Imaging system for live 3D tissue engineered constructs

我们正在要求一种光学深层组织成像系统，以成像人体规模的组织工程构造。拟议的成像模块由荧光*分子断层成像系统组成，能够对厘米级的活体组织进行3D近红外(NIR)荧光*成像。这项技术将使我们能够监测含有活细胞的厘米级组织中的细胞*健康和功能。我们和麦吉尔的其他人目前正在通过活细胞3D打印(生物打印)等技术*设计毫米到厘米厚的组织。在这种规模下，大多数组织必须进行血管处理，以避免缺氧和缺氧细胞死亡。拟议的组织成像*系统将允许我们通过光学探测器监测组织的健康和性能，例如检测代谢活动或局部氧分压或对其做出反应的染料。此外，表达近红外荧光报告蛋白的细胞系将被用于监测细胞数量、活力和功能。该系统将配备输液泵、气体流量控制器和温度控制系统，以实现对灌流的活组织的实时监测。通过控制气体混合物、培养基灌注率和温度，我们将能够评估这些参数对血氧分布和组织性能的影响。该系统与我们现有的微制造、组织工程和生物打印能力相结合，将创建一个完整的组织结构制造和监测平台。该系统将非常类似于用于小动物成像的仪器，但将专门为组织工程构建物成像而设计。该系统将*补充基于显微镜的系统，这些系统能够实现更高分辨率、更低*穿透性的光学成像，以及麦吉尔公司提供的医学成像方式(例如X射线、超声波、磁共振成像和核成像技术)*。来自我们实验室(超过50名受训人员)、合作者实验室、McGill的其他研究人员以及省(Thécell、魁北克先进材料中心、Proteo)和加拿大研究网络(加拿大生物材料学会、干细胞网络)的受训人员将可以访问这一共享设施。**

We are requesting an optical deep tissue*imaging system to image human-scale tissue engineered constructs. The proposed imaging module consists of a fluorescence*molecular tomography system enabling 3D near infrared (NIR) fluorescence*imaging of cm-scale live tissue. This technology will allow us to monitor cell*health and function in cm-scale tissues containing living cells. We and others*at McGill are currently engineering tissues of mm to cm thickness via techniques*such as live cell 3D printing (bioprinting). At this scale, most tissues must be vascularized to*avoid oxygen deprivation and hypoxic cell death. The proposed tissue imaging*system will allow us to monitor tissue health and performance through optical probes such as dyes that sense or respond to metabolic activity or local oxygen tension. Moreover, cell lines expressing NIR fluorescent reporter proteins will be used to monitor cell number, viability and function. The system will be equipped with an infusion*pump, gas flow controllers and a temperature control system to enable real-time*monitoring of perfused living tissues. By controlling the gas mixture, the culture medium perfusion rate*and the temperature, we will be able to assess the effects of these parameters on oxgen profiles and tissue performance. The combination of this system with our existing microfabrication, tissue engineering and bioprinting capabilities will create a complete tissue construct fabrication and monitoring platform. The system will be very similar to*instruments used for small animal imaging, but will be designed specifically to image tissue engineered constructs. The system will*complement microscopy-based systems that enable higher-resolution lower*penetration optical imaging, as well as medical imaging modalities (e.g. X-ray,*ultrasound, magnetic resonance imaging and nuclear imaging techniques)*available at McGill. Trainees from our laboratories (over 50 trainees), from the laboratories of collaborators, from other researchers at McGill as well as provincial (ThéCell, Québec Center for Advanced Materials, PROTEO) and Canadian research networks (Canadian Biomaterials Society, Stem Cell Network) will have access to this shared facility.**