Custom platform for evaluation of conserved mechanisms of fever

用于评估发热保守机制的定制平台

• 批准号: RTI-2020-00353
• 负责人: Barreda, Daniel
• 金额: $ 6.55万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=692389
• 关键词: Custom; platform; evaluation; conserved; mechanisms

My lab has recently identified the most conclusive functional arguments for the conservation of fever for over 500 million years of evolution. The results have opened a number of meaningful opportunities for pan-Canadian and international collaborative research. Unfortunately, experiments take too long (778 days to simply conduct the fever inducing and monitoring components of experiments in first publication figure; nearly 5 years to complete first publication). We absolutely require upgraded equipment to increase our 1) Capacity to scale--up evaluations to larger cohorts, and 2) Process longer fever evaluation datasets. This will greatly improve throughput and allow us to tackle central questions for this research. For example, it will be critical to assess the contributions of fever to reduction of disease transmission since these require mixed populations (i.e. animals infected at different levels) in a cohabitation format. This is important to understand key public and animal health implications. A plan is already in place to ensure sustainability of this equipment, including a partnership to continue to add functionality to the custom analytical software suite. There is no conceptual or budgetary overlap between the proposed infrastructure and requests under any other current or ongoing grant application. In all, the requested analysis system will help position Canada at the forefront of fever research, and provide a viable avenue to translate fundamental comparative biological knowledge into applications that benefit Canadians. It will also open new opportunities for collaborations in Canada and abroad. 24 highly qualified personnel (HQP) will directly apply the requested infrastructure to their training over the next 3 years (2 PDF, 2 PhD, 3 MSc, 14 UGs, 2 Techs). Five of these trainees are already in place. My lab has a proven record of excellence in HQP development (74 trainees; 109 awards including 2 prestigious NSERC Vanier Scholarships). The proposed technology suite will provide a new dimension in this interdisciplinary training and opportunities for career niche development. As our research advances into applications locally and internationally there will be a host of opportunities for HQP that will be highly valued and marketable.

我的实验室最近发现了在超过5亿年的进化过程中，关于发烧守恒的最确凿的功能论据。这一结果为泛加拿大和国际合作研究开辟了许多有意义的机会。遗憾的是，实验花费的时间太长(778天来简单地进行实验的发热和监测部分，在第一次发表的图中；近5年才完成第一次发表)。我们绝对需要升级设备来提高我们的能力，1)将评估扩大到更大的队列，以及2)处理更长的发烧评估数据集。这将极大地提高吞吐量，并使我们能够解决这项研究的中心问题。例如，评估发烧对减少疾病传播的贡献将是至关重要的，因为这需要以同居形式混合种群(即感染不同水平的动物)。这对于理解关键的公共和动物健康影响很重要。已经制定了一项计划，以确保这种设备的可持续性，包括继续为定制分析软件套件增加功能的合作伙伴关系。拟议的基础设施与任何其他当前或正在进行的赠款申请下的请求之间在概念上或预算上没有重叠。总之，所要求的分析系统将有助于将加拿大定位在发烧研究的前沿，并提供一个可行的途径，将基本的比较生物学知识转化为造福加拿大人的应用。它还将为加拿大和海外的合作打开新的机会。24名高素质人员(HQP)将在未来3年将所需的基础设施直接应用于他们的培训(2名PDF、2名博士、3名硕士、14名UGS、2名理工科)。这些受训人员中有五人已经就位。我的实验室在HQP开发方面拥有卓越的记录(74名学员；109个奖项，其中包括2个著名的NSERC Vanier奖学金)。拟议的技术套件将为这一跨学科培训提供新的层面，并为职业发展提供机会。随着我们的研究在本地和国际上应用，HQP将会有许多非常有价值和有市场的机会。