NANO-FORMULATION OF ANTIMICROBIAL PEPTIDES FOR THE MANAGEMENT OF BACTERIAL INFECTIONS

近年来，深入研究的重点是寻找抗微生物疗法。抗菌肽(AMPs) -由12-45个氨基酸组成的阳离子肽，已成为传统抗生素的有希望的替代品。抗菌肽可以阻止细菌的增殖，因此细菌不会产生任何抗药性。虽然抗菌肽被认为是一种新的抗生素，也是一种很好的替代药物，但在临床应用之前还需要解决几个问题。amp在到达感染部位之前就容易被人体内存在的蛋白水解酶降解。因此，有必要设计amp的递送载体，以保护它们免受早期降解，并确保它们直接递送到受感染的部位。通过对聚合物纳米颗粒/水凝胶技术的广泛研究，我们建议使用聚合物纳米颗粒/纳米凝胶/水凝胶作为amp的纳米载体。聚合物可以以多种方式进行工程设计，使其无毒，可以很容易地包裹分子或大分子，并且可以在人体内代谢。我们将进一步改进负载amp的聚合物纳米颗粒，使其具有特定的管理途径，从而提高其临床疗效。为了用于呼吸输送，聚合物纳米颗粒将被涂上天然黏合剂聚合物，并通过雾化输送。对于感染性伤口的治疗，它们将被包裹在纤维素纳米晶体水凝胶中，并用作伤口敷料。我们假设负载amp的聚合物纳米颗粒可以成功克服抗生素耐药菌株引起的感染。在诊所中使用负载amp的纳米颗粒可以潜在地减少我们对日常使用的传统抗生素的依赖，并防止细菌耐药性的发展。它将改善患者的整体健康状况，并降低治疗成本。该项目将为了解抗菌肽与聚合物纳米颗粒/水凝胶的相互作用、肽包封和释放动力学的功效以及它们的生物学特性提供一个框架。

In recent years, intensive research has focused on finding antimicrobial therapies. Antimicrobial peptides (AMPs) - cationic peptides consisting of 12-45 amino acids, have emerged as promising alternatives to conventional antibiotics. AMPs can stop bacteria from proliferation and thus bacteria could not develop any resistance to them. Though AMPs are regarded as a new class of antibiotics and also a good alternative, several problems need to be addressed before their use in the clinical field. The AMPs are easily prone to degradation by proteolytic enzymes present in human body even before reaching the infected site. As a result, there is a need to engineer delivery carriers for AMPs to protect them from early degradation and ensuring their direct delivery to the infected sites. With our extensive research on polymer nanoparticles/hydrogels technology, we suggest the use polymer nanoparticles/nanogels/hydrogels as AMPs nanocarriers. Polymers can be engineered in a multitude of ways such that they are non-toxic, can easily encapsulate molecules or macromolecules and can be metabolized in human body. The AMPs-loaded polymer nanoparticles will be further modified for specific management routes which will improve their clinical efficacy. In order to be used for respiratory delivery, polymer nanoparticles will be coated with natural muco-adhesive polymers and delivered via nebulisation. For the treatment of infectious wounds, they will be entrapped in cellulose nanocrystal based hydrogels and used as wound dressings. We hypothesize that polymer nanoparticles loaded with AMPs can successfully overcome infections caused by antibiotic-resistant bacterial strains. The use of AMPs-loaded nanoparticles in clinics can potentially reduce our reliance on conventional antibiotics that are used on a daily basis and prevent progress of bacterial resistance. It will improve the overall health of patients and also decrease treatment cost. This project will provide a framework in understanding the interactions of selected antimicrobial peptides and polymer nanoparticles/hydrogels, efficacy of peptide encapsulation and release kinetics, and their biological properties.