High activity enzymatic cocktail for efficient degradation of petroleum hydrocarbons
用于高效降解石油烃的高活性酶混合物
基本信息
- 批准号:536510-2018
- 负责人:
- 金额:$ 5.1万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Collaborative Research and Development Grants
- 财政年份:2019
- 资助国家:加拿大
- 起止时间:2019-01-01 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Spilling and release of petroleum hydrocarbons to the environment caused serious contamination to the soil and groundwater. These compounds deteriorate the quality of soil for agriculture and can be toxic to different organisms. The proposed work will optimize one of the current biological methods for addressing the problem of soil contamination. A. borkumensis is a bacterium able to assimilate different hydrocarbons by producing a set of enzymes such as esterase, lipase and alkane hydroxylase. In lab experiments, A. borkumensis showed high degradation efficiency when it was used as live culture or enzyme extracts. However, practical limitations (such as low temperature and oxygen), as well as economical concerns, challenge the application of A. borkumensis in these two forms. The objective of this proposal is to optimize the production of enzymes in big scales and therefore eliminating the need for the concentration of enzyme. This approach will be used to integrate process optimization with industrial-scale production of enzymes. Our pathway to reach this goal starts from optimizing the enzyme production at small scale by modifying the media composition (addition of inducers) and ends into scaled-up fermenter (2,000 L) working at semi-continuous mode with in-situ sonication Finally, the cost evaluation of the whole process will facilitate the choice of the best production scenario for optimal application.The Highly Qualified Personnel (HQP), will acquire essential skills for future careers in Canada. They will learn techniques for optimization and production of enzymes at large scale and their application in field conditions. Specifically, they will be trained in biotechnology, microbiology, chemistry, and biochemical engineering to obtain enzyme formulations that meet the technical and economical requirements.
石油碳氢化合物向环境中的溢出和释放对土壤和地下水造成了严重污染。这些化合物使农业土壤的质量恶化,并对不同的生物体有毒。拟议的工作将优化目前用于解决土壤污染问题的生物方法之一。A. Borkenensis是一种能够通过产生一组酶如酯酶、脂肪酶和烷烃羟化酶来同化不同烃的细菌。在实验室实验中,A.当用作活菌培养物或酶提取物时,Borkenensis显示出高的降解效率。然而,实际的限制(如低温和氧气),以及经济上的考虑,挑战A。在这两种形式中的博伊尔人。该提案的目的是优化大规模酶的生产,从而消除对酶浓缩的需要。该方法将用于将工艺优化与酶的工业规模生产相结合。我们实现这一目标的途径是通过改变培养基组成来优化小规模的酶生产最后,对整个工艺的成本评估将有助于选择最佳的生产方案,以实现最佳的应用。高素质的人员(HQP),将获得在加拿大未来职业生涯的基本技能。他们将学习大规模优化和生产酶的技术及其在田间条件下的应用。具体而言,他们将接受生物技术、微生物学、化学和生物化学工程方面的培训,以获得符合技术和经济要求的酶制剂。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Brar, Satinder其他文献
Brar, Satinder的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Brar, Satinder', 18)}}的其他基金
Fate and transport of evolving antibiotics and their removal using novel bio-oxidation technologies
不断进化的抗生素的命运和运输以及使用新型生物氧化技术去除它们
- 批准号:
RGPIN-2020-06067 - 财政年份:2022
- 资助金额:
$ 5.1万 - 项目类别:
Discovery Grants Program - Individual
Fate and transport of evolving antibiotics and their removal using novel bio-oxidation technologies
不断进化的抗生素的命运和运输以及使用新型生物氧化技术去除它们
- 批准号:
RGPIN-2020-06067 - 财政年份:2021
- 资助金额:
$ 5.1万 - 项目类别:
Discovery Grants Program - Individual
