Biomechanics and Interrelations of Microtubules and Intracellular Trafficking: A Guide for Characterizing Carbon Nanotubes Cytotoxicity

微管和细胞内运输的生物力学和相互关系：表征碳纳米管细胞毒性的指南

• 批准号: RGPIN-2018-05892
• 负责人: Alisaraie, Laleh
• 金额: $ 2.33万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=713939
• 关键词: Biomechanics; Interrelations; Microtubules; Intracellular; Trafficking

Carbon nanotubes (CNTs) are tubular cylinders of carbon atoms that have remarkable mechanical, electrical, thermal, optical and chemical properties. Their versatility allows using them in a wide range of industries including field emission, energy storage, super-strong fabrics, coating and filming, and cost-effective air and water filtration. With the increase in application of these nanomaterials, biological and environmental exposures to CNTs are also increased. However, we know very little about how CNTs can affect cellular processes and pose biological risks to living organisms. Microtubules are involved in many processes in a cell, including cell growth, division, and programmed cell death. They are one of the main components of the cell “skeleton”. CNTs have major structural similarities to microtubules. Due to these similarities, microtubules and their associated proteins may be prone to interacting with CNTs that are taken up by cells, and this has the potential to disrupt their natural function. Our research will look at microtubules and some of their associated proteins whose function depends on binding to microtubules, to learn more about yet unknown features of these subcellular systems. We will also explore the specific mechanism of how CNTs can disturb these biological systems and find the relationship to the chemical structure of CNTs. With this information, we will be able to modify CNTs, either structurally or chemically, to reduce the risk of them interfering with essential cellular processes. We can apply this knowledge in the design of CNT-based nanomaterials that are safer for living organisms and ecosystems. This research program will create a great platform for bringing together national and international researchers from biochemistry, cell biology, biomaterial engineering, toxicology and computing science providing a rich training environment and opportunities for a new generation of young scientists who will work as undergraduate or graduate students in this interdisciplinary research program. They will develop advanced and specialized skills that will prepare them well to work in this exciting emerging field in industry or academia.

碳纳米管是由碳原子组成的管状圆柱体，具有显著的力学、电学、热学、光学和化学性质。它们的多功能性使其可以广泛用于各种行业，包括场发射、储能、超强织物、涂层和成膜，以及经济实惠的空气和水过滤。随着这些纳米材料应用的增加，生物和环境对碳纳米管的暴露也增加了。然而，我们对碳纳米管如何影响细胞过程并对生物构成生物风险知之甚少。 微管参与细胞的许多过程，包括细胞生长、分裂和程序性细胞死亡。它们是细胞“骨架”的主要组成部分之一。碳纳米管在结构上与微管有很大相似之处。由于这些相似性，微管及其相关蛋白可能容易与被细胞摄取的碳纳米管相互作用，这有可能破坏它们的自然功能。我们的研究将着眼于微管和它们的一些相关蛋白质，它们的功能依赖于与微管的结合，以了解更多关于这些亚细胞系统未知的特征。我们还将探索碳纳米管干扰这些生物系统的具体机制，并找出与碳纳米管化学结构的关系。有了这些信息，我们将能够对碳纳米管进行结构或化学修饰，以降低它们干扰基本细胞过程的风险。我们可以将这些知识应用于基于碳纳米管的纳米材料的设计中，这种材料对活体和生态系统更安全。 这一研究计划将为汇聚来自生物化学、细胞生物学、生物材料工程、毒理学和计算机科学的国内外研究人员创造一个很好的平台，为将在这一跨学科研究计划中作为本科生或研究生工作的新一代年轻科学家提供丰富的培训环境和机会。他们将发展先进和专门的技能，为他们在这一令人兴奋的新兴工业或学术界领域工作做好准备。