Investigating the molecular mechanisms of plant growth promotion in canola, by Delftia acidovorans

Lallemand制造商BioBoost，一种土壤活性的促进生长的根际细菌(Delftia Acidovorans)，作为紧急喷雾应用于油菜。在2007至2012年的农业田间试验中，应用BioBoost平均每英亩增产2蒲式耳。该项目是Lallemand和萨斯喀彻温省里贾纳大学的研究人员合作的结果，目的是调查BioBoost接种剂促进植物生长的遗传基础。该项目涉及使用高通量DNA测序来对Delftia acidovorans菌株的基因组进行排序。组装的基因组将形成后续研究的基础，以了解嗜酸双胞菌在油菜根际的定植机制和促进植物生长的作用。使用高通量突变方法(INSeq)进行全基因组筛选，我们将识别适应根际环境的基因。同时，RNA-seq图谱将被用来研究嗜酸葡萄球菌在根际定殖时的转录组。这两种方法结合起来，将为了解根际定殖机制和可能与植物生长促进相关的产酸假单胞菌的代谢活动提供高分辨率的见解。该项目的第二阶段将涉及使用INSeq和定向突变来研究接种菌中的硫氧化基因网络，并测量作为植物生长促进因子的硫氧化的程度。

Lallemand manufacturers BioBoost, a soil-active growth-promoting rhizobacteria (Delftia acidovorans) that is applied to canola as a post-emergent spray. In agricultural field trials between 2007 and 2012 BioBoost application resulted in an average yield increase of two bushels per acre. This project is the result of a collaboration between Lallemand and researchers at the University of Regina, Saskatchewan to investigate the genetic basis of the plant growth promotive effects of the BioBoost inoculant. The project involves the use of high throughput DNA sequencing to sequence the genome of the Delftia acidovorans strain. The assembled genome will form the basis of subsequent investigations on the mechanisms of D. acidovorans colonization of the canola rhizosphere and plant growth promotive effects. Using a high throughput mutagenesis approach (INSeq) to undertake a genome wide screen we will identify genes required for fitness in colonizing the rhizosphere environment. Simultaneously RNA-seq profiling will be used to study the transcriptome of D. acidovorans as it colonizes the rhizosphere. Together, these two methodologies will provide high resolution insights into the mechanisms of rhizosphere colonization and the metabolic activities of D. acidovorans that may be correlated to plant growth promotion. The second phase of the project will involve using INSeq and directed mutagenesis to study sulphur oxidation gene networks in the D. acidovorans inoculant strain and measure the magnitude of sulphur oxidation as a plant growth promotive factor.