High activity enzymatic cocktail for efficient degradation of petroleum hydrocarbons

用于高效降解石油烃的高活性酶混合物

• 批准号: 536510-2018
• 负责人: Brar, Satinder
• 金额: $ 5.1万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=691531
• 关键词: activity; enzymatic; cocktail; efficient; degradation

Spilling and release of petroleum hydrocarbons to the environment caused serious contamination to the soil and groundwater. These compounds deteriorate the quality of soil for agriculture and can be toxic to different organisms. The proposed work will optimize one of the current biological methods for addressing the problem of soil contamination. A. borkumensis is a bacterium able to assimilate different hydrocarbons by producing a set of enzymes such as esterase, lipase and alkane hydroxylase. In lab experiments, A. borkumensis showed high degradation efficiency when it was used as live culture or enzyme extracts. However, practical limitations (such as low temperature and oxygen), as well as economical concerns, challenge the application of A. borkumensis in these two forms. The objective of this proposal is to optimize the production of enzymes in big scales and therefore eliminating the need for the concentration of enzyme. This approach will be used to integrate process optimization with industrial-scale production of enzymes. Our pathway to reach this goal starts from optimizing the enzyme production at small scale by modifying the media composition (addition of inducers) and ends into scaled-up fermenter (2,000 L) working at semi-continuous mode with in-situ sonication Finally, the cost evaluation of the whole process will facilitate the choice of the best production scenario for optimal application.The Highly Qualified Personnel (HQP), will acquire essential skills for future careers in Canada. They will learn techniques for optimization and production of enzymes at large scale and their application in field conditions. Specifically, they will be trained in biotechnology, microbiology, chemistry, and biochemical engineering to obtain enzyme formulations that meet the technical and economical requirements.

石油碳氢化合物向环境中的溢出和释放对土壤和地下水造成了严重污染。这些化合物使农业土壤的质量恶化，并对不同的生物体有毒。拟议的工作将优化目前用于解决土壤污染问题的生物方法之一。A. Borkenensis是一种能够通过产生一组酶如酯酶、脂肪酶和烷烃羟化酶来同化不同烃的细菌。在实验室实验中，A.当用作活菌培养物或酶提取物时，Borkenensis显示出高的降解效率。然而，实际的限制（如低温和氧气），以及经济上的考虑，挑战A。在这两种形式中的博伊尔人。该提案的目的是优化大规模酶的生产，从而消除对酶浓缩的需要。该方法将用于将工艺优化与酶的工业规模生产相结合。我们实现这一目标的途径是通过改变培养基组成来优化小规模的酶生产最后，对整个工艺的成本评估将有助于选择最佳的生产方案，以实现最佳的应用。高素质的人员（HQP），将获得在加拿大未来职业生涯的基本技能。他们将学习大规模优化和生产酶的技术及其在田间条件下的应用。具体而言，他们将接受生物技术、微生物学、化学和生物化学工程方面的培训，以获得符合技术和经济要求的酶制剂。