State-of-the-art multi-electrode array recording system for a core facility serving twelve plus faculty members from eight departments at the University of Waterloo

多电极阵列（MEA）记录系统是一种突破性的设备，使研究人员能够同时监测 * 数百个细胞的电活动，从单细胞生物到组织细胞，* 如神经元或心肌细胞。 MEA设备是测量单细胞真核生物电活动所必需的，这在传统微电极记录中是不可访问的。 ** Spafford实验室 * 克隆并表达了来自 * 单细胞真核生物的第一个电压门控钠和钙通道，并在2018年夏天使用Axion*Biosystems Maestro Edge和Automate Scientific MED 64 Presto提供的MEA演示装置成功测量了它们在活的 *choanoflagellates中的电活动。 NSERC资助的研究提供了第一个 * 证据，证明动物神经系统的电活动植根于 * 单细胞真核生物之间的细胞间钠离子信号传递提供的早期模板，这些单细胞真核生物可能起源于10亿年前的祖先。 自20世纪90年代以来，大卫·斯帕福德（Spafford）已经发表了关于电兴奋性的起源的文章 *（斯帕福德等人，1996 J. Exp. 199：941-948，1996），是少数几个谁是在 * 前沿的这一领域（如。Fux et*al. Frontiers in Physiology，2018）。 ** 心脏动作电位是用无脊椎动物心脏中独特的钠传导Cav 3 T型通道代替脊椎动物心脏中存在的Nav 1钠通道产生的（Senatore等人，J. Biol. Chem.，289（17）：11952-69，*2014; Guan等人，J. Gen Physiol.，在 * 审查2018年）。 这些不同的离子通道如何促进完整心脏中的电活动，例如心跳定时以及去极化和复极化波沿着连接的心脏细胞片的传播，仅使用MEA装置可获得。 ** 还需要MEA装置来分析具有钙传感器钙调蛋白的Cav 3型通道中钙传感的作用，发现钙传感器钙调蛋白调节心脏起搏（Chemin，J.，例如，292（49）：20010-20031，*2017）。 ** Yim博士专门研究神经元网络发育过程中纳米尺度变化的分析 *，并正在进行 * 使用MEA设备测量人类IPSC衍生神经元培养物中神经活性的研究 *。 ** 来自八个不同部门的十二名申请人正在进行涉及MEA设备的研究，用于 * 滑铁卢大学的共享多用户空间。

A multi-electrode array (MEA) recording system is a ground-breaking*device that enables researchers to monitor the electrical activity from*hundreds of cells at once, from single celled organisms to cells from tissues,*such as neurons or cardiomyocytes. An*MEA device is necessary to measure electrical activity in single cell*eukaryotes, which is not accessible in recordings with conventional*micro-electrodes. ******The Spafford lab has*cloned and expressed the first voltage–gated sodium and calcium channels from*single cell eukaryotes, and successfully measured their electrical activity in living*choanoflagellates using MEA demonstration units provided by the Axion*Biosystems Maestro Edge and Automate Scientific MED64 Presto in summer 2018. NSERC funded research provides the first*evidence that the electrical activity in animals' nervous systems, was rooted*on an early template provided from the inter-cellular sodium ion signalling between*single cell eukaryotes, in likely ancestors originating more than one billion*years ago. David Spafford has published*on the origins of electrical excitability since the 1990s (Spafford et al. 1996 J. Exp. Biol. 199: 941-948, 1996), and is one of a few who are at*the fore-front of this field (eg. Fux et*al. Frontiers in Physiology, 2018). ******The*cardiac action potential is generated with a uniquely invertebrate,*sodium-conducting Cav3 T-type channels in the invertebrate heart, in lieu of Nav1 sodium*channels present in the vertebrate heart (Senatore et al., J. Biol. Chem., 289(17):11952-69,*2014; Guan et al. J. Gen Physiol., in*review 2018). How these differing ion*channels contribute to the electrical activity in the intact heart such as heart*beat timing and the propagation of depolarizing and repolarizing waves along*the sheet of connected heart cells is only available using an MEA device. ******An MEA device is also required for analysing the*role of calcium-sensing in Cav3-Type channels with calcium-sensor calmodulin which*was discovered to regulate heart pace-making (Chemin, J., et al., J. Biol. Chem. 292(49):20010-20031,*2017). ******Dr. Yim specializes in analyses*of the nano-scale changes in developing neuronal networks, and has ongoing*research in measuring the nerve activity in human IPSC-derived neuronal cultures,*using an MEA device. ******Twelve plus*co-applicants from eight different departments have ongoing research involving an MEA device, for a shared multi-user space at the University of*Waterloo.