High throughput flow cytometer for the Michael Smith Laboratories

新一代高通量流式细胞仪的申请来自四个nserc资助的申请人，以及位于不列颠哥伦比亚大学（UBC）跨学科的Michael Smith实验室（MSL）的另外四个用户。所要求的仪器，与自动进样器相连接的Invitrogen tune NxT，将使我们的团队能够为自然科学项目执行和开发广泛的一系列检测，其中基于表面标记和/或荧光蛋白对大颗粒和不同类型的细胞（细菌，酵母，植物和哺乳动物）进行定量。目前，在MSL中没有高通量或足够快速和敏感的设备来支持拟议的应用。相反，不同的团队成员要么使用过时的仪器，不能再更新，要么使用位于UBC流动设施的仪器，这排除了许多实际原因提出的应用。因此，所要求的仪器将是关键，既要维持目前的计划，又要促进一群在各自领域表现出色的研究人员开发创新方法。在过去五年中，申请人和主要相关用户总共出版了250多本出版物。所要求的仪器将是训练生物科学方面的高素质人员的中心。MSL重视公平、多元化和包容，并为学员提供了一个在性别、种族背景和教育方面都非常多样化的环境。MSL有300多名学员，其中申请实验室有48人，主要用户实验室有82人。我们预计，所要求的流式细胞仪将在未来五年内参加75-125名研究人员的培训，这将有利于生物技术部门。***********

This application for a next generation high-throughput flow cytometer is from a cluster of four NSERC-funded applicants together with four additional users located in the interdisciplinary Michael Smith Laboratories (MSL) at the University of British Columbia (UBC). The requested instrument, the Invitrogen Attune NxT coupled to an auto-sampler, will enable our team to perform and develop a broad series of assays for projects in natural science, in which large particles and different types of cells (bacteria, yeast, plant and mammalian) are quantified based on surface markers and/or fluorescent proteins. Currently, there is no high-throughput or sufficiently fast and sensitive equipment within the MSL that supports the proposed applications. Instead, the different team members have worked either with an outdated instrument that can no longer be updated or with instruments distantly located in the UBC flow facility, which precludes many of the proposed applications for practical reasons. Therefore, the requested instrument will be key, both to sustain the current programs and to facilitate the development of innovative approaches by a cluster of researchers who have clearly demonstrated excellence in their respective fields. Collectively, the applicants and main associated users have generated over 250 publications in the past 5 years. The requested instrument will be central for the training of highly qualified personnel (HQP) in biological sciences. MSL values equity, diversity and inclusion, and offers an environment with a great diversity of trainees both in term of gender, ethnical background and education. There are over 300 trainees in the MSL, including 48 in the applicants' and 82 in the main users' laboratories. We anticipate that the requested flow cytometer will participate to the training of a large pool of 75-125 researchers in the next five years that will be beneficial for the biotechnology sector. ***********