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Investigating the effects of electronic cigarettes and cannabis smoke on buccal microbes

研究电子烟和大麻烟雾对口腔微生物的影响

基本信息

批准号：
RGPIN-2019-04475
负责人：
Rouabhia, Mahmoud
金额：
$ 2.33万
依托单位：
Université Laval
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2020
资助国家：
加拿大
起止时间：
2020-01-01 至 2021-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=714901
关键词：
Investigating effects electronic cigarettes cannabis

项目摘要

In nature, microbes organize themselves to overcome any inappropriate situation. They begin by adhering to various surfaces to form a biofilm consisting of a gathering of bacteria embedded in self-derived compounds. Environmental conditions have significant effects on microbial biofilm formation. It is well known that combustible cigarette smoke (CCS) is one factor with a significant impact on oral cavity microbial ecosystems. Several studies indicated that smoking increases the adhesion of S mutans to different surfaces including restorative dental acrylic resin and tooth brackets. It has also been shown that CCS promoted the growth and biofilm formation of P. gingivalis. As part of the oral microbial ecosystem, C. albicans is also subjected to the effect of cigarette smoke. We demonstrated that CCS activated different virulent genes expressed by C. albicans. CCS may not be the only stressful factor leading to buccal microbes' growth and biofilm formation. The e-cigarette, as well as cannabis smoke, may also promote such buccal microbial growth and biofilm formation. This research program is to investigate the interactions of e-cigarette vapors and cannabis smoke with two buccal pathogenic microbes, namely C. albicans and S. mutans. The research program consists of following 4 specific objectives: (i) To investigate the effect of e-cigarette vapors and cannabis smoke on C. albicans growth, biofilm formation and whole genome transcription. (ii) To investigate the effect of e-cigarette vapors and cannabis smoke on S. mutans growth, biofilm formation and whole genome transcription. (iii) To investigate the virulence/pathogenicity of the e-cigarette vapor or cannabis smoke exposed C. abicans. (iv) To investigate the virulence/pathogenicity of the e-cigarette vapor or cannabis smoke exposed S. mutans. To my best knowledge, very few studies, if any, investigated the effect of e-cigarette vapors or cannabis smoke on buccal microbes. This makes our research program unique and highly innovative. Significance: With this research program we will be able to elucidate buccal microorganisms' behaviors under the influence of e-cigarette vapor or cannabis smoke. This research program will contribute to future preventative

在自然界中，微生物会自我组织以克服任何不适当的情况。它们首先粘附在各种表面上，形成生物膜，该生物膜由嵌入自源化合物中的细菌聚集体组成。环境条件对微生物生物膜的形成有显着影响。众所周知，可燃香烟烟雾（CCS）是对口腔微生物生态系统产生重大影响的因素之一。多项研究表明，吸烟会增加变形链球菌对不同表面的粘附，包括修复性牙科丙烯酸树脂和牙托。研究还表明，CCS 促进牙龈卟啉单胞菌的生长和生物膜形成。作为口腔微生物生态系统的一部分，白色念珠菌也受到香烟烟雾的影响。我们证明 CCS 激活了白色念珠菌表达的不同毒力基因。 CCS 可能不是导致口腔微生物生长和生物膜形成的唯一压力因素。电子烟以及大麻烟雾也可能促进口腔微生物的生长和生物膜的形成。该研究计划旨在研究电子烟蒸汽和大麻烟雾与两种口腔致病微生物（即白色念珠菌和变形链球菌）的相互作用。该研究计划包括以下 4 个具体目标： (i) 研究电子烟蒸汽和大麻烟雾对白色念珠菌生长、生物膜形成和全基因组转录的影响。 (ii) 研究电子烟蒸汽和大麻烟雾对变形链球菌生长、生物膜形成和全基因组转录的影响。 (iii) 研究接触电子烟蒸气或大麻烟雾的 C. abicans 的毒力/致病性。 (iv) 研究暴露于电子烟蒸气或大麻烟雾的变形链球菌的毒力/致病性。据我所知，很少有研究（如果有的话）调查电子烟蒸气或大麻烟雾对口腔微生物的影响。这使得我们的研究项目独一无二且极具创新性。意义：通过这项研究计划，我们将能够阐明口腔微生物在电子烟蒸汽或大麻烟雾影响下的行为。该研究计划将有助于未来的预防