Ultrahigh performance liquid chromatography - triple quadruple mass spectrometer for quantitative chemical and biochemical analysis

我们申请资金购买一台超高效液相色谱三重四极杆质谱仪（UHPLC-TQMS）用于精确的化学定量。UHPLC-TQMS是一种完善的分析平台，具有极高的灵敏度、可重复性和精确的分子测量，具有很强的鲁棒性，是各种研究应用迫切需要的。该LC-MS在UBC化学质谱分析设备中运行和维护，将有助于解决UBC研究人员对定量化学，代谢物和蛋白质分析的高需求和未满足的需求。申请者来自化学系、动物系和土地与食品系统学院，并在代谢组学和质谱（陶焕）、天然产物生物合成（凯瑟琳·瑞安）、蛋白质生物化学（苏珊娜·Straus）、实时化学反应监测（杰森·海因）、化学生物标志物（大卫·陈）、微量有机污染物（艾伦·伯特伦）、比较生理学（凯蒂·马歇尔）和分子食品安全（芭芭拉·斯特凡斯卡）。除了为(1)靶向代谢组学、(2)痕量化学物质的检测和定量以及(3)蛋白质定量创造新的和维持现有的研究、合作和资助机会外，该仪器还将为至少91名高素质人员（HQP）提供实践培训。化学系认为全面质量管理体系是一项重要的采购，并为这项申请提供了大量的配套资金。该仪器将成为未来几十年的重要工作机器，通过HQP的培训，并通过促进化学等多个领域的卓越研究，为大学及其员工增加巨大价值。

We request funds to purchase an ultrahigh performance liquid chromatography triple quadrupole mass spectrometer (UHPLC-TQMS) for accurate chemical quantification. UHPLC-TQMS is a well-established analytical platform that provides extremely sensitive, reproducible, and precise molecular measurements with great robustness, which is urgently needed for a variety of research applications. Operated and maintained in the UBC Chemistry Mass Spectrometry Facility, this LC-MS will help address the high and unmet demand for quantitative chemical, metabolite, and protein analysis by researchers across UBC. Applicants are from the Departments of Chemistry, Zoology, and the Faculty of Land & Food Systems, and conduct important research in the areas of metabolomics and mass spectrometry (Tao Huan), natural product biosynthesis (Katherine Ryan), protein biochemistry (Suzana Straus), real-time chemical reaction monitoring (Jason Hein), chemical biomarkers (David Chen), trace levels of organic pollutants (Allan Bertram), comparative physiology (Katie Marshall) and molecular food safety (Barbara Stefanska). In addition to creating new and maintaining existing research, collaboration and funding opportunities for (1) targeted metabolomics, (2) detection and quantification of trace levels of chemicals, and (3) protein quantification, the instrument will also provide hands-on training for at least 91 highly qualified personnel (HQP). The Department of Chemistry has deemed a TQMS as a critical purchase and is supporting this application with substantial matching funds. The proposed instrument will be an important workhorse for the next several decades, adding great value to the university and its employees through the training of HQP and by fostering research excellence in several areas of chemistry and beyond.