Genetic dissection of the NADPH metabolic network in drosophila

所有的生物，从微生物到苍蝇再到人类，都有物理特征，它们的表现型，这在一定程度上是它们的遗传物质的功能，它们的基因型。这些表型是区分不同生物的关键，它们可以像眼睛颜色一样无害，也可以像基本代谢或对特定药物的反应一样至关重要。现代科学非常擅长确定基因型，读取生物体的遗传物质，但仍在努力将基因型与表型联系起来。我的研究试图用代谢网络的相互作用基因和蛋白质作为模型系统来更好地解释生物网络中的这种联系。******我的研究计划的长期目标是描述基因组分子多样性和复杂生物表型之间的路径，并专注于代谢网络来理解这种联系。具体来说，我们量化了遗传和代谢相互作用，这些相互作用调节了一个酶网络，这些酶可以减少和氧化代谢辅助因子NADP(H)，从而定义和理解一般的生物网络。******短期目标：我在这个资助期有两个短期目标：***1)定义果蝇NADP（H)网络变异的代谢和生化效应（表型）***2）确定有助于这个NADP(H)网络的新因素（基因）******在这个资助的5年里，这两个目标将使我们能够理解整个网络，并提供代谢网络的基本知识。

All organisms, from microbes to flies to people, have physical characteristics, their phenotypes, that are in part a function of their genetic material, their genotype. These phenotypes are what distinguish one organism from the next and can be as innocuous as eye color or as biologically crucial as basic metabolism or response to a particular drug. Modern science is very good at determining genotype, reading an organism's genetic material, but still working on connecting genotype to phenotype. My research seeks to better explain this connection in biological networks using the interacting genes and proteins of a metabolic network as a model system. ******The Long Term Goal of my research program is to describe the path between genomic molecular diversity and complex biological phenotypes, and focus on metabolic networks to understand this connection. Specifically, we quantify the genetic and metabolic interactions that regulate a network of enzymes that reduce and oxidize the metabolic cofactor NADP(H) to define and understand biological networks in general.******Short-term Aims: I have two short term Aims for this granting period:***1) Define the metabolic and biochemical effects (phenotype) of variation across the NADP(H) network in Drosophila melanogaster***2) Identify novel factors (genes) that contribute to this NADP(H) network******Over the 5 years of this grant, these two Aims will allow us to understand the network as a whole and provide fundamental knowledge of metabolic networks in general.