In vitro and in vivo evaluation of equine probiotics

马益生菌的体外和体内评价

• 批准号: 533783-2018
• 负责人: Pearson, Wendy
• 金额: $ 1.73万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=679498
• 关键词: vitro; vivo; evaluation; equine; probiotics

The equine nutraceuticals industry in Canada is the largest and most rapidly growing division of the domestic veterinary supplements sector. The Goal of the current study is to evaluate the in vitro and in vivo impact of a Canadian dietary probiotic ('ProbioPLUS'; Selected Bioproducts Inc; Guelph ON) (PBP) on the equine hind-gut microflora during an antibiotic challenge. The objectives of the current study are: 1) Create an in vitro model of the equine cecum using an anaerobic culture chamber; 2) use the model created in Objective 1 to determine effects of a common equine antibiotic (Sulfadiazine/Trimethoprim; STP) on pH and bacterial metabolite (acetate, propionate and butyrate) production in the presence and absence of a simulated digest of PBP; and 3) evaluate effect of oral antibiotics on fecal microbial profile of horses receiving a diet containing PBP (0 or 66g/day) for 10 days. Objectives 1 and 2 will develop an in vitro simulation of the equine cecal microbial environment, and this model will be used to evaluate the impact of PPB on microbial disruption by antibiotics. Post-mortem cecal fluid from horses will be maintained in a continuous flow anaerobic chamber, and pH, total volatile fatty acids, acetate, propionate, butyrate, lactate and RNA sequencing of microbial populations will be measured. Once established, we will then introduce a common equine antibiotic (Sulfadiazine/Trimethoprim; STP) in the presence or absence of PBP, and determine its effect on the outcome measures described above. Objective 3 will evaluate the effect of oral STP on fecal quality and microbial profile in horses receiving PBP (0 or 66g/day) for 10 days prior to, during, and days following 10 days of STP administration. Antibiotic use in horses is often associated with potentially life-threatening colitis in horses, likely due to a disruption of the gastrointestinal microbiome. This study will provide important new information on the effect of dietary probiotics on the hind-gut microbiota in antibiotic-treated horses, and will determine the value of co-administration of antibiotics and probiotics in horses as well as other veterinary species and humans.

加拿大的马营养食品行业是国内兽药补充剂行业中规模最大、增长最快的部门。本研究的目的是评估加拿大饮食益生菌(ProBioPLUS；Selected BioProducts Inc；Guelph On)(PBP)(PBP)在抗生素挑战期间对马后肠微生物区系的体外和体内影响。本研究的目标是：1)使用厌氧培养箱建立马盲肠的体外模型；2)使用目标1中创建的模型来确定在存在和不存在PBP模拟消化的情况下，常见的马用抗生素(磺胺嘧啶/甲氧苄啶；STP)对pH值和细菌代谢物(醋酸盐、丙酸盐和丁酸盐)产生的影响；以及3)评估口服抗生素对接受含有PBP(0或66g/天)的饲料10天的马的粪便微生物谱的影响。目标1和目标2将建立马盲肠微生物环境的体外模拟，该模型将用于评估PPB对抗生素破坏微生物的影响。马死后的盲肠液将被保存在连续流动的厌氧室中，并将测量微生物种群的pH值、总挥发性脂肪酸、醋酸盐、丙酸、丁酸盐、乳酸和RNA测序。一旦确定，我们将在存在或不存在PBP的情况下引入一种常见的马类抗生素(磺胺嘧啶/甲氧苄啶；STP)，并确定其对上述结果衡量标准的影响。目的3评价口服STP对服用PBP(0或66g/d)的马在给药前、中、后10天粪便质量和微生物谱的影响。马使用抗生素经常与潜在威胁生命的马结肠炎有关，这可能是由于胃肠道微生物群的破坏。这项研究将为饲料中益生菌对抗生素处理过的马的后肠微生物区系的影响提供重要的新信息，并将确定在马以及其他兽医物种和人类中联合使用抗生素和益生菌的价值。