High Sensitivity Electrochemical Biosensors for Biological Contamination in Water

用于水中生物污染的高灵敏度电化学生物传感器

• 批准号: 530394-2018
• 负责人: Mahshid, Sara
• 金额: $ 1.82万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=658474
• 关键词: Sensitivity; Electrochemical; Biosensors; Biological; Contamination

Monitoring the quality of water has global impact on the human health and environment. There are parameters**encountered for water quality including pH, heavy metals contamination, total dissolved solids (TDS), and**biological contamination such as bacteria. EnviroSen Inc. has successfully developed sensor prototypes based**on nanomaterials printed electrodes for monitoring residential water filtration performance by online measuring**of pH, heavy metals and TDS. The company is now developing a prototype for detection of E. coli bacteria in**municipal water distribution system, which requires different modifications on the existing prototype including**biological recognition elements to improve the sensitivity and the limit of detection at the point of use. This**NSERC Engage project will be the first collaborative effort between EnviroSen Inc and Prof. Sara Mahshid of**McGill University to develop an optimized electrochemical biosensor based on integration of graphene oxide**(GO)- an essential component of EnviroSen Inc's prototypes- and DNA-affinity assays for detection of E. Coli**in water. This Engage project will develop approaches for improving the surface functionality of GO printed**electrodes for immobilization of DNA signaling probes as the first essential step in improving the sensitivity of**detection of E. Coli. The Engage project will investigate (1) a new approach based on graphene drop-cast on**EnviroSen Inc's printed electrodes to enhance detection sites for DNA immobilization; (2) surface**modification/ functionalization of GO electrodes with metal nanoparticles for immobilization of DNA probes;**(3) direct capture and detection of E. Coli in real industrial condition. At the end of month 6 we will have**successfully integrated the optimized DNA-GO nanostructured electrode with EnviroSen Inc's sensor prototype**and implemented for on-line monitoring of E. Coli in city water. The project leverages based on skills**developed in Mahshid lab and at EnviroSen Inc and provide EnviroSen with valuable knowledge and feedback**to advance their prototypes for high-end applications in environmental sensing. EnviroSen Inc. provides $5K of**in-kind contributions in the form of staff salary, training and supplies to support the porject.

水质监测对人类健康和环境具有全球性影响。水质参数 ** 包括pH值、重金属污染、总溶解固体（TDS）和 ** 生物污染（如细菌）。EnviroSen Inc.已成功开发出基于纳米材料印刷电极的传感器原型，用于通过在线测量 ** pH值、重金属和TDS来监测住宅水过滤性能。该公司目前正在开发一种检测大肠杆菌的原型。城市供水系统中的大肠杆菌，这需要对现有原型进行不同的修改，包括 ** 生物识别元件，以提高使用点的灵敏度和检测限。这个 **NSERC Engage项目将是EnviroSen公司和 ** 麦吉尔大学的Sara Mahshid教授之间的第一个合作项目，旨在开发一种优化的电化学生物传感器，该生物传感器基于氧化石墨烯 **（GO）-EnviroSen公司原型的重要组成部分-和DNA亲和分析，用于检测E。水中的大肠杆菌。该Engage项目将开发用于改善GO印刷 ** 电极的表面功能的方法，用于固定DNA信号探针，作为提高E ** 检测灵敏度的第一个重要步骤。杆菌Engage项目将研究（1）一种基于石墨烯滴铸在 **EnviroSen Inc的印刷电极上的新方法，以增强DNA固定的检测位点;（2）用金属纳米颗粒对GO电极进行表面 ** 修饰/功能化，以固定DNA探针;**（3）直接捕获和检测E.大肠杆菌在真实的工业条件下。在6月底，我们将 ** 成功地将优化的DNA-GO纳米结构电极与EnviroSen公司的传感器原型 ** 集成，并用于在线监测大肠杆菌。城市水里有大肠杆菌。该项目利用Mahshid实验室和EnviroSen Inc开发的技能 **，为EnviroSen提供宝贵的知识和反馈 **，以推进其环境传感高端应用的原型。EnviroSen Inc.以员工工资、培训和供应品的形式提供了5000美元的实物捐助，以支持该项目。