Ultrahigh performance liquid chromatography - triple quadruple mass spectrometer for quantitative chemical and biochemical analysis

我们申请资金购买高效液相色谱三重四极杆质谱仪（UHPLC-TQMS），用于准确的化学定量。UHPLC-TQMS是一种成熟的分析平台，可提供极其灵敏、可重复和精确的分子测量，具有很强的鲁棒性，这是各种研究应用所迫切需要的。该LC-MS在UBC化学质谱设施中运行和维护，将有助于解决UBC研究人员对定量化学，代谢物和蛋白质分析的高需求和未满足的需求。申请人来自化学系，动物学系和土地与食品系统学院，并在代谢组学和质谱学领域进行重要研究（陶欢）、天然产物生物合成（凯瑟琳瑞恩），蛋白质生物化学（Suzana Straus），实时化学反应监测（杰森·海因）、化学生物标志物（大卫陈）、有机污染物的痕量水平（艾伦·伯特伦）、比较生理学（凯蒂·马歇尔）和分子食品安全（芭芭拉·斯特凡斯卡）。除了为（1）靶向代谢组学，（2）痕量化学品的检测和定量以及（3）蛋白质定量创造新的和维持现有的研究，合作和资助机会外，该仪器还将为至少91名高素质人员（HQP）提供实践培训。化学系已将TQMS视为一项关键采购，并以大量配套资金支持此申请。拟议的仪器将是未来几十年的重要主力，通过HQP的培训和促进化学及其他几个领域的卓越研究，为大学及其员工增加巨大价值。

We request funds to purchase an ultrahigh performance liquid chromatography triple quadrupole mass spectrometer (UHPLC-TQMS) for accurate chemical quantification. UHPLC-TQMS is a well-established analytical platform that provides extremely sensitive, reproducible, and precise molecular measurements with great robustness, which is urgently needed for a variety of research applications. Operated and maintained in the UBC Chemistry Mass Spectrometry Facility, this LC-MS will help address the high and unmet demand for quantitative chemical, metabolite, and protein analysis by researchers across UBC. Applicants are from the Departments of Chemistry, Zoology, and the Faculty of Land & Food Systems, and conduct important research in the areas of metabolomics and mass spectrometry (Tao Huan), natural product biosynthesis (Katherine Ryan), protein biochemistry (Suzana Straus), real-time chemical reaction monitoring (Jason Hein), chemical biomarkers (David Chen), trace levels of organic pollutants (Allan Bertram), comparative physiology (Katie Marshall) and molecular food safety (Barbara Stefanska). In addition to creating new and maintaining existing research, collaboration and funding opportunities for (1) targeted metabolomics, (2) detection and quantification of trace levels of chemicals, and (3) protein quantification, the instrument will also provide hands-on training for at least 91 highly qualified personnel (HQP). The Department of Chemistry has deemed a TQMS as a critical purchase and is supporting this application with substantial matching funds. The proposed instrument will be an important workhorse for the next several decades, adding great value to the university and its employees through the training of HQP and by fostering research excellence in several areas of chemistry and beyond.