Instrumentation for Small Volume Applications in DNA, RNA and Protein Analysis

DNA、RNA 和蛋白质分析中小批量应用的仪器

• 批准号: 472382-2015
• 负责人: MacRae, Thomas
• 金额: $ 1.82万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564735
• 关键词: Instrumentation; Small; Volume; Applications; DNA

The instrumentation requested, a Nanodrop spectrophotometer and a gradient PCR machine, will be employed together for the analysis of DNA, RNA and proteins which are generally expensive to prepare and often available for experimentation in very small volumes. Uses for the equipment, which encompass the generation and quantitation of cDNAs for cloning and the synthesis of dsRNA for RNA interference, are routine, but essential to the applicants and their trainees. Projects benefiting from the funding include analysis of gene expression and protein synthesis during crustacean embryo diapause, a state of physiological dormancy and enhanced stress tolerance. Molecular chaperones, proteins that protect other proteins during exposure of cells to stress such as heat, will be examined. Effects on activity, abundance, trafficking and localization of plant cell proteins by post-translationally added ubiquitin molecules will be examined. Protein modification by addition of ubiquitins mediates plant growth and development, defense against pathogen attack and resistance to abiotic stress. The instrumentation will therefore be used to study fundamental aspects of cell, molecular and developmental biology. Applications of the research are many. Examination of diapause will enhance the use of Artemia as feed in aquaculture and aid in the eradication of insect pests able to undergo diapause. How the molecular chaperone Hsp70 augments resistance to bacterial diseases in aquatic animals, many of which are used in aquaculture, will be studied as will the role of ubiquitins in plant disease. Through the use of the requested equipment there is potential to make discoveries with direct consequences for commerce and health. The equipment will also facilitate experiments addressing basic research questions, the cornerstone of NSERC supported research. Funding this modest proposal will put the applicants at a definite advantage in the effective use of grant funds and the ability to conduct modern biological experiments.

所要求的仪器，一个Nanodrop分光光度计和一个梯度PCR机，将一起用于DNA，RNA和蛋白质的分析，这通常是昂贵的准备和经常可用于实验在非常小的体积。该设备的用途包括用于克隆的cDNA的产生和定量以及用于RNA干扰的dsRNA的合成，这些用途是常规的，但对于申请人及其受训者是必不可少的。 受益于该基金的项目包括分析甲壳类动物胚胎滞育期间的基因表达和蛋白质合成，滞育是一种生理休眠状态和增强的胁迫耐受性。分子伴侣，蛋白质，保护其他蛋白质在细胞暴露于压力，如热，将被检查。将研究翻译后添加的泛蛋白分子对植物细胞蛋白的活性、丰度、运输和定位的影响。通过添加泛素修饰蛋白质介导植物生长发育、防御病原体攻击和抵抗非生物胁迫。因此，仪器将用于研究细胞，分子和发育生物学的基本方面。研究的应用很多。滞育的检查将促进卤虫作为水产养殖饲料的使用，并有助于消灭能够滞育的害虫。分子伴侣Hsp70如何增强水生动物对细菌性疾病的抵抗力，其中许多用于水产养殖，将研究泛素在植物疾病中的作用。通过使用所要求的设备，有可能发现对商业和健康有直接影响的问题。这些设备还将促进解决基础研究问题的实验，这是NSERC支持研究的基石。资助这一适度的建议将使申请人在有效利用赠款资金和进行现代生物实验的能力方面处于明显的优势。