重要的基础性见解。在应用层面上,拟议的研究可能会促进A。franciscana在水产养殖中,该生物体用于喂养商业上重要的鱼类和水生无脊椎动物的幼虫。例如,Hsp70在A. franciscana对细菌感染的敏感性,表明所提出的工作将导致A.弗兰西斯卡纳。此外,更好地了解滞育对农业、林业和医学也有影响,因为它可能有助于制定控制能够越冬的害虫的方法,并试图在滞育期间消灭这些害虫,这对加拿大有直接的好处。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MacRae, Thomas其他文献
MacRae, Thomas的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MacRae, Thomas', 18)}}的其他基金
Diapause, Stress Tolerance and Molecular Chaperones in Artemia franciscana
卤虫的滞育、胁迫耐受性和分子伴侣
- 批准号:
RGPIN-2016-04882 - 财政年份:2017
- 资助金额:
$ 3.93万 - 项目类别:
Discovery Grants Program - Individual
Diapause, Stress Tolerance and Molecular Chaperones in Artemia franciscana
卤虫的滞育、胁迫耐受性和分子伴侣
- 批准号:
RGPIN-2016-04882 - 财政年份:2016
- 资助金额:
$ 3.93万 - 项目类别:
Discovery Grants Program - Individual
Diapause regulation and stress tolerance in artemia embryos: gene expression, protein function and metabolism
卤虫胚胎的滞育调节和应激耐受性:基因表达、蛋白质功能和代谢
- 批准号:
7661-2011 - 财政年份:2015
- 资助金额:
$ 3.93万 - 项目类别:
Discovery Grants Program - Individual
Diapause regulation and stress tolerance in artemia embryos: gene expression, protein function and metabolism
卤虫胚胎的滞育调节和应激耐受性:基因表达、蛋白质功能和代谢
- 批准号:
7661-2011 - 财政年份:2014
- 资助金额:
$ 3.93万 - 项目类别:
Discovery Grants Program - Individual
Instrumentation for Small Volume Applications in DNA, RNA and Protein Analysis
DNA、RNA 和蛋白质分析中小批量应用的仪器
- 批准号:
472382-2015 - 财政年份:2014
- 资助金额:
$ 3.93万 - 项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
Diapause regulation and stress tolerance in artemia embryos: gene expression, protein function and metabolism
卤虫胚胎的滞育调节和应激耐受性:基因表达、蛋白质功能和代谢
- 批准号:
7661-2011 - 财政年份:2013
- 资助金额:
$ 3.93万 - 项目类别:
Discovery Grants Program - Individual
Diapause regulation and stress tolerance in artemia embryos: gene expression, protein function and metabolism
卤虫胚胎的滞育调节和应激耐受性:基因表达、蛋白质功能和代谢
- 批准号:
7661-2011 - 财政年份:2012
- 资助金额:
$ 3.93万 - 项目类别:
Discovery Grants Program - Individual
Diapause regulation and stress tolerance in artemia embryos: gene expression, protein function and metabolism
卤虫胚胎的滞育调节和应激耐受性:基因表达、蛋白质功能和代谢
- 批准号:
7661-2011 - 财政年份:2011
- 资助金额:
$ 3.93万 - 项目类别:
Discovery Grants Program - Individual
Refrigerated Centrifuge for Small Volume Applications
适用于小体积应用的冷冻离心机
- 批准号:
422314-2012 - 财政年份:2011
- 资助金额:
$ 3.93万 - 项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
Incubator shaker and accessories
培养摇床及配件
- 批准号:
407542-2011 - 财政年份:2010
- 资助金额:
$ 3.93万 - 项目类别:
Research Tools and Instruments - Category 1 (<$150,000)
相似国自然基金
Tmem30a通过ER Stress/NF-κB信号通路调节肠上皮细胞屏障功能稳态介导炎症性肠病的研究
- 批准号:82300629
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
二甲双胍抗肥胖新机制:调节小胶质细胞ER stress-EVs缓解下丘脑炎症
- 批准号:
- 批准年份:2022
- 资助金额:30 万元
- 项目类别:青年科学基金项目
肿瘤相关巨噬细胞通过Stress Granule 形成调控炎症小体促进舌鳞癌转移的机制研究
