High throughput flow cytometer for the Michael Smith Laboratories

下一代高通量流式细胞仪的这一申请来自四名NSERC资助的申请人以及位于不列颠哥伦比亚省大学（UBC）跨学科Michael Smith实验室（MSL）的四名其他用户。所要求的仪器，英杰Attune NxT耦合到自动进样器，将使我们的团队能够执行和开发一系列广泛的测定项目在自然科学中，其中大颗粒和不同类型的细胞（细菌，酵母，植物和哺乳动物）是基于表面标记和/或荧光蛋白定量。目前，在MSL中没有支持拟议应用的高通量或足够快和灵敏的设备。相反，不同的团队成员要么使用无法更新的过时仪器，要么使用位于UBC流量设施中的远程仪器，这排除了许多出于实际原因而提出的应用。因此，所要求的工具将是关键，既要维持目前的方案，又要促进在各自领域表现出卓越成就的一批研究人员开发创新方法。申请人和主要相关用户在过去5年中共发表了250多篇出版物。所要求的仪器将是生物科学高素质人员培训的核心。MSL重视公平，多样性和包容性，并提供了一个在性别，种族背景和教育方面都具有极大多样性的学员环境。MSL有300多名受训人员，其中48人在申请者实验室，82人在主要用户实验室。我们预计，所要求的流式细胞仪将在未来五年内参加对75-125名研究人员的培训，这将有利于生物技术部门。 ***********

This application for a next generation high-throughput flow cytometer is from a cluster of four NSERC-funded applicants together with four additional users located in the interdisciplinary Michael Smith Laboratories (MSL) at the University of British Columbia (UBC). The requested instrument, the Invitrogen Attune NxT coupled to an auto-sampler, will enable our team to perform and develop a broad series of assays for projects in natural science, in which large particles and different types of cells (bacteria, yeast, plant and mammalian) are quantified based on surface markers and/or fluorescent proteins. Currently, there is no high-throughput or sufficiently fast and sensitive equipment within the MSL that supports the proposed applications. Instead, the different team members have worked either with an outdated instrument that can no longer be updated or with instruments distantly located in the UBC flow facility, which precludes many of the proposed applications for practical reasons. Therefore, the requested instrument will be key, both to sustain the current programs and to facilitate the development of innovative approaches by a cluster of researchers who have clearly demonstrated excellence in their respective fields. Collectively, the applicants and main associated users have generated over 250 publications in the past 5 years. The requested instrument will be central for the training of highly qualified personnel (HQP) in biological sciences. MSL values equity, diversity and inclusion, and offers an environment with a great diversity of trainees both in term of gender, ethnical background and education. There are over 300 trainees in the MSL, including 48 in the applicants' and 82 in the main users' laboratories. We anticipate that the requested flow cytometer will participate to the training of a large pool of 75-125 researchers in the next five years that will be beneficial for the biotechnology sector. ***********