State-of-the-art multi-electrode array recording system for a core facility serving twelve plus faculty members from eight departments at the University of Waterloo

最先进的多电极阵列记录系统，用于为滑铁卢大学八个系的 12 多名教职人员提供服务的核心设施

• 批准号: RTI-2019-00069
• 负责人: Spafford, JDavid
• 金额: $ 8.6万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=665678
• 关键词: State; art; multi; electrode; array

A multi-electrode array (MEA) recording system is a ground-breaking*device that enables researchers to monitor the electrical activity from*hundreds of cells at once, from single celled organisms to cells from tissues,*such as neurons or cardiomyocytes. An*MEA device is necessary to measure electrical activity in single cell*eukaryotes, which is not accessible in recordings with conventional*micro-electrodes. ******The Spafford lab has*cloned and expressed the first voltage–gated sodium and calcium channels from*single cell eukaryotes, and successfully measured their electrical activity in living*choanoflagellates using MEA demonstration units provided by the Axion*Biosystems Maestro Edge and Automate Scientific MED64 Presto in summer 2018. NSERC funded research provides the first*evidence that the electrical activity in animals' nervous systems, was rooted*on an early template provided from the inter-cellular sodium ion signalling between*single cell eukaryotes, in likely ancestors originating more than one billion*years ago. David Spafford has published*on the origins of electrical excitability since the 1990s (Spafford et al. 1996 J. Exp. Biol. 199: 941-948, 1996), and is one of a few who are at*the fore-front of this field (eg. Fux et*al. Frontiers in Physiology, 2018). ******The*cardiac action potential is generated with a uniquely invertebrate,*sodium-conducting Cav3 T-type channels in the invertebrate heart, in lieu of Nav1 sodium*channels present in the vertebrate heart (Senatore et al., J. Biol. Chem., 289(17):11952-69,*2014; Guan et al. J. Gen Physiol., in*review 2018). How these differing ion*channels contribute to the electrical activity in the intact heart such as heart*beat timing and the propagation of depolarizing and repolarizing waves along*the sheet of connected heart cells is only available using an MEA device. ******An MEA device is also required for analysing the*role of calcium-sensing in Cav3-Type channels with calcium-sensor calmodulin which*was discovered to regulate heart pace-making (Chemin, J., et al., J. Biol. Chem. 292(49):20010-20031,*2017). ******Dr. Yim specializes in analyses*of the nano-scale changes in developing neuronal networks, and has ongoing*research in measuring the nerve activity in human IPSC-derived neuronal cultures,*using an MEA device. ******Twelve plus*co-applicants from eight different departments have ongoing research involving an MEA device, for a shared multi-user space at the University of*Waterloo.

多电极阵列（MEA）记录系统是一种突破性的设备，使研究人员能够同时监测数百个细胞的电活动，从单细胞生物到组织细胞，如神经元或心肌细胞。MEA设备是测量单细胞真核生物电活动所必需的，这在传统的微电极记录中是无法实现的。****** Spafford实验室克隆并表达了来自单细胞真核生物的第一个电压门控钠和钙通道，并在2018年夏季使用由Axion Biosystems Maestro Edge和automation Scientific MED64 Presto提供的MEA演示单元成功测量了它们在活体choanoflagellate中的电活动。NSERC资助的研究提供了第一个证据，证明动物神经系统的电活动根植于单细胞真核生物之间的细胞间钠离子信号传递提供的早期模板，其祖先可能起源于10亿多年前。David Spafford自20世纪90年代以来发表了关于电兴奋性起源的文章（Spafford et al. 1996 J. Exp. Biol. 199: 941-948, 1996），并且是少数处于该领域前沿的人之一。Fux et al。生理学前沿，2018)。******心脏动作电位是由无脊椎动物心脏中独特的钠传导Cav3 t型通道产生的，而不是脊椎动物心脏中存在的Nav1钠通道（Senatore et al.， J. Biol.）。化学。, 289 (17): 11952 - 69 * 2014;Guan等。[j] Gen physil。（2018年*回顾）。这些不同的离子通道如何影响完整心脏中的电活动，如心跳定时和沿连接的心脏细胞片的去极化和复极化波的传播，只有使用MEA设备才能获得。******还需要一个MEA设备来分析钙传感在cav3型通道中的作用，钙传感钙调蛋白*被发现可以调节心脏起搏（Chemin， J., et al.， J. Biol.）。292化学(49):20010 - 20031 * 2017)。* * * * * *博士。Yim擅长分析发育中的神经元网络的纳米级变化，并正在进行使用MEA设备测量人类ipsc衍生的神经元培养物中的神经活动的研究。******来自8个不同院系的12名以上共同申请者正在进行一项涉及MEA设备的研究，该设备将用于滑铁卢大学的共享多用户空间。