High throughput flow cytometer for the Michael Smith Laboratories

下一代高通量流式细胞仪的申请来自不列颠哥伦比亚大学(UBC)跨学科迈克尔·史密斯实验室(MSL)的四名NSERC资助的申请者和另外四名用户。所要求的仪器Invitgen Attune NXT与自动取样器相结合，将使我们的团队能够为自然科学项目执行和开发一系列广泛的分析方法，其中大颗粒和不同类型的细胞(细菌、酵母、植物和哺乳动物)根据表面标记和/或荧光蛋白进行量化。目前，MSL内没有高吞吐量或足够快和灵敏的设备来支持拟议的应用。取而代之的是，不同的团队成员要么使用不能再更新的过时仪器，要么使用位于UBC Flow设施中的仪器，由于实际原因，这排除了许多拟议的应用。因此，所要求的文书将是维持当前方案和促进一群研究人员开发创新方法的关键，这些研究人员显然在各自的领域表现出色。在过去5年中，申请者和主要相关用户总共出版了250多份出版物。所要求的仪器将是培训高素质生物科学人员(HQP)的核心。MSL重视公平、多样性和包容性，并提供了一个培训人员在性别、种族背景和教育方面都非常多样化的环境。MSL有300多名学员，其中48人在申请人的实验室，82人在主要用户的实验室。我们预计，所要求的流式细胞仪将在未来五年内参与培训75-125名研究人员，这将有利于生物技术部门。***********

This application for a next generation high-throughput flow cytometer is from a cluster of four NSERC-funded applicants together with four additional users located in the interdisciplinary Michael Smith Laboratories (MSL) at the University of British Columbia (UBC). The requested instrument, the Invitrogen Attune NxT coupled to an auto-sampler, will enable our team to perform and develop a broad series of assays for projects in natural science, in which large particles and different types of cells (bacteria, yeast, plant and mammalian) are quantified based on surface markers and/or fluorescent proteins. Currently, there is no high-throughput or sufficiently fast and sensitive equipment within the MSL that supports the proposed applications. Instead, the different team members have worked either with an outdated instrument that can no longer be updated or with instruments distantly located in the UBC flow facility, which precludes many of the proposed applications for practical reasons. Therefore, the requested instrument will be key, both to sustain the current programs and to facilitate the development of innovative approaches by a cluster of researchers who have clearly demonstrated excellence in their respective fields. Collectively, the applicants and main associated users have generated over 250 publications in the past 5 years. The requested instrument will be central for the training of highly qualified personnel (HQP) in biological sciences. MSL values equity, diversity and inclusion, and offers an environment with a great diversity of trainees both in term of gender, ethnical background and education. There are over 300 trainees in the MSL, including 48 in the applicants' and 82 in the main users' laboratories. We anticipate that the requested flow cytometer will participate to the training of a large pool of 75-125 researchers in the next five years that will be beneficial for the biotechnology sector. ***********