Bioprocess Development for the Large Scale Production of Extracellular Vesicles From Adult Stem Cells

从成体干细胞大规模生产细胞外囊泡的生物工艺开发

基本信息

  • 批准号:
    RGPIN-2019-07196
  • 负责人:
  • 金额:
    $ 2.84万
  • 依托单位:
  • 依托单位国家:
    加拿大
  • 项目类别:
    Discovery Grants Program - Individual
  • 财政年份:
    2020
  • 资助国家:
    加拿大
  • 起止时间:
    2020-01-01 至 2021-12-31
  • 项目状态:
    已结题

项目摘要

Stem cells exist in very small numbers in almost all tissues within the body. For example, mesenchymal stem cells (MSCs) are a type of adult stem cell that can be found in bone marrow and fat. There is huge interest in MSCs because their implantation into a damaged tissue often leads to some degree of functional recovery. Despite there being significant barriers (e.g. regulatory issues, cost) to the widespread clinical use of MSCs, hundreds of clinical trials have been undertaken to investigate how MSCs can be used to treat medical conditions such as heart disease, stroke and type I diabetes. Evidence now suggests that many of the positive outcomes observed after MSC implantation are due to the products they secrete. Of particular interest are extracellular vesicles (EVs), small spherical structures secreted by MSCs that contain cargo composed of bioactive molecules. EVs have been observed to migrate from the cell where they were produced and deliver their cargo to target cells, causing the target cells to perform a desired action, such as initiating a repair process. Thus, MSC derived EVs (MSC-EVs) have the potential to be administered clinically as effective therapeutic agents, an approach with fewer barriers compared to MSC implantation. Moreover, EVs are now known to be naturally produced in all of us, from the time we are embryos to the time that we die. They are believed to serve as a complex communication system between cells and are implicated in the processes of tissue development, function and aging. Thus, researchers can use EVs to learn more about these processes in healthy individuals and understand how changes in these processes can lead to birth defects, tissue degeneration and disease. The field of EVs is currently in its infancy and primed for explosive growth. A huge challenge hampering progress is a lack of defined EVs with which researchers can work. Because they can't be easily obtained directly from tissues, researchers collect EVs made by MSCs that are grown outside the body in vessels which contain a liquid medium made up of ingredients that encourage isolated MSCs to divide and secrete EVs. Standard large-scale EV production methods do not yet exist, and current methods to collect EVs from the medium are laborious, time consuming and have low recoveries. We have an established biomanufacturing research program in which we have previously developed robust technologies to scale-up stem cell production in large, controlled vessels called bioreactors. Here we propose to extend our program, and over the next 5 years, (i) engineer standard methods for the large-scale production of defined, functional MSC-EV populations in bioreactors, and (ii) design a novel device capable of rapidly collecting EVs from medium. Outcomes of this work will be the creation of novel technologies that support EV production, study and application development, and the training of individuals qualified to work in this rapidly emerging new field.
干细胞的数量非常少,存在于人体几乎所有组织中。例如,间充质干细胞(MSCs)是一种可以在骨髓和脂肪中发现的成体干细胞。人们对间充质干细胞非常感兴趣,因为将其植入受损组织中通常会导致一定程度的功能恢复。尽管在临床上广泛使用间充质干细胞存在重大障碍(例如监管问题、成本),但已经开展了数百项临床试验,以研究如何使用间充质干细胞治疗心脏病、中风和I型糖尿病等疾病。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Sen, Arindom其他文献

