High activity enzymatic cocktail for efficient degradation of petroleum hydrocarbons

用于高效降解石油烃的高活性酶混合物

• 批准号: 536510-2018
• 负责人: Brar, Satinder
• 金额: $ 5.1万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=691531
• 关键词: activity; enzymatic; cocktail; efficient; degradation

Spilling and release of petroleum hydrocarbons to the environment caused serious contamination to the soil and groundwater. These compounds deteriorate the quality of soil for agriculture and can be toxic to different organisms. The proposed work will optimize one of the current biological methods for addressing the problem of soil contamination. A. borkumensis is a bacterium able to assimilate different hydrocarbons by producing a set of enzymes such as esterase, lipase and alkane hydroxylase. In lab experiments, A. borkumensis showed high degradation efficiency when it was used as live culture or enzyme extracts. However, practical limitations (such as low temperature and oxygen), as well as economical concerns, challenge the application of A. borkumensis in these two forms. The objective of this proposal is to optimize the production of enzymes in big scales and therefore eliminating the need for the concentration of enzyme. This approach will be used to integrate process optimization with industrial-scale production of enzymes. Our pathway to reach this goal starts from optimizing the enzyme production at small scale by modifying the media composition (addition of inducers) and ends into scaled-up fermenter (2,000 L) working at semi-continuous mode with in-situ sonication Finally, the cost evaluation of the whole process will facilitate the choice of the best production scenario for optimal application.The Highly Qualified Personnel (HQP), will acquire essential skills for future careers in Canada. They will learn techniques for optimization and production of enzymes at large scale and their application in field conditions. Specifically, they will be trained in biotechnology, microbiology, chemistry, and biochemical engineering to obtain enzyme formulations that meet the technical and economical requirements.

石油碳氢化合物泄漏和释放到环境中对土壤和地下水造成严重污染。这些化合物会恶化农业土壤的质量，并且可能对不同的生物体有毒。拟议的工作将优化当前解决土壤污染问题的生物方法之一。 A. borkumensis 是一种能够通过产生酯酶、脂肪酶和烷烃羟化酶等一系列酶来同化不同碳氢化合物的细菌。在实验室实验中，A. borkumensis 当用作活培养物或酶提取物时表现出较高的降解效率。然而，实际限制（例如低温和氧气）以及经济问题对博尔库曼西斯在这两种形式中的应用提出了挑战。该提案的目的是优化大规模酶的生产，从而消除对酶浓缩的需求。该方法将用于将工艺优化与酶的工业规模生产相结合。我们实现这一目标的途径从通过修改培养基成分（添加诱导剂）优化小规模酶生产开始，到以半连续模式原位超声处理的放大发酵罐（2,000 L）结束，最后，整个过程的成本评估将有助于选择最佳生产方案以实现最佳应用。高素质人员（HQP）将获得在加拿大未来职业生涯的基本技能。他们将学习大规模优化和生产酶的技术及其在现场条件下的应用。具体来说，他们将接受生物技术、微生物学、化学和生化工程方面的培训，以获得满足技术和经济要求的酶制剂。