- 批准号:
- 批准年份:2021
- 资助金额:10.0 万元
- 项目类别:省市级项目
ACSL4/ER stress/GPX4通路在溃疡性结肠炎中对Ferroptosis的调控机制研究
- 批准号:
- 批准年份:2021
- 资助金额:30 万元
- 项目类别:青年科学基金项目
炎症相关因子 RKIP 通过活化 ER stress 相关的IRE1α/XBP1 信号轴调控肝脏疾病的机制研究
- 批准号:LY22H030007
- 批准年份:2021
- 资助金额:0.0 万元
- 项目类别:省市级项目
糖尿病心肌病新机制:支链氨基酸(BCAA)代谢障碍通过下调冠脉内皮细胞STIM1抑制mTORC2-Akt1通路和激活ER stress-UPR导致冠脉微血管损伤
- 批准号:82000356
- 批准年份:2020
- 资助金额:24.0 万元
- 项目类别:青年科学基金项目
基于ROS-ER stress-Ca2+信号通路研究健脾益肺II号减少COPD气道上皮细胞凋亡的作用机制
- 批准号:
- 批准年份:2020
- 资助金额:55 万元
- 项目类别:面上项目
CAMKIV-MHC Class I-ER Stress途径对骨骼肌炎症及再生的调控及机制研究
- 批准号:
- 批准年份:2019
- 资助金额:10.0 万元
- 项目类别:省市级项目
舌鳞癌细胞通过ER stress传递激活巨噬细胞调控肿瘤转移的机制研究
- 批准号:
- 批准年份:2019
- 资助金额:10.0 万元
- 项目类别:省市级项目
β-arrestin-2通过ER-stress/PUMA调控Beclin1信号在结肠炎中的作用
- 批准号:81800458
- 批准年份:2018
- 资助金额:21.0 万元
- 项目类别:青年科学基金项目
相似海外基金
Antag0onistic histone modifiers coordinate flooding stress tolerance and memory in plants
拮抗组蛋白修饰剂协调植物的洪水胁迫耐受性和记忆
- 批准号:
BB/Y006062/1 - 财政年份:2024
- 资助金额:
$ 3.93万 - 项目类别:
Research Grant
Development of physiological index on the stress tolerance and the analytical method of distribution of riparian trees
河岸树木抗逆生理指标的建立及分布分析方法
- 批准号:
23K04040 - 财政年份:2023
- 资助金额:
$ 3.93万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Unlocking the epigenetic potential of stress tolerance in rice
释放水稻抗逆性的表观遗传潜力
- 批准号:
23KF0288 - 财政年份:2023
- 资助金额:
$ 3.93万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Investigation of the effects of macrolide antimicrobial agents on oxidative stress tolerance in trophoblast cells
大环内酯类抗菌药物对滋养层细胞氧化应激耐受性影响的研究
- 批准号:
23K08863 - 财政年份:2023
- 资助金额:
$ 3.93万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Enhanced Growth and Stress Tolerance in Plants After Treatment With Ethylene
乙烯处理后植物的生长和胁迫耐受性增强
- 批准号:
2233695 - 财政年份:2023
- 资助金额:
$ 3.93万 - 项目类别:
Continuing Grant
Unlocking the epigenetic potential of stress tolerance in rice
释放水稻抗逆性的表观遗传潜力
- 批准号:
23K13957 - 财政年份:2023
- 资助金额:
$ 3.93万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Power naps: ribosome hibernation in bacterial drug targeting and stress tolerance
小睡:细菌药物靶向和应激耐受中的核糖体冬眠
- 批准号:
2882641 - 财政年份:2023
- 资助金额:
$ 3.93万 - 项目类别:
Studentship
Light-based seed priming for abiotic stress tolerance of horticultural crops
用于园艺作物非生物胁迫耐受性的光种子引发
- 批准号:
2898136 - 财政年份:2023
- 资助金额:
$ 3.93万 - 项目类别:
Studentship
Importance of quantitative and qualitative control of ROS production in plant chilling stress tolerance
ROS产生的定量和定性控制在植物耐冷胁迫中的重要性
- 批准号:
23KJ1357 - 财政年份:2023
- 资助金额:
$ 3.93万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Molecular mechanisms of plant low temperature stress tolerance
植物低温胁迫耐受的分子机制
- 批准号:
RGPIN-2020-06232 - 财政年份:2022
- 资助金额:
$ 3.93万 - 项目类别:
Discovery Grants Program - Individual