Effects of cell culture plastics on dendritic cells activated using Immunyr(TM)

细胞培养塑料对使用 Immunyr(TM) 激活的树突状细胞的影响

• 批准号: 533984-2018
• 负责人: Hoesli, Corinne
• 金额: $ 10.7万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=698492
• 关键词: Effects; cell; culture; plastics; dendritic

Cancer immunotherapy has become an increasingly important therapeutic approach in oncology. It is defined as a means to activate a patient's own immune system to recognize and respond to a developing tumor. Our goal is to develop dendritic cell-based cancer vaccines. DCs are the "gatekeepers" of our immune system, and they can have the capacity to activate other immune cells to attack cancers. Kanyr Pharma has developed a technology called Immunyr(TM) that can improve the function of the dendritic cells in culture prior to reinjection into the patients. Kanyr Pharma currently produces these dendritic cells in plastic containers that are not completely closed. Liquids are introduced into the containers manually through caps and lids. For clinical production of the dendritic cells, it would be safer to culture the cells in closed plastic vessels such as bags filled through tubing. The use of closed cell culture vessels would help move dendritic cell based cancer vaccines into most university hospitals, reduce cost assure higher quality of the autologous dendritic cells produced for each patient. The main objective of this project is to study the effect of the transition to closed plastic vessels and different plastic materials on the function of dendritic cells obtained in the presence of Immunyr(TM) compounds.

癌症免疫疗法已成为肿瘤学中越来越重要的治疗方法。它被定义为激活患者自身免疫系统以识别和应对正在发展的肿瘤的一种手段。 我们的目标是开发基于树突状细胞的癌症疫苗。 DC 是我们免疫系统的“看门人”，它们有能力激活其他免疫细胞来攻击癌症。 Kanyr Pharma 开发了一种名为 Immunyr(TM) 的技术，可以在重新注射到患者体内之前改善培养物中的树突状细胞的功能。 Kanyr Pharma 目前在未完全封闭的塑料容器中生产这些树突状细胞。液体通过盖子和盖子手动引入容器中。对于树突状细胞的临床生产，在封闭的塑料容器（例如通过管道填充的袋子）中培养细胞会更安全。使用封闭细胞培养容器将有助于将基于树突状细胞的癌症疫苗转移到大多数大学医院，降低成本，确保为每位患者生产更高质量的自体树突状细胞。该项目的主要目标是研究向封闭塑料容器和不同塑料材料的转变对在存在 Immunyr(TM) 化合物的情况下获得的树突状细胞功能的影响。