Genetic dissection of the NADPH metabolic network in drosophila

果蝇 NADPH 代谢网络的遗传解剖

• 批准号: RGPIN-2018-05551
• 负责人: Merritt, Thomas
• 金额: $ 3.64万
• 依托单位: Laurentian University of Sudbury
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=659650
• 关键词: Genetic; dissection; NADPH; metabolic; network

All organisms, from microbes to flies to people, have physical characteristics, their phenotypes, that are in part a function of their genetic material, their genotype. These phenotypes are what distinguish one organism from the next and can be as innocuous as eye color or as biologically crucial as basic metabolism or response to a particular drug. Modern science is very good at determining genotype, reading an organism's genetic material, but still working on connecting genotype to phenotype. My research seeks to better explain this connection in biological networks – using the interacting genes and proteins of a metabolic network as a model system. ******The Long Term Goal of my research program is to describe the path between genomic molecular diversity and complex biological phenotypes, and focus on metabolic networks to understand this connection. Specifically, we quantify the genetic and metabolic interactions that regulate a network of enzymes that reduce and oxidize the metabolic cofactor NADP(H) to define and understand biological networks in general.******Short-term Aims: I have two short term Aims for this granting period:***1) Define the metabolic and biochemical effects (phenotype) of variation across the NADP(H) network in Drosophila melanogaster***2) Identify novel factors (genes) that contribute to this NADP(H) network******Over the 5 years of this grant, these two Aims will allow us to understand the network as a whole and provide fundamental knowledge of metabolic networks in general.

所有的生物，从微生物到苍蝇到人类，都有物理特征，它们的表型，这部分是它们的遗传物质，基因型的功能。 这些表型是区分一个生物体与下一个生物体的关键，它们可以像眼睛的颜色一样无害，也可以像基本代谢或对特定药物的反应一样具有生物学意义。现代科学非常擅长确定基因型，阅读有机体的遗传物质，但仍致力于将基因型与表型联系起来。我的研究旨在更好地解释生物网络中的这种联系-使用代谢网络中相互作用的基因和蛋白质作为模型系统。** 我的研究计划的长期目标是描述基因组分子多样性和复杂生物表型之间的路径，并专注于代谢网络，以了解这种联系。具体来说，我们量化了调节还原和氧化代谢辅因子NADP（H）的酶网络的遗传和代谢相互作用，以定义和理解一般的生物网络。短期目标：我有两个短期的目标，在此期间授予：*1）定义的代谢和生化效应（表型）的变化在整个NADP（H）网络在果蝇 *2）确定新的因素（基因），有助于这个NADP（H）网络 * 在5年的资助，这两个目标将使我们能够了解网络作为一个整体，并提供一般的代谢网络的基础知识。