Incubator shaker and accessories

该仪器将主要用于细菌（通常为大肠杆菌）的受控培养。这是提交申请的研究人员和在生物学系工作的其他人将有机会使用培养箱振荡器和配件的必要程序。培养的细菌将用于克隆编码滞育期间上调的蛋白质的cDNA，并用于表达野生型和突变的真核蛋白质，然后将其纯化。这些蛋白质用于几种体外测定，以研究蛋白质的性质，例如分子伴侣，并作为抗原用于生产用于蛋白质定量和定位的抗体。在受控温度和通风条件下，使用振荡培养箱培养卤虫。将研究卤虫胚胎发育和滞育（一种生理休眠状态和增强的胁迫耐受性）过程中的基因表达和蛋白质合成。蛋白质结构/功能的关系和分子伴侣的细胞存活滞育期间的贡献是这项工作的重要组成部分。利用振荡培养箱进行的研究将对细胞、分子和发育生物学做出基础性贡献，并有许多应用。对滞育的研究可以揭示昆虫如何抵抗压力，这在农业和林业中很重要，因为这些生物作为害虫的影响因进入滞育和在恶劣环境条件下生存的能力而加剧。对滞育的深入了解将促进卤虫作为水产养殖饲料的使用。MacRae博士研究了HSP 27（一种小的热休克蛋白）在乳腺癌细胞转移中的作用。显然，通过使用本文所寻求的培养箱振荡器和附件，有可能做出对商业和健康具有直接影响的发现。这些设备还将促进解决基本问题的经验，这些问题是NSERC支持的研究计划的基石。资助这一提案将使申请人在有效利用赠款资金和在几个生物学科进行现代实验的能力方面具有明确的优势。

The instrumentation will be used primarily for the controlled culture of bacteria, generally Escherichia coli. This is an essential procedure for the researcher submitting the application and for others working in the Department of Biology who will have access to the incubator shaker and accessories. Cultured bacteria will be used in cloning cDNAs encoding proteins up-regulated during diapause, and for expression of wild type and mutated eukaryotic proteins which are then purified. These proteins are used in several in vitro assays to investigate the properties of proteins such as molecular chaperones and as antigens for production of antibodies used in protein quantification and localization. The incubator shaker will be used to grow Artemia under controlled temperature and aeration. Gene expression and protein synthesis during Artemia embryo development and diapause, a state of physiological dormancy and enhanced stress tolerance, will be studied. Protein structure/function relationships and the contribution of molecular chaperones to cell survival during diapause are important components of this work. The research employing the incubator shaker will make fundamental contributions to cell, molecular and developmental biology, and there are many applications of the work. Examination of diapause may reveal how insects resist stress, this of importance in agriculture and forestry where the impact of these organisms as pests is exacerbated by the ability to enter diapause and survive harsh environmental conditions. A greater understanding of diapause will enhance the use of Artemia as feed in aquaculture. Dr. MacRae studies the role of HSP27, a small heat shock protein, in breast cancer cell metastasis. Clearly there is potential to make discoveries which have direct consequences to commerce and health through use of the incubator shaker and accessories sought herein. The equipment will also facilitate experiemnts addressing basic questions which are the cornerstone of research programs supported by NSERC. Funding this proposal will put the applicant at a definite advantage in the effective use of grant funds and the ability to conduct modern experimentation in several biological disciplines.