Regulation of 5-methylcytosine upon the transition from naive to primed pluripotency

从初始多能性向引发多能性转变时 5-甲基胞嘧啶的调节

• 批准号: RGPIN-2018-04856
• 负责人: Pastor, William
• 金额: $ 3.42万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=713224
• 关键词: Regulation; methylcytosine; upon; transition; naive

During human development, it is critical that certain genes be activated and inactivated at the right time. DNA methylation helps control this process. Certain enzymes called DNA methyltransferases chemically modify DNA to silence genes that need to be turned off for development to occur correctly. I will use stem cells to study how DNA methyltransferases are regulated during stages of early human development that would otherwise be impossible to study. My research will be directly relevant to certain diseases, such as cancer and ICF syndrome, where aberrations of DNA methylation occur and will more broadly help us understand how early human development is controlled. Additionally, I will train a Ph.D. student to become an expert in both bench science and computational analysis.

在人类发育过程中，某些基因在正确的时间被激活和失活是至关重要的。DNA甲基化有助于控制这一过程。某些被称为DNA甲基转移酶的酶可以化学修饰DNA，使需要关闭才能正常发育的基因沉默。我将使用干细胞来研究DNA甲基转移酶在人类早期发育阶段是如何调控的，否则这是不可能研究的。我的研究将与某些疾病直接相关，如癌症和ICF综合征，这些疾病会发生DNA甲基化异常，并将更广泛地帮助我们了解人类早期发育是如何控制的。此外，我还将培养一名博士生，使其成为实验室科学和计算分析方面的专家。