Fate and transport of evolving antibiotics and their removal using novel bio-oxidation technologies
不断进化的抗生素的命运和运输以及使用新型生物氧化技术去除它们
- 批准号:
RGPIN-2020-06067 - 财政年份:2020
- 资助金额:
$ 5.1万 - 项目类别:
Discovery Grants Program - Individual
Automated Parallel Bioreactor Systems for High Throughput Optimization of Bioengineered Processes
用于生物工程过程高通量优化的自动化并行生物反应器系统
- 批准号:
RTI-2021-00463 - 财政年份:2020
- 资助金额:
$ 5.1万 - 项目类别:
Research Tools and Instruments
Novel approach to production of high-value biochemicals and simultaneous removal of emerging contaminants using waste biomass by biofilm reactors
通过生物膜反应器利用废弃生物质生产高价值生化产品并同时去除新兴污染物的新方法
- 批准号:
355254-2013 - 财政年份:2019
- 资助金额:
$ 5.1万 - 项目类别:
Discovery Grants Program - Individual
Enhanced biogas production and high-end valorization of digestate from anaerobic digestion of organic residuals
提高沼气产量并实现有机残渣厌氧消化消化物的高端增值
- 批准号:
531209-2018 - 财政年份:2019
- 资助金额:
$ 5.1万 - 项目类别:
Collaborative Research and Development Grants
Novel Bacterial Blend to Enhance Biomethanation of Municipal Sewage Sludge
新型细菌混合物可增强城市污水污泥的生物甲烷化
- 批准号:
539944-2019 - 财政年份:2019
- 资助金额:
$ 5.1万 - 项目类别:
Engage Grants Program
Advanced Molecular Sequencing For Qualitative And Quantitative Environmental Analyses
用于定性和定量环境分析的先进分子测序
- 批准号:
RTI-2020-00758 - 财政年份:2019
- 资助金额:
$ 5.1万 - 项目类别:
Research Tools and Instruments
相似海外基金
Integrated Computational and Mechanistic Investigation on New Reactivity and Selectivity in Emerging Enzymatic Reactions
新兴酶反应中新反应性和选择性的综合计算和机理研究
- 批准号:
2400087 - 财政年份:2024
- 资助金额:
$ 5.1万 - 项目类别:
Standard Grant
Development of programmable nanomachines towards the enzymatic synthesis of peptide oligonucleotide conjugates
开发用于肽寡核苷酸缀合物酶促合成的可编程纳米机器
- 批准号:
EP/X019624/1 - 财政年份:2024
- 资助金额:
$ 5.1万 - 项目类别:
Fellowship
Haemotoxic and cytotoxic snake venom metalloproteinases - production, enzymatic specificity, snakebite treatment, and biomedical use
血液毒性和细胞毒性蛇毒金属蛋白酶 - 生产、酶特异性、蛇咬伤治疗和生物医学用途
- 批准号:
BB/Y007581/1 - 财政年份:2024
- 资助金额:
$ 5.1万 - 项目类别:
Research Grant
Enhancing cellulase activity through single-molecule imaging and protein engineering as a testbed for understanding and improving enzymatic deconstruction of insoluble substrates
通过单分子成像和蛋白质工程增强纤维素酶活性,作为理解和改进不溶性底物酶解构的测试平台
- 批准号:
2301377 - 财政年份:2023
- 资助金额:
$ 5.1万 - 项目类别:
Standard Grant
SBIR Phase I: Development of an enzymatic method to produce compounds found in human milk at commercial scale
SBIR 第一阶段:开发酶法以商业规模生产母乳中的化合物
- 批准号:
2304250 - 财政年份:2023
- 资助金额:
$ 5.1万 - 项目类别:
Standard Grant
Exploration for thermophilic nicotinamidephosphorybosyl transferases and their application to enzymatic production of NMN
嗜热烟酰胺磷酸核糖基转移酶的探索及其在酶法生产 NMN 中的应用
- 批准号:
23H02128 - 财政年份:2023
- 资助金额:
$ 5.1万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Accessing and Expanding Natural Products Chemical Diversity by Big-data Analysis and Biosynthetic Investigation
通过大数据分析和生物合成研究获取和扩大天然产物化学多样性
- 批准号:
10714466 - 财政年份:2023
- 资助金额:
$ 5.1万 - 项目类别:
Regulation of erythroid iron metabolism by the CLPX unfoldase
CLPX 解折叠酶对红细胞铁代谢的调节
- 批准号:
10716494 - 财政年份:2023
- 资助金额:
$ 5.1万 - 项目类别:
Elucidating the dynamical and structural molecular factors at the origin of non-enzymatic protein-protein and protein-DNA cross-links
阐明非酶蛋白质-蛋白质和蛋白质-DNA 交联起源的动力学和结构分子因素
- 批准号:
10709399 - 财政年份:2023
- 资助金额:
$ 5.1万 - 项目类别: