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Market Assessment for Ultrastable Cryo-electron Microscopy Holder

超稳定冷冻电子显微镜支架的市场评估

基本信息

批准号：
531916-2018
负责人：
Sciaini, German
金额：
$ 1.09万
依托单位：
University of Waterloo
依托单位国家：
加拿大
项目类别：
Idea to Innovation
财政年份：
2018
资助国家：
加拿大
起止时间：
2018-01-01 至 2019-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=664579
关键词：
Market Assessment Ultrastable Cryo electron

项目摘要

One important technical problem in cryo-electron microscopy (cryo-EM), when trying to attain atomically resolved 3D structures of frozen hydrated specimens, is the negative effect on the resolution due to the vibration from liquid nitrogen cooling. Sample holders for cryo-EM have so far relied on the use of a Dewar with liquid nitrogen to cool down sample to temperatures below 130K. The use of liquid nitrogen is often inconvenient and difficult to implement. Water crystallization inside the Dewar and relative hot spots may induce vibration through bubbling, limiting the attainable spatial resolution. Significant user expertise is required under such adverse conditions to perform single particle cryo-EM experiments and successfully achieve atomic spatial resolution (below 0.3 nm). There is thus a need for cryo-EM holder design that is stable (minimal to no vibration) and can provide prolonged cooling periods, hence longer viewing**times.**Waterloo researchers have designed a cryo-EM holder that does not require the use of liquid nitrogen and thus avoids the constant refilling step inherent with this method and allows imaging at any arbitrary sample tilt angle with no liquid spills. The novel Waterloo holder is quasi-vibration free and provides lower temperatures than conventional cryo-EM holders based on liquid nitrogen cooling. A provisional US patent application will be filed very soon.****

冷冻电子显微镜（cryo-EM）中的一个重要技术问题是，当试图获得冷冻水合样品的原子分辨3D结构时，由于液氮冷却的振动而对分辨率产生负面影响。到目前为止，冷冻电镜的样品架依赖于使用带有液氮的杜瓦瓶将样品冷却到130 K以下。液氮的使用通常不方便且难以实施。杜瓦瓶内部的水结晶和相对热点可能会通过鼓泡引起振动，从而限制可达到的空间分辨率。在这种不利条件下，需要大量的用户专业知识来执行单粒子冷冻EM实验并成功实现原子空间分辨率（低于0.3 nm）。因此，需要稳定（振动最小到无振动）并且可以提供延长的冷却期的冷冻EM保持器设计，从而延长观看 ** 时间。** Waterloo的研究人员设计了一种cryo-EM保持器，它不需要使用液氮，因此避免了这种方法固有的恒定再填充步骤，并允许在任何任意样品倾斜角度下成像，而不会有液体溢出。新颖的滑铁卢保持器是准无振动的，并且提供比基于液氮冷却的常规低温EM保持器更低的温度。美国专利申请将很快提交 *。