Lab-on-smartphone with optical interface for rapid multiplex in-field testing of COVID-19

具有光学接口的智能手机实验室，用于快速多重现场测试 COVID-19

• 批准号: 555157-2020
• 负责人: Mahshid, Sara
• 金额: $ 3.64万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=705776
• 关键词: Lab; smartphone; optical; interface; rapid

Since the beginning of COVID-19 pandemic, over 5,800,000 confirmed cases and 360,000 deaths have been identified. The shortage of approved diagnostics tests in hospitals particularly in rural areas, have limited the access of individuals to diagnostic tests. We gather a team of experts at McGill University and Nikon Canada Inc. to develop a portable and cost-effective diagnostics platform for detection of SARS-CoV-2 (the virus causing COVID-19) with capability of rapid implementation in remote-locations and rural areas. Our team has already begun development of a prototype for testing of SARS-CoV-2 RNA via a novel colorimetric assay in a quantifiable multiplex fashion. Here, we implement our novel colorimetric fluidic device for portable multiplex testing of SARS-CoV-2 in minutes and interpretation of the test results with the help of a smartphone (data acquisition system). Our aim is to provide easy access to the results of diagnostics in remote locations and avoid overloading hospitals and lab facilities. With the support from Nikon (in-kind contribution), we will develop a portable and miniaturized imaging box composed of our novel colorimetric fluidic device and optical components capable of holding to smartphones, independent from their model (Aim1). We will then implement this portable and multiplex approach for testing SARS-CoV-2 from swab samples in a non-clinical simulated environment (Aim2). Mahshid Lab in the Department of Bioengineering, has already received all necessary protocols and McGill EHS approval "Use Biohazardous Materials" to work with SARS-CoV-2. Finally, we will develop a smartphone application (Android studio) for easing data interpretation and analysing the colorimetric data (color images) into reading signals (Aim3). Based on the availability of smartphones, being able to use these smart devices will put less pressure on the healthcare system and reduce the need for bulky computers especially for in-field testing or in rural areas. The potential to increase COVID-19 testing rate at a low cost will benefit the Canadian health system during the current pandemic and help prevent similar pandemics in the future.

自2019冠状病毒病大流行开始以来，已确认超过5，800，000例确诊病例及360，000例死亡病例。医院，特别是农村地区的医院缺乏经批准的诊断测试，限制了个人获得诊断测试的机会。我们聚集了麦吉尔大学和尼康加拿大公司的专家团队。开发一种便携式和具有成本效益的诊断平台，用于检测SARS-CoV-2（引起COVID-19的病毒），并能够在偏远地区和农村地区快速实施。我们的团队已经开始开发一种原型，用于通过一种新型的可量化的多重比色法来检测SARS-CoV-2 RNA。在这里，我们实现了我们的新型比色流体设备，用于在几分钟内对SARS-CoV-2进行便携式多重检测，并在智能手机（数据采集系统）的帮助下解释检测结果。我们的目标是在偏远地区提供方便的诊断结果，避免医院和实验室设施超负荷。在尼康的支持下（实物捐助），我们将开发一种便携式小型成像盒，该成像盒由我们的新型比色流体设备和光学组件组成，能够独立于智能手机型号（Aim 1）。然后，我们将实施这种便携式和多重方法，用于在非临床模拟环境中检测拭子样本中的SARS-CoV-2（Aim 2）。生物工程系的Mahshid实验室已经收到了所有必要的协议和麦吉尔EHS批准“使用生物危害材料”，以处理SARS-CoV-2。最后，我们将开发一个智能手机应用程序（Android工作室），用于简化数据解释和分析比色数据（彩色图像）到阅读信号（Aim 3）。基于智能手机的可用性，能够使用这些智能设备将减少对医疗保健系统的压力，并减少对大型计算机的需求，特别是在现场测试或农村地区。以低成本提高COVID-19检测率的潜力将有利于加拿大卫生系统在当前的大流行期间，并有助于预防未来类似的大流行。