Using bacterial bioluminescence to evaluate the impact of biofilm on porous media hydraulic properties
  • DOI:
    10.1016/j.mimet.2014.11.015
  • 发表时间:
    2015-02-01
  • 期刊:
  • 影响因子:
    2.2
  • 作者:
    Bozorg, Ali;Gates, Ian D.;Sen, Arindom
  • 通讯作者:
    Sen, Arindom
Biodegradation of Polymers Used in Oil and Gas Operations: Towards Enzyme Biotechnology Development and Field Application.
  • DOI:
    10.3390/polym14091871
  • 发表时间:
    2022-05-03
  • 期刊:
  • 影响因子:
    5
  • 作者:
    Berdugo-Clavijo, Carolina;Scheffer, Gabrielle;Sen, Arindom;Gieg, Lisa M.
  • 通讯作者:
    Gieg, Lisa M.
High temperature utilization of PAM and HPAM by microbial communities enriched from oilfield produced water and activated sludge
  • DOI:
    10.1186/s13568-019-0766-9
  • 发表时间:
    2019-04-09
  • 期刊:
  • 影响因子:
    3.7
  • 作者:
    Berdugo-Clavijo, Carolina;Sen, Arindom;Gieg, Lisa M.
  • 通讯作者:
    Gieg, Lisa M.
Real time monitoring of biofilm development under flow conditions in porous media
  • DOI:
    10.1080/08927014.2012.723204
  • 发表时间:
    2012-01-01
  • 期刊:
  • 影响因子:
    2.7
  • 作者:
    Bozorg, Ali;Gates, Ian D.;Sen, Arindom
  • 通讯作者:
    Sen, Arindom
Identification of growth and attachment factors for the serum-free isolation and expansion of human mesenchymal stromal cells
  • DOI:
    10.3109/14653249.2010.495113
  • 发表时间:
    2010-01-01
  • 期刊:
  • 影响因子:
    4.5
  • 作者:
    Jung, Sunghoon;Sen, Arindom;Behie, Leo A.
  • 通讯作者:
    Behie, Leo A.

Sen, Arindom的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Sen, Arindom', 18)}}的其他基金

Bioprocess Development for the Large Scale Production of Extracellular Vesicles From Adult Stem Cells
从成体干细胞大规模生产细胞外囊泡的生物工艺开发
  • 批准号:
    RGPIN-2019-07196
  • 财政年份:
    2022
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production of Extracellular Vesicles From Adult Stem Cells
从成体干细胞大规模生产细胞外囊泡的生物工艺开发
  • 批准号:
    RGPIN-2019-07196
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bionanoparticle Analysis System
生物纳米颗粒分析系统
  • 批准号:
    RTI-2021-00252
  • 财政年份:
    2020
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Research Tools and Instruments
Bioprocess Development for the Large Scale Production of Extracellular Vesicles From Adult Stem Cells
从成体干细胞大规模生产细胞外囊泡的生物工艺开发
  • 批准号:
    RGPIN-2019-07196
  • 财政年份:
    2019
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2018
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2016
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Assessing the feasibility of enzyme technology for the biodegradation of polymers used in hydraulic fracturing operations
评估酶技术用于水力压裂作业中聚合物生物降解的可行性
  • 批准号:
    469717-2014
  • 财政年份:
    2016
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Collaborative Research and Development Grants
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2015
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Assessing the feasibility of enzyme technology for the biodegradation of polymers used in hydraulic fracturing operations
评估酶技术用于水力压裂作业中聚合物生物降解的可行性
  • 批准号:
    469717-2014
  • 财政年份:
    2015
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Collaborative Research and Development Grants
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2014
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual

相似国自然基金

水稻边界发育缺陷突变体abnormal boundary development(abd)的基因克隆与功能分析
  • 批准号:
    32070202
  • 批准年份:
    2020
  • 资助金额:
    58 万元
  • 项目类别:
    面上项目
Development of a Linear Stochastic Model for Wind Field Reconstruction from Limited Measurement Data
  • 批准号:
  • 批准年份:
    2020
  • 资助金额:
    40 万元
  • 项目类别:

相似海外基金

Bioprocess Development for the Large Scale Production of Extracellular Vesicles From Adult Stem Cells
从成体干细胞大规模生产细胞外囊泡的生物工艺开发
  • 批准号:
    RGPIN-2019-07196
  • 财政年份:
    2022
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production of Extracellular Vesicles From Adult Stem Cells
从成体干细胞大规模生产细胞外囊泡的生物工艺开发
  • 批准号:
    RGPIN-2019-07196
  • 财政年份:
    2021
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production of Extracellular Vesicles From Adult Stem Cells
从成体干细胞大规模生产细胞外囊泡的生物工艺开发
  • 批准号:
    RGPIN-2019-07196
  • 财政年份:
    2019
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2018
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2016
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2015
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2014
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess Development for the Large Scale Production and Differentiation of Adult Human Stem Cells
成人干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2013
  • 财政年份:
    2013
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess development for the large scale production and differentiation of mammalian adult stem cells
哺乳动物成体干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2008
  • 财政年份:
    2012
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
Bioprocess development for the large scale production and differentiation of mammalian adult stem cells
哺乳动物成体干细胞大规模生产和分化的生物工艺开发
  • 批准号:
    288129-2008
  • 财政年份:
    2011
  • 资助金额:
    $ 2.84万
  • 项目类别:
    Discovery Grants Program - Individual
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了